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Cited by 385

The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.

Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture

Open access
Forrester, Michael T, Thompson, J Will, Foster, Matthew W, Nogueira, Leonardo, Moseley, M Arthur, Stamler, Jonathan S·2009·Nature Biotechnology

Abstract

We have modified the biotin switch assay for protein S-nitrosothiols (SNOs), using resin-assisted capture (SNO-RAC). Compared with existing methodologies, SNO-RAC requires fewer steps, detects high-mass S-nitrosylated proteins more efficiently, and facilitates identification and quantification of S-nitrosylated sites by mass spectrometry. When combined with iTRAQ labeling, SNO-RAC revealed that intracellular proteins may undergo rapid denitrosylation on a global scale. This methodology is readily adapted to analyzing diverse cysteine-based protein modifications, including S-acylation.

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Metadata

Authors
Forrester, M. T., Thompson, J. W., Foster, M. W., Nogueira, L., Moseley, M. A., & Stamler, J. S.
Venue
Nature Biotechnology
Published
2009-05-31
Volume
27
Issue
6
Pages
557-559
ISSN
  • 0733-222X (ISSN-L)
  • 1087-0156
  • 1546-1696
OpenAlex
W1991454533
Cited by
385
References
14
FWCI
12.24
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture. https://app.cochat.ai/papers/10.1038/nbt.1545
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Forrester, M. T., Thompson, J. W., Foster, M. W., Nogueira, L., Moseley, M. A., & Stamler, J. S. (2009). Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture. Nature Biotechnology. https://doi.org/10.1038/nbt.1545

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