Cited by 378Plant Parasitism and ResistancePlant biochemistry and biosynthesisPlant Molecular Biology Research

Molecular mechanism of strigolactone perception by DWARF14

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Nakamura, Hidemitsu, Xue, You-Lin, Miyakawa, Takuya, Hou, Feng, Qin, Hui-Min, Fukui, Kosuke, Shi, Xuan, Ito, Emi +8 more·2013·Nature Communications

Abstract

Strigolactones (SLs) are phytohormones that inhibit shoot branching and function in the rhizospheric communication with symbiotic fungi and parasitic weeds. An α/β-hydrolase protein, DWARF14 (D14), has been recognized to be an essential component of plant SL signalling, although its precise function remains unknown. Here we present the SL-dependent interaction of D14 with a gibberellin signalling repressor SLR1 and a possible mechanism of phytohormone perception in D14-mediated SL signalling. D14 functions as a cleavage enzyme of SLs, and the cleavage reaction induces the interaction with SLR1. The crystal structure of D14 shows that 5-hydroxy-3-methylbutenolide (D-OH), which is a reaction product of SLs, is trapped in the catalytic cavity of D14 to form an altered surface. The D14 residues recognizing D-OH are critical for the SL-dependent D14-SLR1 interaction. These results provide new insight into crosstalk between gibberellin and SL signalling pathways.

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Crossref · 10.1038/ncomms3613

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  • Three different candidate repressors for the strigolactone signaling pathway have been identified: DELLA proteins, BES1, and D53.

    “Indeed, three different candidate repressors for the strigoalactone signaling pathway have been identified: DELLA proteins (Nakamura et al., 2013), BES1 (Wang et al., 2013), and D53 in rice (Jiang et al., 2013; Zhou et al., 2013).”

    From Shaping plant architecture (2015) · cites this paper as Nakamura et al., 2013

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Authors
Nakamura, H., Xue, Y.-L., Miyakawa, T., Hou, F., Qin, H.-M., Fukui, K., Shi, X., Ito, E., Ito, S., Park, S.-H., Miyauchi, Y., Asano, A., Totsuka, N., Ueda, T., Tanokura, M., & Asami, T.
Affiliations
  • The University of Tokyo (JP)
  • Japan Science and Technology Agency (JP)
Corresponding author
Asami, Tadao
Venue
Nature Communications
Published
2013-10-17
Volume
4
Issue
1
Pages
2613-2613
ISSN
2041-1723 (ISSN-L)
OpenAlex
W2013437585
Cited by
378
References
55
Citations by year
2026: 18 · 2025: 15 · 2024: 23 · 2023: 31 · 2022: 29 · 2021: 28 · 2020: 31 · 2019: 32 · 2018: 32 · 2017: 39 · 2016: 39 · 2015: 22 · 2014: 35 · 2013: 4
FWCI
40.18
Type
journal article
Language
en
Open access
Locations
  • Nature Communications (publishedVersion)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Funders
  • Ministry of Education, Culture, Sports, Science and Technology (CREST)
  • Shimane University
  • University of Tokyo
  • Japan Society for the Promotion of Science (23228003, 20001009, 23248018, CREST, 24370019, 24114003)
  • Japan Science and Technology Agency (CREST)
  • Core Research for Evolutional Science and Technology
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Parasitism and Resistance
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
strigolactone perception, DWARF14, SLR1, DELLA proteins, shoot branching, plant hormone signaling, protein crystal structure, alpha/beta hydrolase, plant-fungal symbiosis, parasitic weeds
MeSH terms
Escherichia coli, Gibberellins, Hydrolases, Plant Growth Regulators, Plant Proteins, Recombinant Proteins, Oryza, 4-Butyrolactone, Signal Transduction, Crystallography, X-Ray, Gene Expression Regulation, Plant, Two-Hybrid System Techniques, Plants, Genetically Modified, Rhizosphere

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