The DOI matches its Crossref record; 39 of the 39 references it cites were found in a registry or an index.
Distributing a metabolic pathway among a microbial consortium enhances production of natural products
Open accessAbstract
Metabolic engineering of microorganisms such as Escherichia coli and Saccharomyces cerevisiae to produce high-value natural metabolites is often done through functional reconstitution of long metabolic pathways. Problems arise when parts of pathways require specialized environments or compartments for optimal function. Here we solve this problem through co-culture of engineered organisms, each of which contains the part of the pathway that it is best suited to hosting. In one example, we divided the synthetic pathway for the acetylated diol paclitaxel precursor into two modules, expressed in either S. cerevisiae or E. coli, neither of which can produce the paclitaxel precursor on their own. Stable co-culture in the same bioreactor was achieved by designing a mutualistic relationship between the two species in which a metabolic intermediate produced by E. coli was used and functionalized by yeast. This synthetic consortium produced 33 mg/L oxygenated taxanes, including a monoacetylated dioxygenated taxane. The same method was also used to produce tanshinone precursors and functionalized sesquiterpenes.
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Cites
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Self-citationMicrobial production of short-chain alkanes
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Cited by
Liew, F., Martin, M. E., Tappel, R. C., Heijstra, B. D., Mihalcea, C., & Köpke, M. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00694 · Cited by 485
Designing microbial consortia with defined social interactions
Kong, W., Meldgin, D. R., Collins, J. J., & Lu, T. (2018) · Nature Chemical Biology · doi:10.1038/s41589-018-0091-7 · Cited by 327
Construction of microbial consortia for microbial degradation of complex compounds
Cao, Z., Yan, W., Ding, M., & Yuan, Y. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.1051233 · Cited by 274
Biotechnological basis of microbial consortia for the removal of pesticides from the environment
Bhatt, P., Bhatt, K., Sharma, A., Zhang, W., Mishra, S., & Chen, S. (2021) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2020.1853032 · Cited by 248
Engineered microbial consortia: strategies and applications
Duncker, K. E., Holmes, Z. A., & You, L. (2021) · Microbial Cell Factories · doi:10.1186/s12934-021-01699-9 · Cited by 196
Metadata
- Authors
- Zhou, K., Qiao, K., Edgar, S., & Stephanopoulos, G.
- Venue
- Nature Biotechnology
- Published
- 2015-01-05
- Volume
- 33
- Issue
- 4
- Pages
- 377-383
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W1998240064
- Cited by
- 710
- References
- 39
- FWCI
- 19.81
- Type
- journal article
- Language
- en
- Open access
- green
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