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Cited by 445DNA Repair MechanismsBacterial Genetics and BiotechnologyGenomics and Phylogenetic Studies

The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.

Reassembly of shattered chromosomes in Deinococcus radiodurans

Zahradka, Ksenija, Slade, Dea, Bailone, Adriana, Sommer, Suzanne, Averbeck, Dietrich, Petranovic, Mirjana, Lindner, Ariel B., Radman, Miroslav·2006·Nature
DOI 10.1038/nature05160
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Abstract

Dehydration or desiccation is one of the most frequent and severe challenges to living cells. The bacterium Deinococcus radiodurans is the best known extremophile among the few organisms that can survive extremely high exposures to desiccation and ionizing radiation, which shatter its genome into hundreds of short DNA fragments. Remarkably, these fragments are readily reassembled into a functional 3.28-megabase genome. Here we describe the relevant two-stage DNA repair process, which involves a previously unknown molecular mechanism for fragment reassembly called 'extended synthesis-dependent strand annealing' (ESDSA), followed and completed by crossovers. At least two genome copies and random DNA breakage are requirements for effective ESDSA. In ESDSA, chromosomal fragments with overlapping homologies are used both as primers and as templates for massive synthesis of complementary single strands, as occurs in a single-round multiplex polymerase chain reaction. This synthesis depends on DNA polymerase I and incorporates more nucleotides than does normal replication in intact cells. Newly synthesized complementary single-stranded extensions become 'sticky ends' that anneal with high precision, joining together contiguous DNA fragments into long, linear, double-stranded intermediates. These intermediates require RecA-dependent crossovers to mature into circular chromosomes that comprise double-stranded patchworks of numerous DNA blocks synthesized before radiation, connected by DNA blocks synthesized after radiation.

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Metadata

Authors
Zahradka, K., Slade, D., Bailone, A., Sommer, S., Averbeck, D., Petranovic, M., Lindner, A. B., & Radman, M.
Affiliations
  • Inserm (FR)
  • Université Paris-Sud (FR)
  • Centre National de la Recherche Scientifique (FR)
Corresponding author
Radman, Miroslav
ORCID
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2006-09-27
Volume
443
Issue
7111
Pages
569-573
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/nature05160
OpenAlex
W2036099589
Cited by
444
References
30
Citations by year
2026: 13 · 2025: 16 · 2024: 14 · 2023: 27 · 2022: 12 · 2021: 24 · 2020: 24 · 2019: 21 · 2018: 19 · 2017: 13 · 2016: 18 · 2015: 19 · 2014: 26 · 2013: 22 · 2012: 22
FWCI
12.85
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords
Deinococcus radiodurans, radioresistance (biology), DNA repair, homologous recombination, DNA polymerase I, single-stranded DNA synthesis, genome fragmentation, desiccation tolerance, extremophiles
MeSH terms
Bromodeoxyuridine, Chromosomes, Bacterial, Desiccation, DNA Damage, DNA Polymerase I, DNA Repair, DNA Replication, DNA, Bacterial, Models, Genetic, Photolysis, Radiation Tolerance, Genome, Bacterial, Deinococcus

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