The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Gene duplication and the adaptive evolution of a classic genetic switch
Abstract
How gene duplication and divergence contribute to genetic novelty and adaptation has been of intense interest, but experimental evidence has been limited. The genetic switch controlling the yeast galactose use pathway includes two paralogous genes in Saccharomyces cerevisiae that encode a co-inducer (GAL3) and a galactokinase (GAL1). These paralogues arose from a single bifunctional ancestral gene as is still present in Kluyveromyces lactis. To determine which evolutionary processes shaped the evolution of the two paralogues, here we assess the effects of precise replacement of coding and non-coding sequences on organismal fitness. We suggest that duplication of the ancestral bifunctional gene allowed for the resolution of an adaptive conflict between the transcriptional regulation of the two gene functions. After duplication, previously disfavoured binding site configurations evolved that divided the regulation of the ancestral gene into two specialized genes, one of which ultimately became one of the most tightly regulated genes in the genome.
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“Microbial metabolic networks often provide appropriate functions at a minimal size [134] and furthermore, their complexity seems to be correlated with environmental factors [135].”
From Adaptive laboratory evolution—Principles and applications for biotechnology (2013) · cites this paper as [134]
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Conant, G. C., & Wolfe, K. H. (2008) · Nature Reviews Genetics · doi:10.1038/nrg2482 · Cited by 1,281
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Pigliucci, M., & Müller, G. (2010) · The MIT Press eBooks · doi:10.7551/mitpress/9780262513678.001.0001 · Cited by 976
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Dragosits, M., & Mattanovich, D. (2013) · Microbial Cell Factories · doi:10.1186/1475-2859-12-64 · Cited by 827
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Metadata
- Authors
- Hittinger, C. T., & Carroll, S. B.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Carroll, Sean B.
- Venue
- Nature
- Published
- 2007-10
- Volume
- 449
- Issue
- 7163
- Pages
- 677-681
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1996490834
- Cited by
- 439
- References
- 51
- Citations by year
- 2026: 7 · 2025: 14 · 2024: 26 · 2023: 24 · 2022: 21 · 2021: 23 · 2020: 17 · 2019: 22 · 2018: 17 · 2017: 28 · 2016: 20 · 2015: 30 · 2014: 27 · 2013: 25 · 2012: 27
- FWCI
- 11.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Keywords
- gene duplication, gene divergence, adaptive evolution, genetic novelty, genetic switch, galectin-3, GAL1, paralogs, transcriptional regulation, Saccharomyces cerevisiae, Kluyveromyces lactis, fitness
- MeSH terms
- Base Sequence, Binding Sites, DNA-Binding Proteins, Galactokinase, Models, Genetic, Molecular Sequence Data, Promoter Regions, Genetic, Saccharomyces cerevisiae, Transcription Factors, Gene Expression Regulation, Fungal, Evolution, Molecular, Genes, Duplicate, Gene Duplication, Saccharomyces cerevisiae Proteins
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