The DOI matches its Crossref record; 46 of the 46 references it cites were found in a registry or an index.
RNA polymerase II–TFIIB structure and mechanism of transcription initiation
Open accessAbstract
To initiate gene transcription, RNA polymerase II (Pol II) requires the transcription factor IIB (B). Here we present the crystal structure of the complete Pol II–B complex at 4.3 Å resolution, and complementary functional data. The results indicate the mechanism of transcription initiation, including the transition to RNA elongation. Promoter DNA is positioned over the Pol II active centre cleft with the ‘B-core’ domain that binds the wall at the end of the cleft. DNA is then opened with the help of the ‘B-linker’ that binds the Pol II rudder and clamp coiled-coil at the edge of the cleft. The DNA template strand slips into the cleft and is scanned for the transcription start site with the help of the ‘B-reader’ that approaches the active site. Synthesis of the RNA chain and rewinding of upstream DNA displace the B-reader and B-linker, respectively, to trigger B release and elongation complex formation.
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Cites
Structural Basis of Transcription: RNA Polymerase II at 2.8 Ångstrom Resolution
Cramer, P., Bushnell, D. A., & Kornberg, R. D. (2001) · Science · doi:10.1126/science.1059493 · Cited by 1,247
Self-citationThe role of general initiation factors in transcription by RNA polymerase II
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Structural Basis of Transcription: An RNA Polymerase II Elongation Complex at 3.3 Å Resolution
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Self-citationCrystal structure of a bacterial RNA polymerase holoenzyme at 2.6 Å resolution
Vassylyev, D. G., Sekine, S.-I., Laptenko, O., Lee, J., Vassylyeva, M. N., Borukhov, S., & Yokoyama, S. (2002) · Nature · doi:10.1038/nature752 · Cited by 743
Structural Basis of Transcription Initiation: An RNA Polymerase Holoenzyme-DNA Complex
Murakami, K. S., Masuda, S., Campbell, E. A., Muzzin, O., & Darst, S. A. (2002) · Science · doi:10.1126/science.1069595 · Cited by 641
Structure and mechanism of the RNA polymerase II transcription machinery
Hahn, S. (2004) · Nature Structural & Molecular Biology · doi:10.1038/nsmb763 · Cited by 547
Crystal structure of a TFIIB–TBP–TATA-element ternary complex
Nikolov, D. B., Chen, H., Halay, E. D., Usheva, A. A., Hisatake, K., Lee, D. K., Roeder, R. G., & Burley, S. K. (1995) · Nature · doi:10.1038/377119a0 · Cited by 546
Structural Basis of Transcription Initiation: RNA Polymerase Holoenzyme at 4 Å Resolution
Murakami, K. S., Masuda, S., & Darst, S. A. (2002) · Science · doi:10.1126/science.1069594 · Cited by 541
Complete RNA Polymerase II Elongation Complex Structure and Its Interactions with NTP and TFIIS
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Self-citationStructural Basis of Transcription: An RNA Polymerase II-TFIIB Cocrystal at 4.5 Angstroms
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Evolution of multisubunit RNA polymerases in the three domains of life
Werner, F., & Grohmann, D. (2011) · Nature Reviews Microbiology · doi:10.1038/nrmicro2507 · Cited by 431
Conservation between the RNA Polymerase I, II, and III Transcription Initiation Machineries
Vannini, A., & Cramer, P. (2012) · Molecular Cell · doi:10.1016/j.molcel.2012.01.023 · Cited by 425
Self-citationArchitecture of the RNA polymerase II–TFIIF complex revealed by cross-linking and mass spectrometry
Chen, Z. A., Jawhari, A., Fischer, L., Buchen, C., Tahir, S., Kamenski, T., Rasmussen, M., Lariviere, L., Bukowski-Wills, J.-C., Nilges, M., et al. (2010) · The EMBO Journal · doi:10.1038/emboj.2009.401 · Cited by 391
Self-citationStructural Basis for Promoter −10 Element Recognition by the Bacterial RNA Polymerase σ Subunit
Feklistov, A., & Darst, S. A. (2011) · Cell · doi:10.1016/j.cell.2011.10.041 · Cited by 326
Metadata
- Authors
- Kostrewa, D., Zeller, M. E., Armache, K.-J., Seizl, M., Leike, K., Thomm, M., & Cramer, P.
- Venue
- Nature
- Published
- 2009-10-09
- Volume
- 462
- Issue
- 7271
- Pages
- 323-330
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2159422462
- Cited by
- 317
- References
- 46
- FWCI
- 12.60
- Type
- journal article
- Language
- en
- Open access
- green
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- Kostrewa, D., Zeller, M. E., Armache, K.-J., Seizl, M., Leike, K., Thomm, M., & Cramer, P. (2009). RNA polymerase II–TFIIB structure and mechanism of transcription initiation. Nature. https://doi.org/10.1038/nature08548
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