Engineered CHO cells for production of diverse, homogeneous glycoproteins

Yang, Zhang, Wang, Shengjun, Halim, Adnan, Schulz, Morten Alder, Frodin, Morten, Rahman, Shamim H, Vester-Christensen, Malene B, Behrens, Carsten, Kristensen, Claus, Vakhrushev, Sergey Y, Bennett, Eric Paul, Wandall, Hans H, Clausen, Henrik·2015·Nature Biotechnology
DOI 10.1038/nbt.3280

Cited by (254)

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  9. Linear and neural network models for predicting N-glycosylation in Chinese Hamster Ovary cells based on B4GALT levels
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  10. Click-chemistry enabled directed evolution of glycosynthases for bespoke glycans synthesis
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  11. Identification of novel miRNA targets in CHO cell lines and characterization of their impact on protein N-glycosylation
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  12. Decoding Protein Glycosylation for Better Vaccine and Antibody Development
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  13. Metabolic and Process Engineering to Control Glycan Structures for Biopharmaceuticals Produced in Cultured Mammalian Cells
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  14. Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
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  15. CRISPR Toolbox for Mammalian Cell Engineering
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  16. Cell-based production of Fc-GlcNAc and Fc-alpha-2,6 sialyl glycan enriched antibody with improved effector functions through glycosylation pathway engineering
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  17. Membrane glycoproteins get another go: the GlycoSwitch
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  18. CRISPR-based precise methylation of specific FUT8 promoter regions allows isolation of CHO cells with a fine-tuned glycoprofile
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  19. Precision glycoform engineering: Combining plant and in vitro systems for tailored biopharmaceutical production
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  20. Impact of mutations on the plant-based production of recombinant SARS-CoV-2 RBDs
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  22. Antibody-dependent cellular cytotoxicity-null effector developed using mammalian and plant GlycoDelete platform
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  23. Expression Systems for Recombinant Biopharmaceutical Production by Mammalian Cells in Culture
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  24. Enhancing Product and Bioprocess Attributes Using Genome‐Scale Models of CHO Metabolism
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  25. N ‐glycosylation of Proteins
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  26. Galectin-1 upregulates IDO1 and PD-L1 and induces a tolerogenic tumor-associated macrophage phenotype
    Asha M. Rudjord-Levann, Zilu Ye, Lise Hafkenscheid, Sabrina Horn, Renske Wiegertjes, Mathias Ingemann Nielsen, Caroline Benedicte Kjærulff Mathiesen, Jesse Stoop, Sean R. Stowell, Per thor Straten et al. · 2022 · Research Square
  27. Targeting Carbohydrates in Cancer – Analytical and Biotechnological Tools
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  28. Biantennary N-glycans As Receptors for MARTX Toxins in Vibrio Pathogenesis
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  29. Application of liquid chromatography-mass spectrometry-based protein and proteomic analytical approaches to chinese hamster ovary cell based industrial biopharmaceutical production
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  30. Metabolite Profiling of Mammalian Cells
    Claire E. Gaffney, Alan James Dickson, Mark Elvin · 2019
  31. Towards the cell-free expression of glycoproteins
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  32. Characterization of an efficient extracellular cyanophycinase and its encoding cphE gene from Streptomyces pratensis strain YSM
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  33. Glycoengineering of Chinese Hamster Ovary cells for improving biotherapeutics’ efficacies.
    Cheng‐Yu Chung · 2016
  34. Protein Engineering with A Glycosylation Circuit Enables Improved Enzyme Characteristics
    Eray Ulaş Bozkurt, İrem Niran Çağıl, Ebru Şahin Kehribar, Musa Efe Işılak, Urartu Özgür Şafak Şeker · 2021 · bioRxiv (Cold Spring Harbor Laboratory)
  35. Glycosylation
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  36. Monoclonal Antibody Glycoengineering for Biopharmaceutical Quality Assurance
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  37. Glycoproteins
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  38. Preparing glycomics data for robust statistical analysis with GlyCompareCT
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  39. Native Mass Spectrometry of Glycoproteins
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  40. Glycoengineering of the hepatitis C virus E2 glycoprotein leads to improved biochemical properties and enhanced immunogenicity
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  41. Glycosylated IgG antibodies contribute to the recovery of haemorrhagic fever with renal syndrome patients
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  42. A Multi Clone Kinetic Model for characterizing Chinese hamster ovary cell line variability
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  43. Glycosylated IgG antibodies accelerated the recovery of haemorrhagic fever with renal syndrome patients
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  44. Glycosylated IgG antibodies contribute to the recovery of haemorrhagic fever with renal syndrome patients
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  45. Genetic Factors Affecting the Quality of Recombinant Therapeutic Proteins in CHO Cells
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  46. Asymmetrical Glyco-Engineering of Monoclonal Antibodies Provides Insights into α-Gal Immunogenicity
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  48. The Role of Glycan Structures in Modulating GM-CSF Bioactivity: Insights from Glycoengineering
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  49. Delineating the Transcriptional and Phenotypic Impact from Biotherapeutic Glycoengineering
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  50. Mapping ST3GAL transferase specificities in the glycosylation landscape of N/TERT-1 keratinocytes using Glycogenomics and mass spectrometry
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  52. Base editing-mediated exon-specific knockout of glutamine synthetase generates a high-productivity CHO cell line for antibody manufacturing
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  53. Improving recombinant protein productivity in CHO cells via multi-omics data integration
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  54. Plant-Based Systems for Producing Therapeutic Proteins: Current Status and Future Prospects
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