The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy
Open accessAbstract
The translocation step of protein synthesis entails large-scale rearrangements of the ribosome–transfer RNA (tRNA) complex. Here we have followed tRNA movement through the ribosome during translocation by time-resolved single-particle electron cryomicroscopy (cryo-EM). Unbiased computational sorting of cryo-EM images yielded 50 distinct three-dimensional reconstructions, showing the tRNAs in classical, hybrid and various novel intermediate states that provide trajectories and kinetic information about tRNA movement through the ribosome. The structures indicate how tRNA movement is coupled with global and local conformational changes of the ribosome, in particular of the head and body of the small ribosomal subunit, and show that dynamic interactions between tRNAs and ribosomal residues confine the path of the tRNAs through the ribosome. The temperature dependence of ribosome dynamics reveals a surprisingly flat energy landscape of conformational variations at physiological temperature. The ribosome functions as a Brownian machine that couples spontaneous conformational changes driven by thermal energy to directed movement.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
EMAN: Semiautomated Software for High-Resolution Single-Particle Reconstructions
Ludtke, S. J., Baldwin, P. R., & Chiu, W. (1999) · Journal of Structural Biology · doi:10.1006/jsbi.1999.4174 · Cited by 2,391
Cryo-electron microscopy of vitrified specimens
Dubochet, J., Adrian, M., Chang, J.-J., Homo, J.-C., Lepault, J., McDowall, A. W., & Schultz, P. (1988) · Quarterly Reviews of Biophysics · doi:10.1017/s0033583500004297 · Cited by 2,389
Crystal structure of the ribosome at 5.5 Å resolution
Yusupov, M. M., Yusupova, G. Z., Baucom, A., Lieberman, K., Earnest, T. N., Cate, J. H. D., & Noller, H. F. (2001) · Science · doi:10.1126/science.1060089 · Cited by 1,944
Structures of the Bacterial Ribosome at 3.5 Å Resolution
Schuwirth, B. S., Borovinskaya, M. A., Hau, C. W., Zhang, W., Vila-Sanjurjo, A., Holton, J. M., & Cate, J. H. D. (2005) · Science · doi:10.1126/science.1117230 · Cited by 1,418
Thermodynamics and Kinetics of a Brownian Motor
Astumian, R. D. (1997) · Science · doi:10.1126/science.276.5314.917 · Cited by 1,411
Structure of the 70 S Ribosome Complexed with mRNA and tRNA
Selmer, M., Dunham, C. M., Murphy, F. V., Weixlbaumer, A., Petry, S., Kelley, A. C., Weir, J. R., & Ramakrishnan, V. (2006) · Science · doi:10.1126/science.1131127 · Cited by 1,283
A New Generation of the IMAGIC Image Processing System
van Heel, M., Harauz, G., Orlova, E. V., Schmidt, R., & Schatz, M. (1996) · Journal of Structural Biology · doi:10.1006/jsbi.1996.0004 · Cited by 1,228
A ratchet-like inter-subunit reorganization of the ribosome during translocation
Frank, J., & Agrawal, R. K. (2000) · Nature · doi:10.1038/35018597 · Cited by 799
Intermediate states in the movement of transfer RNA in the ribosome
Moazed, D., & Noller, H. F. (1989) · Nature · doi:10.1038/342142a0 · Cited by 738
Controlled environment vitrification system: An improved sample preparation technique
Bellare, J. R., Davis, H. T., Scriven, L. E., & Talmon, Y. (1988) · Journal of Electron Microscopy Technique · doi:10.1002/jemt.1060100111 · Cited by 614
Cited by
RELION: Implementation of a Bayesian approach to cryo-EM structure determination
Scheres, S. H. W. (2012) · Journal of Structural Biology · doi:10.1016/j.jsb.2012.09.006 · Cited by 5,446
The ribosome uses two active mechanisms to unwind messenger RNA during translation
Qu, X., Wen, J.-D., Lancaster, L., Noller, H. F., Bustamante, C., & Tinoco, I. (2011) · Nature · doi:10.1038/nature10126 · Cited by 326
Structure and conformational dynamics of the human spliceosomal Bact complex
Haselbach, D., Komarov, I., Agafonov, D. E., Hartmuth, K., Graf, B., Dybkov, O., Urlaub, H., Kastner, B., Lührmann, R., & Stark, H. (2018) · Cell · doi:10.1016/j.cell.2018.01.010 · Cited by 218
Self-citationVisualizing translation dynamics at atomic detail inside a bacterial cell
Xue, L., Lenz, S., Zimmermann-Kogadeeva, M., Tegunov, D., Cramer, P., Bork, P., Rappsilber, J., & Mahamid, J. (2022) · Nature · doi:10.1038/s41586-022-05255-2 · Cited by 184
Wasserman, M. R., Pulk, A., Zhou, Z., Altman, R. B., Zinder, J. C., Green, K. D., Garneau-Tsodikova, S., Doudna Cate, J. H., & Blanchard, S. C. (2015) · Nature Communications · doi:10.1038/ncomms8896 · Cited by 77
Metadata
- Authors
- Fischer, N., Konevega, A. L., Wintermeyer, W., Rodnina, M. V., & Stark, H.
- Venue
- Nature
- Published
- 2010-07
- Volume
- 466
- Issue
- 7304
- Pages
- 329-333
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2078726019
- Cited by
- 434
- References
- 39
- FWCI
- 17.60
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy. https://app.cochat.ai/papers/10.1038/nature09206
- The paper
- Fischer, N., Konevega, A. L., Wintermeyer, W., Rodnina, M. V., & Stark, H. (2010). Ribosome dynamics and tRNA movement by time-resolved electron cryomicroscopy. Nature. https://doi.org/10.1038/nature09206
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
