The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Compartmentalization of metabolic pathways in yeast mitochondria improves the production of branched-chain alcohols
Open accessAbstract
Efforts to improve the production of a compound of interest in Saccharomyces cerevisiae have mainly involved engineering or overexpression of cytoplasmic enzymes. We show that targeting metabolic pathways to mitochondria can increase production compared with overexpression of the enzymes involved in the same pathways in the cytoplasm. Compartmentalization of the Ehrlich pathway into mitochondria increased isobutanol production by 260%, whereas overexpression of the same pathway in the cytoplasm only improved yields by 10%, compared with a strain overproducing enzymes involved in only the first three steps of the biosynthetic pathway. Subcellular fractionation of engineered strains revealed that targeting the enzymes of the Ehrlich pathway to the mitochondria achieves greater local enzyme concentrations. Other benefits of compartmentalization may include increased availability of intermediates, removing the need to transport intermediates out of the mitochondrion and reducing the loss of intermediates to competing pathways.
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Cites
Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
Atsumi, S., Hanai, T., & Liao, J. C. (2008) · Nature · doi:10.1038/nature06450 · Cited by 1,945
Multifunctional yeast high-copy-number shuttle vectors
Christianson, T. W., Sikorski, R. S., Dante, M., Shero, J. H., & Hieter, P. (1992) · Gene · doi:10.1016/0378-1119(92)90454-w · Cited by 1,658
Hazelwood, L. A., Daran, J.-M., van Maris, A. J. A., Pronk, J. T., & Dickinson, J. R. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.02625-07 · Cited by 1,547
Attardi, G., & Schatz, G. (1988) · Annual Review of Cell Biology · doi:10.1146/annurev.cb.04.110188.001445 · Cited by 1,387
Subcellular localization of the yeast proteome
Kumar, A., Agarwal, S., Heyman, J. A., Matson, S., Heidtman, M., Piccirillo, S., Umansky, L., Drawid, A., Jansen, R., Liu, Y., et al. (2002) · Genes & Development · doi:10.1101/gad.970902 · Cited by 739
Engineered reversal of the β-oxidation cycle for the synthesis of fuels and chemicals
Dellomonaco, C., Clomburg, J. M., Miller, E. N., & Gonzalez, R. (2011) · Nature · doi:10.1038/nature10333 · Cited by 596
Hong, K.-K., & Nielsen, J. (2012) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-0945-1 · Cited by 451
Redirection of cytosolic or plastidic isoprenoid precursors elevates terpene production in plants
Wu, S., Schalk, M., Clark, A., Miles, R. B., Coates, R., & Chappell, J. (2006) · Nature Biotechnology · doi:10.1038/nbt1251 · Cited by 408
Orij, R., Postmus, J., Ter Beek, A., Brul, S., & Smits, G. J. (2009) · Microbiology · doi:10.1099/mic.0.022038-0 · Cited by 364
Iron–sulfur-protein biogenesis in eukaryotes
Lill, R., & Mühlenhoff, U. (2005) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2005.01.006 · Cited by 333
Cited by
Engineering Cellular Metabolism
Nielsen, J., & Keasling, J. D. (2016) · Cell · doi:10.1016/j.cell.2016.02.004 · Cited by 1,378
Zhou, K., Qiao, K., Edgar, S., & Stephanopoulos, G. (2015) · Nature Biotechnology · doi:10.1038/nbt.3095 · Cited by 710
Self-citationPires, E. J., Teixeira, J. A., Brányik, T., & Vicente, A. A. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-013-5470-0 · Cited by 613
Poornima, S., Manikandan, S., Prakash, R., Deena, S. R., Subbaiya, R., Karmegam, N., Kim, W., & Govarthanan, M. (2024) · Fuel · doi:10.1016/j.fuel.2024.132204 · Cited by 113
Liu, X., Xie, H., Roussou, S., & Lindblad, P. (2022) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2021.07.014 · Cited by 62
Metadata
- Authors
- Avalos, J. L., Fink, G. R., & Stephanopoulos, G.
- Venue
- Nature Biotechnology
- Published
- 2013-02-17
- Volume
- 31
- Issue
- 4
- Pages
- 335-341
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W1990060260
- Cited by
- 517
- References
- 39
- FWCI
- 17.75
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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