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Cited by 891Plant Parasitism and ResistancePlant Molecular Biology ResearchPlant biochemistry and biosynthesis

D14–SCFD3-dependent degradation of D53 regulates strigolactone signalling

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Zhou, Feng, Lin, Qibing, Zhu, Lihong, Ren, Yulong, Zhou, Kunneng, Shabek, Nitzan, Wu, Fuqing, Mao, Haibin +27 more·2013·Nature
DOI 10.1038/nature12878PMC PMC4096652
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Abstract

Strigolactones (SLs), a newly discovered class of carotenoid-derived phytohormones, are essential for developmental processes that shape plant architecture and interactions with parasitic weeds and symbiotic arbuscular mycorrhizal fungi. Despite the rapid progress in elucidating the SL biosynthetic pathway, the perception and signalling mechanisms of SL remain poorly understood. Here we show that DWARF 53 (D53) acts as a repressor of SL signalling and that SLs induce its degradation. We find that the rice (Oryza sativa) d53 mutant, which produces an exaggerated number of tillers compared to wild-type plants, is caused by a gain-of-function mutation and is insensitive to exogenous SL treatment. The D53 gene product shares predicted features with the class I Clp ATPase proteins and can form a complex with the α/β hydrolase protein DWARF 14 (D14) and the F-box protein DWARF 3 (D3), two previously identified signalling components potentially responsible for SL perception. We demonstrate that, in a D14- and D3-dependent manner, SLs induce D53 degradation by the proteasome and abrogate its activity in promoting axillary bud outgrowth. Our combined genetic and biochemical data reveal that D53 acts as a repressor of the SL signalling pathway, whose hormone-induced degradation represents a key molecular link between SL perception and responses.

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  • Rice D53 interacts with D3, the rice ortholog of MAX2, and d3 mutants are suppressed by knockdowns of D53.

    “Moreover, rice D53 interacts with D3, which is the rice ortholog of MAX2, and d3 mutants are suppressed by knockdowns of D53 (Jiang et al., 2013; Zhou et al., 2013).”

    From Shaping plant architecture (2015) · cites this paper as Zhou et al., 2013

    In “GR24 promotes D53 protein degradation” (page unavailable), the paper reports that D53 knockdown restored the d3 mutant phenotype to nearly wild-type levels. In “SLs promote D53–D14 and D14–D3 interaction” (page unavailable), it reports D53–D14 and D14–D3 interactions and suggests complex formation among all three, but does not establish a direct D53–D3 interaction. The Discussion identifies D3 as the rice ortholog of MAX2. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • Dominant gain of function mutations in D53 prevent SL-mediated degradation of the protein and shut off SL signaling.

    “Dominant gain of function mutations in D53 prevent SL-mediated degradation of the protein and shut off SL signaling.”

    From Shaping plant architecture (2015) · cites this paper as Zhou et al., 2013

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Authors
Zhou, F., Lin, Q., Zhu, L., Ren, Y., Zhou, K., Shabek, N., Wu, F., Mao, H., Dong, W., Gan, L., Ma, W., Gao, H., Chen, J., Yang, C., Wang, D., Tan, J., Zhang, X., Guo, X., Wang, J., Jiang, L., Liu, X., Chen, W., Chu, J., Yan, C., Ueno, K., Ito, S., Asami, T., Cheng, Z., Wang, J., Lei, C., Zhai, H., Wu, C., Wang, H., Zheng, N., & Wan, J.
Affiliations
  • Nanjing Agricultural University (CN)
  • Chinese Academy of Agricultural Sciences (CN)
  • Howard Hughes Medical Institute (US)
  • Chinese Academy of Sciences (CN)
  • The University of Tokyo (JP)
Corresponding author
  • Wang, Haiyang
  • Zheng, Ning
  • Wan, Jianmin
ORCID
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2013-12-11
Volume
504
Issue
7480
Pages
406-410
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/nature12878
PMC
PMC4096652
OpenAlex
W1978285405
Cited by
891
References
4
Citations by year
2026: 65 · 2025: 83 · 2024: 88 · 2023: 78 · 2022: 83 · 2021: 64 · 2020: 87 · 2019: 77 · 2018: 55 · 2017: 63 · 2016: 53 · 2015: 55 · 2014: 38 · 2013: 1 · 2012: 1
FWCI
59.18
Type
journal article
Language
en
Update notices
correction (2016-01-13)
Open access
Licence
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Cancer Institute (R01CA107134)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
strigolactone signalling, protein degradation, ubiquitination, proteasome, F-box protein, rice, axillary bud outgrowth, tillering, plant growth regulation, arbuscular mycorrhizal fungi, parasitic weeds, plant architecture
MeSH terms
Amino Acid Sequence, Cloning, Molecular, Lactones, Molecular Sequence Data, Mutation, Phenotype, Plant Growth Regulators, Plant Proteins, Protein Binding, Oryza, Signal Transduction, Gene Expression Regulation, Plant, SKP Cullin F-Box Protein Ligases, Proteasome Endopeptidase Complex, Proteolysis

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