The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Structural Basis for Promoter −10 Element Recognition by the Bacterial RNA Polymerase σ Subunit
Open accessAbstract
SUMMARY The key step in bacterial promoter opening is recognition of the -10 promoter element (T-12A-11T-10A-9A-8T-7 consensus sequence) by the RNA polymerase σ subunit. We determined crystal structures of σ domain 2 bound to single-stranded DNA bearing -10 element sequences. Extensive interactions occur between the protein and the DNA backbone of every -10 element nucleotide. Base-specific interactions occur primarily with A-11, and T-7, which are flipped out of the single-stranded DNA base-stack and buried deep in protein pockets. The structures, along with biochemical data, support a model where the recognition of the -10 element sequence drives initial promoter opening as the bases of the non-template strand are extruded from the DNA double-helix and captured by σ. These results provide a detailed structural basis for the critical roles of A-11 and T-7 in promoter melting, and reveal important insights into the initiation of transcription bubble formation.
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Metadata
- Authors
- Feklistov, A., & Darst, S. A.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2011-12
- Volume
- 147
- Issue
- 6
- Pages
- 1257-1269
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2111441093
- Cited by
- 326
- References
- 47
- FWCI
- 12.06
- Type
- journal article
- Language
- en
- Open access
- bronze
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