The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
High-throughput determination of the antigen specificities of T cell receptors in single cells
Open accessPublished version of a preprint
Abstract
We present tetramer-associated T-cell receptor sequencing (TetTCR-seq) to link T cell receptor (TCR) sequences to their cognate antigens in single cells at high throughput. Binding is determined using a library of DNA-barcoded antigen tetramers that is rapidly generated by in vitro transcription and translation. We applied TetTCR-seq to identify patterns in TCR cross-reactivity with cancer neoantigens and to rapidly isolate neoantigen-specific TCRs with no cross-reactivity to the wild-type antigen.
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“However, these capture sequences are not guide-specific, and thus, in principle, will enable multiplexed capture of additional features, such as antibodies22,23 and other oligo-tagged markers24.”
From Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing (2020) · cites this paper as [24]
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“Although AIR specificity can be experimentally determined using barcoded antigens177,178, several approaches attempt to infer it computationally (Fig. 5e).”
From Best practices for single-cell analysis across modalities (2023) · cites this paper as [178]
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“Cellular organization bridges the gap between tissue biology and pathology, which enables the discovery of new cellular functionalities and creates new computational challenges for which distinct analysis methods are required 190–192 .”
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Cossarizza, A., Chang, H., Radbruch, A., Acs, A., Adam, D., Adam‐Klages, S., Agace, W. W., Aghaeepour, N., Akdiş, M., Allez, M., et al. (2019) · European Journal of Immunology · doi:10.1002/eji.201970107 · Cited by 1,007
Dynamics and specificities of T cells in cancer immunotherapy
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)
Cossarizza, A., Chang, H., Radbruch, A., Abrignani, S., Addo, R., Akdis, M., Andrä, I., Andreata, F., Annunziato, F., Arranz, E., et al. (2021) · European Journal of Immunology · doi:10.1002/eji.202170126 · Cited by 531
Metadata
- Authors
- Zhang, S.-Q., Ma, K.-Y., Schonnesen, A. A., Zhang, M., He, C., Sun, E., Williams, C. M., Jia, W., & Jiang, N.
- Affiliations
- The University of Texas at Austin (US)
- Shanghai Jiao Tong University (CN)
- Corresponding author
- Jia, Weiping
- Jiang, Ning
- Venue
- Nature Biotechnology
- Published
- 2018-11-12
- Volume
- 36
- Issue
- 12
- Pages
- 1156-1159
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- PMC
- PMC6728224
- OpenAlex
- W2899327929
- Cited by
- 200
- References
- 21
- Citations by year
- 2026: 23 · 2025: 23 · 2024: 19 · 2023: 36 · 2022: 21 · 2021: 27 · 2020: 28 · 2019: 20 · 2018: 3
- FWCI
- 8.38
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- cc-by-nc-nd
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- University of Texas at Austin
- Chinese Academy of Sciences
- National Institutes of Health (1s10od020072-01)
- Institute of Biophysics, Chinese Academy of Sciences
- National Institute on Aging (R00AG040149)
- National Cancer Institute (R33CA225539)
- National Institute of Biomedical Imaging and Bioengineering (T32EB007507)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Oncology › Cancer Immunotherapy and Biomarkers
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Keywords
- T cell receptor sequencing, antigen specificity, T cell receptor cross-reactivity, neoantigens, neoantigen-specific T cells, in vitro transcription and translation
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