The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
Reversible methylation of m6Am in the 5′ cap controls mRNA stability
Open accessAbstract
Internal bases in mRNA can be subjected to modifications that influence the fate of mRNA in cells. One of the most prevalent modified bases is found at the 5′ end of mRNA, at the first encoded nucleotide adjacent to the 7-methylguanosine cap. Here we show that this nucleotide, N6,2′-O-dimethyladenosine (m6Am), is a reversible modification that influences cellular mRNA fate. Using a transcriptome-wide map of m6Am we find that m6Am-initiated transcripts are markedly more stable than mRNAs that begin with other nucleotides. We show that the enhanced stability of m6Am-initiated transcripts is due to resistance to the mRNA-decapping enzyme DCP2. Moreover, we find that m6Am is selectively demethylated by fat mass and obesity-associated protein (FTO). FTO preferentially demethylates m6Am rather than N6-methyladenosine (m6A), and reduces the stability of m6Am mRNAs. Together, these findings show that the methylation status of m6Am in the 5′ cap is a dynamic and reversible epitranscriptomic modification that determines mRNA stability.
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Cites
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Self-citationN6-Methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO
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Cited by
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R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling
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The interplay between m6A RNA methylation and noncoding RNA in cancer
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Metadata
- Authors
- Mauer, J., Luo, X., Blanjoie, A., Jiao, X., Grozhik, A. V., Patil, D. P., Linder, B., Pickering, B. F., Vasseur, J.-J., Chen, Q., Gross, S. S., Elemento, O., Debart, F., Kiledjian, M., & Jaffrey, S. R.
- Venue
- Nature
- Published
- 2016-12-21
- Volume
- 541
- Issue
- 7637
- Pages
- 371-375
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2562211106
- Cited by
- 1,062
- References
- 13
- FWCI
- 39.73
- Type
- journal article
- Language
- en
- Open access
- green
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- Mauer, J., Luo, X., Blanjoie, A., Jiao, X., Grozhik, A. V., Patil, D. P., Linder, B., Pickering, B. F., Vasseur, J.-J., Chen, Q., Gross, S. S., Elemento, O., Debart, F., Kiledjian, M., & Jaffrey, S. R. (2017). Reversible methylation of m6Am in the 5′ cap controls mRNA stability. Nature. https://doi.org/10.1038/nature21022
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