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Regulation of cell cycle progression and gene expression by H2A deubiquitination

Joo, Heui-Yun, Zhai, Ling, Yang, Chunying, Nie, Shuyi, Erdjument-Bromage, Hediye, Tempst, Paul, Chang, Chenbei, Wang, Hengbin·2007·Nature
DOI 10.1038/nature06256
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Abstract

Post-translational histone modifications have important regulatory roles in chromatin structure and function. One example of such modifications is histone ubiquitination, which occurs predominately on histone H2A and H2B. Although the recent identification of the ubiquitin ligase for histone H2A has revealed important roles for H2A ubiquitination in Hox gene silencing as well as in X-chromosome inactivation, the enzyme(s) involved in H2A deubiquitination and the function of H2A deubiquitination are not known. Here we report the identification and functional characterization of the major deubiquitinase for histone H2A, Ubp-M (also called USP16). Ubp-M prefers nucleosomal substrates in vitro, and specifically deubiquitinates histone H2A but not H2B in vitro and in vivo. Notably, knockdown of Ubp-M in HeLa cells results in slow cell growth rates owing to defects in the mitotic phase of the cell cycle. Further studies reveal that H2A deubiquitination by Ubp-M is a prerequisite for subsequent phosphorylation of Ser 10 of H3 and chromosome segregation when cells enter mitosis. Furthermore, we demonstrate that Ubp-M regulates Hox gene expression through H2A deubiquitination and that blocking the function of Ubp-M results in defective posterior development in Xenopus laevis. This study identifies the major deubiquitinase for histone H2A and demonstrates that H2A deubiquitination is critically involved in cell cycle progression and gene expression.

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Crossref · 10.1038/nature06256

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  • USP16 is an antagonist of BMI1 and a de-repressor of Cdkn2a that acts through enzymatic removal of ubiquitin from histone H2A.

    “Upstream of Cdkn2a is USP16, an antagonist of BMI1 and a de-repressor of Cdkn2a that acts through the enzymatic removal of ubiquitin from histone H2A (Figure 4C; Adorno et al., 2013; Joo et al., 2007).”

    From Inhibiting USP16 rescues stem cell aging and memory in an Alzheimer’s model (2022) · cites this paper as Joo et al., 2007

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Research works printed but not linked: 29

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Metadata

Authors
Joo, H.-Y., Zhai, L., Yang, C., Nie, S., Erdjument-Bromage, H., Tempst, P., Chang, C., & Wang, H.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2007-10
Volume
449
Issue
7165
Pages
1068-1072
ISSN
  • 0028-0836
  • 1476-4687
DOI
10.1038/nature06256
Cited by
286
References
0
Language
en
Licence
www.springer.com/tdm (tdm)

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