The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
Inhibition of natural antisense transcripts in vivo results in gene-specific transcriptional upregulation
Open accessAbstract
The ability to specifically upregulate genes in vivo holds great therapeutic promise. Here we show that inhibition or degradation of natural antisense transcripts (NATs) by single-stranded oligonucleotides or siRNAs can transiently and reversibly upregulate locus-specific gene expression. Brain-derived neurotrophic factor (BDNF) is normally repressed by a conserved noncoding antisense RNA transcript, BDNF-AS. Inhibition of this transcript upregulates BDNF mRNA by two- to sevenfold, alters chromatin marks at the BDNF locus, leads to increased protein levels and induces neuronal outgrowth and differentiation both in vitro and in vivo. We also show that inhibition of NATs leads to increases in glial-derived neurotrophic factor (GDNF) and ephrin receptor B2 (EPHB2) mRNA. Our data suggest that pharmacological approaches targeting NATs can confer locus-specific gene upregulation effects.
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Cites
Functional Demarcation of Active and Silent Chromatin Domains in Human HOX Loci by Noncoding RNAs
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Self-citationPolycomb Proteins Targeted by a Short Repeat RNA to the Mouse X Chromosome
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Self-citationEndogenous siRNAs from naturally formed dsRNAs regulate transcripts in mouse oocytes
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The complex transcriptional landscape of the anucleate human platelet
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Metadata
- Authors
- Modarresi, F., Faghihi, M. A., Lopez-Toledano, M. A., Fatemi, R. P., Magistri, M., Brothers, S. P., van der Brug, M. P., & Wahlestedt, C.
- Venue
- Nature Biotechnology
- Published
- 2012-03-25
- Volume
- 30
- Issue
- 5
- Pages
- 453-459
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2154942988
- Cited by
- 668
- References
- 51
- FWCI
- 15.84
- Type
- journal article
- Language
- en
- Open access
- green
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- Modarresi, F., Faghihi, M. A., Lopez-Toledano, M. A., Fatemi, R. P., Magistri, M., Brothers, S. P., van der Brug, M. P., & Wahlestedt, C. (2012). Inhibition of natural antisense transcripts in vivo results in gene-specific transcriptional upregulation. Nature Biotechnology. https://doi.org/10.1038/nbt.2158
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