The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Genome-wide analysis reveals novel molecular features of mouse recombination hotspots
Open accessAbstract
Meiotic recombination predominantly occurs at discrete genomic loci called recombination hotspots, but the features defining these areas are still largely unknown (reviewed in refs 1–5). To allow a comprehensive analysis of hotspot-associated DNA and chromatin characteristics, we developed a direct molecular approach for mapping meiotic DNA double-strand breaks that initiate recombination. Here we present the genome-wide distribution of recombination initiation sites in the mouse genome. Hotspot centres are mapped with approximately 200-nucleotide precision, which allows analysis of the fine structural details of the preferred recombination sites. We determine that hotspots share a centrally distributed consensus motif, possess a nucleotide skew that changes polarity at the centres of hotspots and have an intrinsic preference to be occupied by a nucleosome. Furthermore, we find that the vast majority of recombination initiation sites in mouse males are associated with testis-specific trimethylation of lysine 4 on histone H3 that is distinct from histone H3 lysine 4 trimethylation marks associated with transcription. The recombination map presented here has been derived from a homogeneous mouse population with a defined genetic background and therefore lends itself to extensive future experimental exploration. We note that the mapping technique developed here does not depend on the availability of genetic markers and hence can be easily adapted to other species with complex genomes. Our findings uncover several fundamental features of mammalian recombination hotspots and underline the power of the new recombination map for future studies of genetic recombination, genome stability and evolution.
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Cites
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PRDM9 Is a Major Determinant of Meiotic Recombination Hotspots in Humans and Mice
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Cited by
Variation in recombination frequency and distribution across eukaryotes: patterns and processes
Stapley, J., Feulner, P. G. D., Johnston, S. E., Santure, A. W., & Smadja, C. M. (2017) · Philosophical Transactions of the Royal Society B: Biological Sciences · doi:10.1098/rstb.2016.0455 · Cited by 507
Genetic recombination is directed away from functional genomic elements in mice
Brick, K., Smagulova, F., Khil, P., Camerini-Otero, R. D., & Petukhova, G. V. (2012) · Nature · doi:10.1038/nature11089 · Cited by 414
Self-citationMAnorm: a robust model for quantitative comparison of ChIP-Seq data sets
Shao, Z., Zhang, Y., Yuan, G.-C., Orkin, S. H., & Waxman, D. J. (2012) · Genome Biology · doi:10.1186/gb-2012-13-3-r16 · Cited by 414
Arabidopsis meiotic crossover hot spots overlap with H2A.Z nucleosomes at gene promoters
Choi, K., Zhao, X., Kelly, K. A., Venn, O., Higgins, J. D., Yelina, N. E., Hardcastle, T. J., Ziolkowski, P. A., Copenhaver, G. P., Franklin, F. C. H., et al. (2013) · Nature Genetics · doi:10.1038/ng.2766 · Cited by 375
Stable recombination hotspots in birds
Singhal, S., Leffler, E. M., Sannareddy, K., Turner, I., Venn, O., Hooper, D. M., Strand, A. I., Li, Q., Raney, B., Balakrishnan, C. N., et al. (2015) · Science · doi:10.1126/science.aad0843 · Cited by 344
Metadata
- Authors
- Smagulova, F., Gregoretti, I. V., Brick, K., Khil, P., Camerini-Otero, R. D., & Petukhova, G. V.
- Venue
- Nature
- Published
- 2011-04
- Volume
- 472
- Issue
- 7343
- Pages
- 375-378
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1963998725
- Cited by
- 340
- References
- 28
- FWCI
- 12.70
- Type
- journal article
- Language
- en
- Open access
- green
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- Smagulova, F., Gregoretti, I. V., Brick, K., Khil, P., Camerini-Otero, R. D., & Petukhova, G. V. (2011). Genome-wide analysis reveals novel molecular features of mouse recombination hotspots. Nature. https://doi.org/10.1038/nature09869
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