Secretion systems in Gram-negative bacteria: Structural and mechanistic insights
Abstract
Bacteria have evolved a remarkable array of sophisticated nanomachines to export various virulence factors across the bacterial cell envelope. In recent years, considerable progress has been made towards elucidating the structural and molecular mechanisms of the six secretion systems (types I-VI) of Gram-negative bacteria, the unique mycobacterial type VII secretion system, the chaperone-usher pathway and the curli secretion machinery. These advances have greatly enhanced our understanding of the complex mechanisms that these macromolecular structures use to deliver proteins and DNA into the extracellular environment or into target cells. In this Review, we explore the structural and mechanistic relationships between these single- and double-membrane-embedded systems, and we briefly discuss how this knowledge can be exploited for the development of new antimicrobial strategies.
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E. coli exploits three main pathways for protein translocation to the periplasm: the SecB-dependent, the SRP-mediated and the twin-arginine transport pathways.
“Like other Gram-negative bacteria, E. coli exploits three main pathways for protein translocation to the periplasm: the SecB-dependent, the SRP-mediated and the twin-arginine transport (TAT) translocation pathways [131,132,133].”
From Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments (2020) · cites this paper as [132]
Cites
Take five — Type VII secretion systems of Mycobacteria
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Protein export through the bacterial Sec pathway
Tsirigotaki, A., De Geyter, J., Šoštaric´, N., Economou, A., & Karamanou, S. (2017) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2016.161 · Cited by 447 · see citing works
ESX secretion systems: mycobacterial evolution to counter host immunity
Gröschel, M. I., Sayes, F., Simeone, R., Majlessi, L., & Brosch, R. (2016) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2016.131 · Cited by 382 · see citing works
The Type IX Secretion System (T9SS): Highlights and Recent Insights into Its Structure and Function
Lasica, A. M., Ksiazek, M., Madej, M., & Potempa, J. (2017) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2017.00215 · Cited by 312 · see citing works
Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments
Sandomenico, A., Sivaccumar, J. P., & Ruvo, M. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21176324 · Cited by 134 · see citing works
Structure of an endogenous mycobacterial MCE lipid transporter
Chen, J., Fruhauf, A., Fan, C., Ponce, J., Ueberheide, B., Bhabha, G., & Ekiert, D. C. (2023) · Nature · doi:10.1038/s41586-023-06366-0 · Cited by 69 · see citing works
Two Accessory Proteins Govern MmpL3 Mycolic Acid Transport in Mycobacteria
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Monitoring substrate enables real-time regulation of a protein localization pathway.
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- Authors
- Costa, T. R. D., Felisberto-Rodrigues, C., Meir, A., Prevost, M. S., Redzej, A., Trokter, M., & Waksman, G.
- Affiliations
- Institute of Structural and Molecular Biology (GB)
- Corresponding author
- Waksman, Gabriel
- Venue
- Nature Reviews Microbiology
- Published
- 2015-05-15
- Volume
- 13
- Issue
- 6
- Pages
- 343-359
- ISSN
- 1740-1526 (ISSN-L)
- 1740-1534
- OpenAlex
- W2130599662
- Cited by
- 1,132
- References
- 1
- Citations by year
- 2026: 77 · 2025: 113 · 2024: 91 · 2023: 108 · 2022: 98 · 2021: 100 · 2020: 98 · 2019: 103 · 2018: 105 · 2017: 129 · 2016: 93 · 2015: 17
- FWCI
- 14.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Reviews Microbiology (publishedVersion)
- PubMed (publishedVersion)
- BIROn (Birkbeck, University of London) (submittedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Wellcome Trust (098302/Z/12/Z, 098302)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- bacterial secretion systems, type III secretion, type IV secretion system, type VII secretion system, chaperone-usher pathway, Gram-negative bacteria, mycobacteria, secretion mechanism, antibiotic targets
- MeSH terms
- Anti-Bacterial Agents, Cell Membrane, Gram-Negative Bacteria, Fimbriae, Bacterial, Gram-Negative Bacterial Infections, Molecular Chaperones, Virulence Factors, Bacterial Secretion Systems
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