Cited by 138Bacterial Genetics and BiotechnologyDNA Repair MechanismsEnzyme Structure and Function

The DOI matches its Crossref record; 65 of the 65 references it cites were found in a registry or an index.

The Mu transpososome structure sheds light on DDE recombinase evolution

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Abstract

Studies of bacteriophage Mu transposition paved the way for understanding retroviral integration and V(D)J recombination as well as many other DNA transposition reactions. Here we report the structure of the Mu transpososome--Mu transposase (MuA) in complex with bacteriophage DNA ends and target DNA--determined from data that extend anisotropically to 5.2 Å, 5.2 Å and 3.7 Å resolution, in conjunction with previously determined structures of individual domains. The highly intertwined structure illustrates why chemical activity depends on formation of the synaptic complex, and reveals that individual domains have different roles when bound to different sites. The structure also provides explanations for the increased stability of the final product complex and for its preferential recognition by the ATP-dependent unfoldase ClpX. Although MuA and many other recombinases share a structurally conserved 'DDE' catalytic domain, comparisons among the limited set of available complex structures indicate that some conserved features, such as catalysis in trans and target DNA bending, arose through convergent evolution because they are important for function.

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Metadata

Authors
Montaño, S. P., Pigli, Y. Z., & Rice, P. A.
Affiliations
University of Chicago (US)
Corresponding author
Rice, Phoebe A.
Venue
Nature
Published
2012-11
Volume
491
Issue
7424
Pages
413-417
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W2034741116
Cited by
137
References
65
Citations by year
2026: 4 · 2025: 4 · 2024: 4 · 2023: 12 · 2022: 7 · 2021: 13 · 2020: 9 · 2019: 9 · 2018: 6 · 2017: 12 · 2016: 8 · 2015: 24 · 2014: 13 · 2013: 11 · 2012: 1
FWCI
9.40
Type
journal article
Language
en
Open access
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Funders
National Institute of General Medical Sciences (GM086826, R01GM086826)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
Keywords
bacteriophage Mu, transpososome, DNA transposition, synaptic complex, transposon ends, target DNA, convergent evolution, protein-DNA complex structure
MeSH terms
DNA, Viral, Models, Molecular, Bacteriophage mu, Protein Binding, Protein Structure, Tertiary, Evolution, Molecular, Transposases, Recombinases

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