The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
A three-dimensional human neural cell culture model of Alzheimer’s disease
Open accessAbstract
Alzheimer's disease is the most common form of dementia, characterized by two pathological hallmarks: amyloid-β plaques and neurofibrillary tangles. The amyloid hypothesis of Alzheimer's disease posits that the excessive accumulation of amyloid-β peptide leads to neurofibrillary tangles composed of aggregated hyperphosphorylated tau. However, to date, no single disease model has serially linked these two pathological events using human neuronal cells. Mouse models with familial Alzheimer's disease (FAD) mutations exhibit amyloid-β-induced synaptic and memory deficits but they do not fully recapitulate other key pathological events of Alzheimer's disease, including distinct neurofibrillary tangle pathology. Human neurons derived from Alzheimer's disease patients have shown elevated levels of toxic amyloid-β species and phosphorylated tau but did not demonstrate amyloid-β plaques or neurofibrillary tangles. Here we report that FAD mutations in β-amyloid precursor protein and presenilin 1 are able to induce robust extracellular deposition of amyloid-β, including amyloid-β plaques, in a human neural stem-cell-derived three-dimensional (3D) culture system. More importantly, the 3D-differentiated neuronal cells expressing FAD mutations exhibited high levels of detergent-resistant, silver-positive aggregates of phosphorylated tau in the soma and neurites, as well as filamentous tau, as detected by immunoelectron microscopy. Inhibition of amyloid-β generation with β- or γ-secretase inhibitors not only decreased amyloid-β pathology, but also attenuated tauopathy. We also found that glycogen synthase kinase 3 (GSK3) regulated amyloid-β-mediated tau phosphorylation. We have successfully recapitulated amyloid-β and tau pathology in a single 3D human neural cell culture system. Our unique strategy for recapitulating Alzheimer's disease pathology in a 3D neural cell culture model should also serve to facilitate the development of more precise human neural cell models of other neurodegenerative disorders.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Beading or blebbing in neurites is a marker of dystrophic neurites in the AD brain (3, 42, 43).”
From Modeling late-onset Alzheimer’s disease neuropathology via direct neuronal reprogramming (2024) · cites this paper as [3]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“In AD, tau protein becomes hyperphosphorylated, resulting in tau dissociating from microtubules and forming insoluble tau aggregates in the neurites and cell bodies (3, 37).”
From Modeling late-onset Alzheimer’s disease neuropathology via direct neuronal reprogramming (2024) · cites this paper as [3]
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“In iPSC-derived neurons from both FAD- and SAD-patients, pTau aberrantly accumulates at early time points (Choi et al., 2014, Israel et al., 2012, Moore et al., 2015, Muratore et al., 2014, Ochalek et al., 2017, Shi et al., 2012).”
From Cholesterol Metabolism Is a Druggable Axis that Independently Regulates Tau and Amyloid-β in iPSC-Derived Alzheimer’s Disease Neurons (2019) · cites this paper as Choi et al., 2014
Experimental — accuracy not yet measuredThe cited source says what the claim attributes to it.
In the citing paper
“Modeling these pathological hallmarks has largely relied on transgenic cellular and animal models harboring genetic mutations linked to ADAD (3–5).”
From Modeling late-onset Alzheimer’s disease neuropathology via direct neuronal reprogramming (2024) · cites this paper as [3]
The cited source says what the claim attributes to it.
In the citing paper
“As for human neurons, previous studies focused on overexpressing mutant APP and Presenilin in REN stem cells grown in a 3D culture (3), which differs from patient-specific LOAD model that relies on the endogenous recapitulation of AD-associated phenotypes as provided in this study.”
From Modeling late-onset Alzheimer’s disease neuropathology via direct neuronal reprogramming (2024) · cites this paper as [3]
In the citing paper
“one study revealed that familial AD (FAD) mutations in β-amyloid precursor protein and presenilin 1 induced robust extracellular deposition of amyloid-β in a human neural stem-cell-derived 3D culture system85.”
From The use of brain organoids to investigate neural development and disease (2017) · cites this paper as [85]
In the citing paper
“the 3D-differentiated neuronal cells expressing FAD mutations exhibited aggregates of phosphorylated tau as well as filamentous tau.”
From The use of brain organoids to investigate neural development and disease (2017) · cites this paper as [85]
In the citing paper
“A 3D in vitro culture system consisting of human neurons differentiated from induced pluripotent stem cells overexpressing human APP containing FAD mutations generates robust Aβ42 production and amyloid deposition. Importantly, this model also develops elevated phospho-tau levels and fibrillary tau aggregates, suggesting that elevated Aβ levels alone are sufficient to drive tau pathology in human”
From Alzheimer Disease: An Update on Pathobiology and Treatment Strategies (2019) · cites this paper as Choi et al., 2014
In the citing paper
“It was recently shown that development of a 3D culture of human neural stem cells transfected with APPK670N/M671L/V717I and PS1ΔE9 in Matrigel scaffolding resulted in the extracellular aggregation of Aβ into plaques and the intracellular aggregation of tau in dystrophic neurites and the cell soma [23, 77].”
From Alzheimer's disease: experimental models and reality (2017) · cites this paper as [23]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The Amyloid Hypothesis of Alzheimer’s Disease: Progress and Problems on the Road to Therapeutics
Hardy, J., & Selkoe, D. J. (2002) · Science · doi:10.1126/science.1072994 · Cited by 13,864
Cerebral organoids model human brain development and microcephaly
Lancaster, M. A., Renner, M., Martin, C.-A., Wenzel, D., Bicknell, L. S., Hurles, M. E., Homfray, T., Penninger, J. M., Jackson, A. P., & Knoblich, J. A. (2013) · Nature · doi:10.1038/nature12517 · Cited by 5,372
Alzheimer's Disease Is a Synaptic Failure
Selkoe, D. J. (2002) · Science · doi:10.1126/science.1074069 · Cited by 4,420
Twenty years of the Alzheimer's disease amyloid hypothesis: a genetic perspective
Tanzi, R. E., & Bertram, L. (2005) · Cell · doi:10.1016/j.cell.2005.02.008 · Cited by 1,849
Self-citationProbing sporadic and familial Alzheimer’s disease using induced pluripotent stem cells
Israel, M. A., Yuan, S. H., Bardy, C., Reyna, S. M., Mu, Y., Herrera, C., Hefferan, M. P., Van Gorp, S., Nazor, K. L., Boscolo, F. S., et al. (2012) · Nature · doi:10.1038/nature10821 · Cited by 1,253
GSK-3α regulates production of Alzheimer's disease amyloid-β peptides
Phiel, C. J., Wilson, C., Lee, V. M. ‐., & Klein, P. S. (2003) · Nature · doi:10.1038/nature01640 · Cited by 1,211
Kondo, T., Asai, M., Tsukita, K., Kutoku, Y., Ohsawa, Y., Sunada, Y., Imamura, K., Egawa, N., Yahata, N., Okita, K., et al. (2013) · Cell Stem Cell · doi:10.1016/j.stem.2013.01.009 · Cited by 741
Modeling familial Alzheimer's disease with induced pluripotent stem cells
Yagi, T., Ito, D., Okada, Y., Akamatsu, W., Nihei, Y., Yoshizaki, T., Yamanaka, S., Okano, H., & Suzuki, N. (2011) · Human Molecular Genetics · doi:10.1093/hmg/ddr394 · Cited by 599
Cohen, R. M., Rezai-Zadeh, K. P., Weitz, T. M., Rentsendorj, A., Gate, D., Spivak, I. E., Bholat, Y., Vasilevko, V., Glabe, C. G., Breunig, J. J., et al. (2013) · Journal of Neuroscience · doi:10.1523/jneurosci.3672-12.2013 · Cited by 555
Muratore, C. R., Rice, H. C., Srikanth, P., Callahan, D. G., Shin, T., Benjamin, L. N. P., Walsh, D. M., Selkoe, D. J., & Young-Pearse, T. L. (2014) · Human Molecular Genetics · doi:10.1093/hmg/ddu064 · Cited by 379
Flagged first, then by citations
Research works printed but not linked: 1
- Higuchi, M., Trojanowski, J. Q. & Lee, V. M.-Y. Tau protein and tauopathy. Neuropsychopharmacology: The Fifth Generation of Progress (eds Davis, K. L., Charney, D., Coyle, J. T. & Nemeroff, C. ) 1339–1354 (Lippincott, Williams & Wilkins, 2002)
Cited by
Sorted by citations
Items 901–998 of 998
Bal‐Price, A. (2018) · ALTEX · doi:10.14573/altex.1712081s · Cited by 4
Minimalistic in vitro systems for investigating tau pathology
Hallinan, G. I., Pitera, A. P., Patel, P., West, J. J., & Deinhardt, K. (2018) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2018.09.032 · Cited by 4
[Study on the measurement of ambient ammonia in urban area based on open-path DOAS technique].
Wang, J., Xie, P.-H., Qin, M., Ling, L.-Y., Ye, C.-L., Liu, J., & Liu, W. (2012) · PubMed · Cited by 4
Papadimitriou, C., Coşacak, M. İ., Mashkaryan, V., Çelikkaya, H., Bray, L. J., Bhattarai, P., Hollak, H., Chen, X., He, S., Antos, C. L., et al. (2017) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/225243 · Cited by 4
Lovejoy, C. E. J., Alatza, A., Arber, C., Galasso, G., Bradshaw, T. Y., Verheyen, A., Lashley, T., Révész, T., Hardy, J., Karch, C. M. M., et al. (2020) · doi:10.21203/rs.3.rs-37620/v1 · Cited by 3
Cuní‐López, C., Quek, H., Oikari, L. E., Stewart, R., Nguyen, T. H., Sun, Y., Guo, C. C., Lupton, M. K., & White, A. R. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.03.17.435758 · Cited by 4
OECD (2015) · OECD science, technology and industry policy papers · doi:10.1787/5js1t57jts44-en · Cited by 5
Inorganic Nanoparticles-based Drug Delivery Systems for Neurodegenerative Diseases Therapy
Bagherpour, I., Mozafari, M. R., & Naghib, S. M. (2025) · Current Pharmaceutical Design · doi:10.2174/0113816128352935250116064725 · Cited by 5
d’Orsi, G., Calò, E., Claudio, M. T. D., Ferro, C. P., Costantino, U., Perrone, E., & Latino, R. R. (2026) · Epilepsia Open · doi:10.1002/epi4.70277 · Cited by 3
(2017) · Alternative and Complementary Therapies · doi:10.1089/act.2017.29136.ret · Cited by 2
New dimensions in Alzheimer's modelling
Whalley, K. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4494 · Cited by 3
A Synergistic Engineering Approach to Build Human Brain Spheroids
von Maydell, D. K., & Jorfi, M. (2020) · Methods in molecular biology · doi:10.1007/978-1-0716-1174-6_11 · Cited by 3
Pericytes and the Neurovascular Unit: The Critical Nexus of Alzheimer Disease Pathogenesis?
Cercy, S. P. (2021) · Exploratory Research and Hypothesis in Medicine · doi:10.14218/erhm.2020.00062 · Cited by 4
Sharlow, E. R., Köseoğlu, M. M., Bloom, G. S., & Lazo, J. S. (2019) · Assay and Drug Development Technologies · doi:10.1089/adt.2019.914 · Cited by 3
Zhang, C. M., Inamdar, S. M., Swaminathan, S., Marenda, D. R., & Saunders, A. J. (2022) · Frontiers in Neuroscience · doi:10.3389/fnins.2022.821059 · Cited by 3
Self-citationSantiago-Lopez, A. J., Hovell, C. M., Lee, H., & Kim, Y. (2015) · Biosystems & biorobotics · doi:10.1007/978-3-319-21813-7_13 · Cited by 3
Self-citationInhibition of Tau Aggregation as a Basis for Treatment and Prevention of Alzheimer’s Disease
Wischik, C. M., Storey, J. M. D., Wischik, D., & Harrington, C. R. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-802173-6.00015-0 · Cited by 3
Reprogramming Fibroblasts to Neural Stem Cells by Overexpression of the Transcription Factor Ptf1a
Jin, K., Zou, M., Xiao, D., & Xiang, M. (2020) · Methods in molecular biology · doi:10.1007/978-1-0716-0301-7_15 · Cited by 3
Alzheimer’s Disease: Causes, Mechanisms, and Steps Toward Prevention
Choi, S. H., & Tanzi, R. E. (2020) · Oxford University Press eBooks · doi:10.1093/oxfordhb/9780190069162.013.18 · Cited by 3
Self-citationLive-Cell Imaging of Single Neurotrophin Receptor Molecules on Human Neurons in Alzheimer’s Disease
Barabás, K., Kobolák, J., Godó, S., Ernszt, D., Kecskés, M., Varga, C., Kardos, J., Jánosi, T. Z., Fujiwara, T. K., Kusumi, A., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222413260 · Cited by 3
Shahbaz, P., Haq, S. U., & Boz, İ. (2022) · IntechOpen eBooks · doi:10.5772/intechopen.106002 · Cited by 3
Namchaiw, P., Bunreangsri, P., Eiamcharoen, P., Eiamboonsert, S., & Poo-arporn, R. P. (2022) · PLoS ONE · doi:10.1371/journal.pone.0273458 · Cited by 3
Aksnes, M., Tiiman, A., Edwin, T. H., Terenius, L. Y., Bogdanović, N., Vukojević, V. B., & Knapskog, A. (2021) · Frontiers in Aging Neuroscience · doi:10.3389/fnagi.2020.608628 · Cited by 3
Shakeri, A. (2017) · UWSpace (University of Waterloo) · Cited by 2
Eimer, W. A., Kumar, D. K. V., Shanmugam, N. K. N., Washicosky, K. J., Rodriguez, A. S., György, B., Breakefield, X. O., Tanzi, R. E., & Moir, R. D. (2018) · SSRN Electronic Journal · doi:10.2139/ssrn.3155923 · Cited by 3
Self-citationCuní‐López, C., Quek, H., Oikari, L. E., Stewart, R., Nguyen, T. H., Sun, Y., Guo, C. C., Lupton, M. K., & White, A. R. (2021) · Research Square · doi:10.21203/rs.3.rs-571254/v1 · Cited by 3
Wasielewska, J. M., Bajracharya, R., Johnston, R. L., Chaves, J. C. S., Pébay, A., Oikari, L. E., Götz, J., Nisbet, R. M., & White, A. R. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.10.24.563706 · Cited by 2
Sindi, G., Ismael, S., Uddin, R., Slepchenko, K. G., Colvin, R. A., & Lee, D. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.06.02.597022 · Cited by 3
Experimental Models of Alzheimer’s Disease
Ercan, M. (2024) · doi:10.69860/nobel.9786053359166.7 · Cited by 3
Chávez-López, L. M., Silvestre-Martínez, J. H., Lugo-Ibarra, K. C., & Castro-Ceseña, A. B. (2025) · Brain Research · doi:10.1016/j.brainres.2025.149718 · Cited by 3
Unveiling roles of NGLY1 in cellular homeostasis and related diseases
Zhan, C., Zhao, X., Wei, Y., Yang, R., & Yan, N. (2025) · Cellular Signalling · doi:10.1016/j.cellsig.2025.111971 · Cited by 2
Yu, X., Cai, H., He, C., Ouyang, Z., Li, Y., & Chen, L. (2025) · Scientific Reports · doi:10.1038/s41598-025-15242-y · Cited by 2
Curcumin Delivery in Regenerative Medicine for Alzheimer’s Disease
Jafarisavari, Z., Hasanzadeh, E., Tayebi, L., & Asadpour, S. (2025) · Regenerative Engineering and Translational Medicine · doi:10.1007/s40883-025-00482-1 · Cited by 2
Amyloid‐β Seeds in Alzheimer's Disease: Research Challenges and Implications
Beschörner, N., Xu, Y., Jucker, M., & Ruiz‐Riquelme, A. (2025) · Journal of Neurochemistry · doi:10.1111/jnc.70267 · Cited by 2
Gentry, E., Islam, M. T., Xue, H., Cao, K., & Nemes, P. (2025) · Molecular & Cellular Proteomics · doi:10.1016/j.mcpro.2025.101490 · Cited by 2
Delbono, O., Wang, Z., & Messi, M. L. (2025) · Ageing Research Reviews · doi:10.1016/j.arr.2025.102999 · Cited by 3
Kaushik, A., Mishra, D. T. P., Solanki, R., & Bhatia, D. D. (2026) · ACS Pharmacology & Translational Science · doi:10.1021/acsptsci.5c00630 · Cited by 2
Yang, L., Sheng, J., Qi, S., Yin, Z., Chan, M., Cao, Y., Zhao, H., Wan, Z., Chan, B., Ahn, J. Y., et al. (2026) · Molecular Neurodegeneration · doi:10.1186/s13024-026-00943-3 · Cited by 2
RNA-based therapeutics for Alzheimer’s disease and related tauopathies: challenges and opportunities
Rajbanshi, B., Brentari, I., Denti, M. A., Cummings, J. L., & Guruacharya, A. (2026) · The Journal of Prevention of Alzheimer s Disease · doi:10.1016/j.tjpad.2026.100585 · Cited by 2
Toklucu, E. S., Shen, S., Wang, C., & Zhang, C. (2026) · Biomedicines · doi:10.3390/biomedicines14051155 · Cited by 2
Self-citationViable human brain microvessels for the study of aging and neurodegenerative diseases
Damodarasamy, M., Khaing, Z. Z., Hyde, J. E., Keene, C. D., Bentov, I., Banks, W. A., & Reed, M. J. (2021) · Microvascular Research · doi:10.1016/j.mvr.2021.104282 · Cited by 2
Development of early biomarkers of Alzheimer's disease: A precision medicine perspective
Sarkar, S., Das, N., & Sambamurti, K. (2022) · Elsevier eBooks · doi:10.1016/b978-0-12-824010-6.00006-x · Cited by 2
Dreidimensionale Zellkultursysteme
Schmitz, S. (2020) · Der Experimentator · doi:10.1007/978-3-662-58951-9_10 · Cited by 2
Alzheimer Hastalığı’nda In Vivo ve In Vitro Modeller
Aru, B., DEVELİOĞLU, A., Gürel, G., & Demirel, G. Y. (2023) · Türkiye Sağlık Enstitüleri Başkanlığı Dergisi · doi:10.54537/tusebdergisi.1213712 · Cited by 3
Evaluation of Cell-Specific Alterations in Alzheimer’s Disease and Relevance of In Vitro Models
Guido, G., Mangano, K., Tancheva, L. P., Kalfin, R. E., Leone, G. M., Saraceno, A., Fagone, P., Nicoletti, F., & Petralia, M. C. (2023) · Genes · doi:10.3390/genes14122187 · Cited by 2
Volloch, V., & Rits‐Volloch, S. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25189913 · Cited by 3
Tissue-Engineered Three-Dimensional Platforms for Disease Modeling and Therapeutic Development
Wheeler, E. E., & Leach, J. K. (2024) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2024.0212 · Cited by 3
Alzheimer’s Disease from Modeling to Mechanism Research
Sun, X., & Zhang, W. (2024) · Advances in neurobiology · doi:10.1007/978-3-031-69188-1_7 · Cited by 2
Volloch, V., & Rits‐Volloch, S. (2025) · Genes · doi:10.3390/genes16010046 · Cited by 3
Luo, J., Tang, Q., Lin, T., Liu, J., Wu, Z., Zhang, X., Zhang, X., Jiang, J., & Wang, Y. (2025) · Scientific Reports · doi:10.1038/s41598-025-02847-6 · Cited by 2
Clément, B., Pfister, C., Kurer, T., Baalbaki, N., Paxtian-Treviño, M. E., Sim, J., Labouesse, C., Deshmukh, D., Hengsteler, J., Lehmann, J., et al. (2026) · Advanced Healthcare Materials · doi:10.1002/adhm.71655 · Cited by 1
Misfolded Protein Linked Strategies Toward Biomarker Development for Neurodegenerative Diseases
Kumar, S., Narayanan, K., Mishra, S., Padmanabhan, P., Radda, G. K., & Gulyás, B. Z. (2018) · Molecular Neurobiology · doi:10.1007/s12035-018-1232-4 · Cited by 2
Neural tissue microphysiological systems in the era of patient-derived pluripotent stem cells
Mungenast, A. E., Aron, R., White, J. D., Tardiff, D. F., & Raja, W. K. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-813671-3.00009-8 · Cited by 2
Liu, S., Zhao, Y., Su, X., Zhou, C., Yang, P., Lin, Q., Li, S., Tan, H., Wang, Q., Wang, C., et al. (2020) · Stem Cells International · doi:10.1155/2020/8897494 · Cited by 2
Engineered models for studying blood-brain-barrier-associated brain physiology and pathology
Kim, H. N. (2021) · Organoid · doi:10.51335/organoid.2021.1.e10 · Cited by 2
Metabolic Profiles Delineate the Effect of Shengmai San on Alzheimer’s Disease in Rats
Sun, H. J., Han, Y., Zhang, A., & Wang, X. (2015) · Elsevier eBooks · doi:10.1016/b978-0-12-803117-9.00023-8 · Cited by 2
New dimensions in Alzheimer's modelling
Whalley, K. (2014) · Nature reviews. Neuroscience · doi:10.1038/nrn3860 · Cited by 2
Basic aspects in selecting a suitable transgenic rodent model for Alzheimer's disease.
Balmus, I. M., Ciobica, A. S., Negură, A., Negură, L., & Anton, E. (2015) · PubMed · Cited by 2
Benefits of Strategic Small-Scale Targeted Screening
Sharlow, E. R. (2016) · Assay and Drug Development Technologies · doi:10.1089/adt.2016.734 · Cited by 2
Toward Modeling the Human Nervous System in a Dish: Recent Progress and Outstanding Challenges
Vagaská, B., & Ferretti, P. (2016) · Regenerative Medicine · doi:10.2217/rme-2016-0106 · Cited by 2
A Mouse Model of Alzheimer’s Disease with Transplanted Stem-Cell-Derived Human Neurons
Zhu, Y., & Zhang, H. (2017) · Neuroscience Bulletin · doi:10.1007/s12264-017-0180-8 · Cited by 2
Advances in Retinal Imaging: Retinal Amyloid Imaging
Koronyo‐Hamaoui, M., Doustar, J., Oviatt, M., Black, K. L., & Koronyo, Y. (2020) · Springer eBooks · doi:10.1007/978-3-030-26269-3_6 · Cited by 4
Three-Dimensional Models for Studying Neurodegenerative and Neurodevelopmental Diseases
Tsaridou, S., Skamnelou, M., Iliadou, M., Lokka, G., Parlapani, E., Mougkogianni, M., Danalatos, R.-I., Kanellou, A., Chlorogiannis, D., Kyrousi, C., et al. (2020) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-32633-3_6 · Cited by 3
3D Alzheimer's disease in a dish: Implications for drug discovery
Quinti, L., Park, J., Brand, E., Tanzi, R. E., & Kim, D. Y. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-816475-4.00014-8 · Cited by 2
Self-citationJia, X., Toda, K., He, L., Miao, D., Yamada, S., Yu, L., & Kodama, K. (2022) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/clinem/dgac256 · Cited by 2
Souza, R. T. V., Santana, P. H. G., Parreira, R. C., de Oliveira, L. F., Raminelli, A. O., Marques, B. L., & Filho, D. M. (2023) · Medicina (Ribeirão Preto) · doi:10.11606/issn.2176-7262.rmrp.2023.203797 · Cited by 2
Calcium Signaling during Cortical Apical Dendrite Initiation: A Role for Cajal-Retzius Neurons
Enck, J. R., & Olson, E. C. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms241612965 · Cited by 2
Network Bursts in 3D Neuron Clusters Cultured on Microcontact-Printed Substrates
Liang, Q., Chen, Z., Xie, C., Huang, Q., & Sun, T. (2023) · Micromachines · doi:10.3390/mi14091703 · Cited by 2
Novel Approaches to Studying SLC13A5 Disease
Beltrán, A. S. (2024) · Metabolites · doi:10.3390/metabo14020084 · Cited by 3
Maximizing the utility of brain organoid models and overcoming their perceived limitations
Wenzel, T. J., & Mousseau, D. D. (2024) · Elsevier eBooks · doi:10.1016/b978-0-323-95730-4.00009-3 · Cited by 2
McMurtrey, R. J. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-812636-3.00002-x · Cited by 2
Cuní‐López, C., Quek, H., Oikari, L. E., Stewart, R., Nguyen, T. H., Sun, Y., Guo, C. C., Lupton, M. K., & White, A. R. (2021) · Research Square · doi:10.21203/rs.3.rs-571254/v2 · Cited by 2
Effects of Aerobic Exercise on Brain Age and Health in Older Adults: A Single-Arm Clinical TrialPreprint
Ouyang, A., Zhang, C. M., Adra, N., Tesh, R. A., Sun, H., Lei, D., Jing, J., Fan, P., Paixão, L. G. P., Ganglberger, W., et al. (2022) · medRxiv · doi:10.1101/2022.06.13.22276337 · Cited by 2
Self-citationMicroengineered 2D and 3D modular neuronal networks represent structure-function relationshipPreprint
Habibey, R., Striebel, J., Latiftikhereshki, R., Schmieder, F., & Latifi, S. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.04.07.535751 · Cited by 2
Ojeda, A. V., Awabdeh, D., Brewster, B., Rockne, R. C., O’Meally, D., Yin, H. H., Carlesso, N., Brown, C. E., Gutova, M., & Barish, M. E. (2025) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2025.02.12.637976 · Cited by 2
Potential Regulatory Role of miR-21 on Alzheimer’s Disease by Targeting GSK-3β Signaling
Ding, H. (2023) · Galen Medical Journal · doi:10.31661/gmj.v12i.3027 · Cited by 1
Jawaid, T. (2024) · International Journal of Pharmacology · doi:10.3923/ijp.2024.22.33 · Cited by 1
Naghshbandieh, A. (2024) · American Journal of Stem Cells · doi:10.62347/bptg6174 · Cited by 1
Aksu‐Menges, E., Kumtepe, E. T., Akpınar, G., & Balcı-Hayta, B. (2025) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.70370 · Cited by 1
Tzekaki, E., Bekiari, C., Pantazaki, Α. A., Tsantarliotou, M. P., Tsolaki, M., & Lavrentiadou, S. N. (2025) · Journal of Neuroscience Methods · doi:10.1016/j.jneumeth.2025.110404 · Cited by 1
Seo, M. K., Jeong, S., Cheon, W. S., Lee, D. Y., Lee, S., Lee, G.-H., Kang, D.-K., Seog, D., Kim, S.-H., Lee, J. G., et al. (2025) · Clinical Psychopharmacology and Neuroscience · doi:10.9758/cpn.25.1269 · Cited by 1
Self-citationAI for Neurodegenerative Experimental Models: Advancements and Applications
Alkubaisy, M. M., Khazaal, W. M., Shakir, K. H., Muhammad, S. R., Habelalmateen, M. I., Ayad, H., & Saadoun, O. N. (2024) · International Conference on IoT, Communication and Automation Technology (ICICAT) · doi:10.1109/icicat62666.2024.10923433 · Cited by 1
Chen, Z., Huang, C., Liu, H., Wang, Z., Xu, L., Huang, S., Qu, M., & Xu, Z. (2025) · Colloids and Surfaces A Physicochemical and Engineering Aspects · doi:10.1016/j.colsurfa.2025.136916 · Cited by 1
Volloch, V., & Rits‐Volloch, S. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26094252 · Cited by 3
Sepehrimanesh, M., Wu, X., & Ding, B. (2025) · Neural Regeneration Research · doi:10.4103/nrr.nrr-d-24-00435 · Cited by 1
Edwards, S., Guan, Z., Ganash, M., Cuvellier, H., Reynolds, J., Bartus, A., Kim, Y. J., Timko, B. P., & Jeong, K. J. (2025) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.4c02247 · Cited by 1
Self-citationScalable human neuronal models of tauopathy producing endogenous seed-competent 4R tau
Tsefou, E., Bez, S., Birkle, T. J. Y., Foiani, M. S., Watamura, N., Bourdenx, M., Gavriouchkina, D., Turkes, E., Crawford, S., Coneys, R., et al. (2026) · Science Advances · doi:10.1126/sciadv.aeg1445 · Cited by 1
Zhao, J., Wang, J., & Guo, X. (2025) · Neural Regeneration Research · doi:10.4103/nrr.nrr-d-25-00924 · Cited by 1
Dual Targeting of Tau Kinases and Autophagy by Abemaciclib Independent of CDK4/6 Inhibition
Han, J., Jeong, J., Lee, D., Jung, Y., Jin, Y., Jung, E. S., Kim, H. J., Kim, W. Y., Lee, C., & Mook‐Jung, I. (2026) · Advanced Science · doi:10.1002/advs.202513135 · Cited by 1
Motamed, Z., Novack, G. V., Heutschi, D., Gaiser, C., Garcia, C., Carmo, S. D., Cuello, A. C., Morelli, L., & Suter‐Dick, L. (2026) · Neurobiology of Aging · doi:10.1016/j.neurobiolaging.2026.01.006 · Cited by 1
Maaser‐Hecker, A., Zellmer, J. C., Kim, M. M., Bakiasi, G., Maiti, A. K., Curtat, M. P. C., Choi, S. H., Prokopenko, D., Tanzi, R. E., & Bhattacharyya, R. (2026) · Science Advances · doi:10.1126/sciadv.adx2127 · Cited by 2
Self-citationSun, F., Liu, K., Xi, E., Zhao, Y. W., & Gao, N. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27062871 · Cited by 1
Hebisch, M., Kamin, V., Cenini, G., Piazzesi, A., Bertan, F., Weykopf, B., Schlee, J., Kaniyappan, S., Washicosky, K. J., Kim, D. Y., et al. (2026) · Cell Reports Methods · doi:10.1016/j.crmeth.2026.101365 · Cited by 1
Self-citationLuo, J., Jiang, J., Zhang, T., Zhang, X., Huang, Y., Zhang, X., Wang, Y., & Yang, Y. (2026) · Frontiers in Endocrinology · doi:10.3389/fendo.2026.1736356 · Cited by 1
Three-dimensional direct neuronal reprogramming for modeling Alzheimer’s disease neuropathology
Sun, Z., Lee, Y., Walker, C. K., Karch, C. M. M., & Yoo, A. S. J. (2026) · Nature Protocols · doi:10.1038/s41596-026-01388-0 · Cited by 1
Li, Q., Zhang, E., Liu, B., Deng, Y., Wang, Y., Fan, K., & Wang, Z. (2026) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.6c00234 · Cited by 1
Combating ageing beyond the cell: Emerging roles of extracellular proteostasis
Venkatesh, S. R., Gallotta, I., Olabiyi, B. F., Choi, Y. H., Inglesfield, S., Kumita, J. R., Ewald, C. Y., & David, D. C. (2026) · FEBS Journal · doi:10.1111/febs.70647 · Cited by 2
Khan, M. S., Zafar, I., & Bopassa, J. C. (2026) · International Journal of Molecular Sciences · doi:10.3390/ijms27146329 · Cited by 1
Metadata
- Authors
- Choi, S. H., Kim, Y. H., Hebisch, M., Sliwinski, C., Lee, S., D’Avanzo, C., Chen, H., Hooli, B., Asselin, C., Muffat, J., Klee, J. B., Zhang, C., Wainger, B. J., Peitz, M., Kovacs, D. M., Woolf, C. J., Wagner, S. L., Tanzi, R. E., & Kim, D. Y.
- Affiliations
- Harvard University (US)
- University of Bonn (DE)
- Boston Children's Hospital (US)
- Whitehead Institute for Biomedical Research (US)
- University of California San Diego (US)
- Corresponding author
- Tanzi, Rudolph E.
- Kim, Doo Yeon
- ORCID
- Venue
- Nature
- Published
- 2014-10-12
- Volume
- 515
- Issue
- 7526
- Pages
- 274-278
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC4366007
- OpenAlex
- W2126231259
- Cited by
- 1,195
- References
- 30
- Citations by year
- 2026: 67 · 2025: 75 · 2024: 95 · 2023: 88 · 2022: 112 · 2021: 111 · 2020: 141 · 2019: 109 · 2018: 109 · 2017: 87 · 2016: 97 · 2015: 87 · 2014: 14
- FWCI
- 63.56
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature (publishedVersion, public-domain)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Digital Access to Scholarship at Harvard (DASH) (Harvard University) (submittedVersion, cc-by)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- Massachusetts General Hospital
- Cure Alzheimer's Fund
- University of Edinburgh
- National Institute on Aging (5P01 AG15379, P01AG015379, R21AG031483, RF1AG048080, P50AG005134, R01AG014713, P01AG004953, P30AG062421)
- National Institute of Mental Health (R37MH060009)
- National Institute of Diabetes and Digestive and Kidney Diseases (P30DK057521, P30DK043351)
- National Institute of Neurological Disorders and Stroke (P30NS045776, R01NS045860)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (P30HD018655)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
- Keywords
- Alzheimer's disease, familial Alzheimer's disease, amyloid plaques, tauopathy, neurofibrillary tangles, amyloid hypothesis, presenilin 1 mutations, human neural stem cells, 3D cell culture, human neurons, beta-secretase inhibition, gamma-secretase inhibition, tau phosphorylation, drug screening model
- MeSH terms
- Alzheimer Disease, Cell Differentiation, Drug Evaluation, Preclinical, Extracellular Space, Humans, Microtubule-Associated Proteins, Models, Biological, Phosphorylation, Reproducibility of Results, Amyloid beta-Peptides, Neurites, tau Proteins, Cell Culture Techniques, Glycogen Synthase Kinase 3, Presenilin-1, Neural Stem Cells, Protein Aggregation, Pathological
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
