The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
PAAR-repeat proteins sharpen and diversify the type VI secretion system spike
Open accessAbstract
The bacterial type VI secretion system (T6SS) is a large multicomponent, dynamic macromolecular machine that has an important role in the ecology of many Gram-negative bacteria. T6SS is responsible for translocation of a wide range of toxic effector molecules, allowing predatory cells to kill both prokaryotic as well as eukaryotic prey cells. The T6SS organelle is functionally analogous to contractile tails of bacteriophages and is thought to attack cells by initially penetrating them with a trimeric protein complex called the VgrG spike. Neither the exact protein composition of the T6SS organelle nor the mechanisms of effector selection and delivery are known. Here we report that proteins from the PAAR (proline-alanine-alanine-arginine) repeat superfamily form a sharp conical extension on the VgrG spike, which is further involved in attaching effector domains to the spike. The crystal structures of two PAAR-repeat proteins bound to VgrG-like partners show that these proteins sharpen the tip of the T6SS spike complex. We demonstrate that PAAR proteins are essential for T6SS-mediated secretion and target cell killing by Vibrio cholerae and Acinetobacter baylyi. Our results indicate a new model of the T6SS organelle in which the VgrG–PAAR spike complex is decorated with multiple effectors that are delivered simultaneously into target cells in a single contraction-driven translocation event.
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Secretion systems in Gram-negative bacteria: Structural and mechanistic insights
Costa, T. R. D., Felisberto-Rodrigues, C., Meir, A., Prevost, M. S., Redzej, A., Trokter, M., & Waksman, G. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3456 · Cited by 1,132
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The Type VI secretion system: a versatile bacterial weapon
Coulthurst, S. (2019) · Microbiology · doi:10.1099/mic.0.000789 · Cited by 346
Structure of the T4 baseplate and its function in triggering sheath contraction
Taylor, N. M. I., Prokhorov, N. S., Guerrero-Ferreira, R. C., Shneider, M. M., Browning, C., Goldie, K. N., Stahlberg, H., & Leiman, P. G. (2016) · Nature · doi:10.1038/nature17971 · Cited by 299
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Metadata
- Authors
- Shneider, M. M., Buth, S. A., Ho, B. T., Basler, M., Mekalanos, J. J., & Leiman, P. G.
- Venue
- Nature
- Published
- 2013-08
- Volume
- 500
- Issue
- 7462
- Pages
- 350-353
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2128007785
- Cited by
- 526
- References
- 5
- FWCI
- 23.06
- Type
- journal article
- Language
- en
- Open access
- green
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