The DOI matches its Crossref record; 82 of the 82 references it cites were found in a registry or an index.
Visualizing transient Watson–Crick-like mispairs in DNA and RNA duplexes
Open accessAbstract
Rare tautomeric and anionic nucleobases are believed to have fundamental biological roles, but their prevalence and functional importance has remained elusive because they exist transiently, in low abundance, and involve subtle movements of protons that are difficult to visualize. Using NMR relaxation dispersion, we show here that wobble dG•dT and rG•rU mispairs in DNA and RNA duplexes exist in dynamic equilibrium with short-lived, low-populated Watson-Crick-like mispairs that are stabilized by rare enolic or anionic bases. These mispairs can evade Watson-Crick fidelity checkpoints and form with probabilities (10(-3) to 10(-5)) that strongly imply a universal role in replication and translation errors. Our results indicate that rare tautomeric and anionic bases are widespread in nucleic acids, expanding their structural and functional complexity beyond that attainable with canonical bases.
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“More recently, tautomerisation in DNA has been experimentally observed using NMR relaxation dispersion.Here, hairpin DNA duplex structures were observed to interchange between a wobble G-T and a Watson-Crick-like structure via tautomerisation (neutral) or ionisation (charged) 9,51 .”
From An open quantum systems approach to proton tunnelling in DNA (2022) · cites this paper as [51]
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Cites
Zhao, Y., & Truhlar, D. G. (2007) · Theoretical Chemistry Accounts · doi:10.1007/s00214-007-0310-x · Cited by 30,666
NMRPipe: A multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G. W., Zhu, G., Pfeifer, J., & Bax, A. (1995) · Journal of Biomolecular NMR · doi:10.1007/bf00197809 · Cited by 15,961
Protein NMR Spectroscopy: Principles and Practice
Cavanagh, J. (1995) · Cited by 1,642
Reaction Rates by Nuclear Magnetic Resonance
McConnell, H. M. (1958) · The Journal of Chemical Physics · doi:10.1063/1.1744152 · Cited by 1,624
THE STRUCTURE OF DNAPreprint
Watson, J. D., & Crick, F. H. C. (1953) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.1953.018.01.020 · Cited by 1,203
Kunkel, T. A., & Bebenek, K. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.497 · Cited by 1,003
Complementary base pairing and the origin of substitution mutations
Topal, M. D., & Fresco, J. R. (1976) · Nature · doi:10.1038/263285a0 · Cited by 843
Kunkel, T. A. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.r400006200 · Cited by 754
Selection of tRNA by the Ribosome Requires a Transition from an Open to a Closed Form
Ogle, J. M., Murphy, F. V., Tarry, M. J., & Ramakrishnan, V. (2002) · Cell · doi:10.1016/s0092-8674(02)01086-3 · Cited by 663
STRUCTURAL INSIGHTS INTO TRANSLATIONAL FIDELITY
Ogle, J. M., & Ramakrishnan, V. (2005) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.74.061903.155440 · Cited by 596
Research works printed but not linked: 3
- Brovarets’, O. O. Effect of a modification of uracil on the tautomerization barrier of the wobble Gua•5XUra base pair into the Gua*•5XUra base pair with the Watson-Crick geometry: quantum-chemical study. Rep. Natl. Acad. Sci. Ukraine 4, 154–158 (2013)
- Kochina, O. S., Zhurakivsky, R. O. & Hovorun, D. M. Effect of the nucleotide bases tautomerization on the conformational properties of the nucleosides: quantum-mechanical investigation by the DFT method. Rep. Natl. Acad. Sci. Ukraine 1, 181–186 (2008)
- Brovarets’, O. O. & Hovorun, D. M. Physicochemical mechanism of the wobble DNA base pairs Gua•Thy and Ade•Cyt transition into the mismatched base pairs Gua*•Thy and Ade•Cyt* formed by the mutagenic tautomers. Ukr. Bioorg. Acta 8, 12–18 (2009)
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Stem cell divisions, somatic mutations, cancer etiology, and cancer prevention
Tomasetti, C., Li, L., & Vogelstein, B. (2017) · Science · doi:10.1126/science.aaf9011 · Cited by 1,143
RNA Structural Dynamics As Captured by Molecular Simulations: A Comprehensive Overview
Šponer, J., Bussi, G., Krepl, M., Banáš, P., Bottaro, S., Cunha, R. A., Gil-Ley, A., Pinamonti, G., Poblete, S., Jurečka, P., et al. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00427 · Cited by 606
The roles of structural dynamics in the cellular functions of RNAs
Ganser, L. R., Kelly, M. L., Herschlag, D., & Al‐Hashimi, H. M. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0136-0 · Cited by 563
Self-citationDSSR: an integrated software tool for dissecting the spatial structure of RNA
Lu, X.-J., Bussemaker, H., & Olson, W. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv716 · Cited by 349
Fundamental studies of functional nucleic acids: aptamers, riboswitches, ribozymes and DNAzymes
Micura, R., & Höbartner, C. (2020) · Chemical Society Reviews · doi:10.1039/d0cs00617c · Cited by 265
Metadata
- Authors
- Kimsey, I. J., Petzold, K., Sathyamoorthy, B., Stein, Z. W., & Al-Hashimi, H. M.
- Affiliations
- Duke University (US)
- Karolinska Institutet (SE)
- University of Michigan (US)
- Venue
- Nature
- Published
- 2015-03-11
- Volume
- 519
- Issue
- 7543
- Pages
- 315-320
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- PMC
- PMC4547696
- OpenAlex
- W2066558081
- Cited by
- 261
- References
- 82
- Citations by year
- 2026: 14 · 2025: 20 · 2024: 14 · 2023: 17 · 2022: 23 · 2021: 33 · 2020: 20 · 2019: 20 · 2018: 39 · 2017: 29 · 2016: 20 · 2015: 12
- FWCI
- 14.48
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- National Institute of General Medical Sciences (R01GM089846)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
- Keywords
- tautomerism, enol tautomer, double-stranded DNA, RNA duplex, NMR relaxation dispersion, replication fidelity, translation errors
- MeSH terms
- Base Sequence, DNA, Hydrogen Bonding, Hydrogen-Ion Concentration, Mutation, Magnetic Resonance Spectroscopy, Nucleic Acid Heteroduplexes, Probability, RNA, DNA Fingerprinting, Base Pairing
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