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Mixed Lactic Acid–Alcoholic Fermentation by Saccharomyes cerevisiae Expressing the Lactobacillus casei L(+)–LDH
Abstract
We describe the construction of a Saccharomyces cerevisiae strain expressing the gene encoding the L(+)-lactate dehydrogenase [L(+)-LDH)] from Lactobacillus casei. The recombinant strain is able to perform a mixed lactic acid-alcoholic fermentation. Yeast cells expressing the L(+)-LDH gene from the yeast alcohol dehydrogenase (ADH1) promoter on a multicopy plasmid simultaneously convert glucose to both ethanol and lactate, with up to 20% of the glucose transformed into L(+)-lactate. Such strains may be used in every field where both biological acidification and alcoholic fermentation are required.
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“Due to the inefficiency of the mitochondrial lacticodehydrogenases under fermentation conditions, natural Saccharomyces cerevisiae strains produce only traces of lactic acid during alcoholic fermentation (Dequin and Barre 1994).”
From Yeast and bacterial modulation of wine aroma and flavour (2005) · cites this paper as Dequin and Barre 1994
In the citing paper
“Researchers have successfully employed genetic engineering to construct Saccharomyces cerevisiae strains capable of modulating the concentrations of lactic acid and malic acid.”
From Yeast and bacterial modulation of wine aroma and flavour (2005) · cites this paper as Dequin and Barre 1994
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Metadata
- Authors
- Dequin, S., & Barre, P.
- Affiliations
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- ORCID
- Dequin, Sylvie
- Venue
- Nature Biotechnology
- Published
- 1994-02
- Volume
- 12
- Issue
- 2
- Pages
- 173-177
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W1972332967
- Cited by
- 145
- References
- 32
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 2 · 2022: 5 · 2021: 4 · 2020: 3 · 2019: 3 · 2018: 5 · 2017: 5 · 2016: 4 · 2015: 6 · 2014: 10 · 2013: 6 · 2012: 6
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Keywords
- Saccharomyces cerevisiae, L-lactate dehydrogenase, Lactobacillus casei, recombinant yeast, ethanol production, lactic acid production, ADH1 promoter, multicopy plasmid, biological acidification, heterologous expression
- MeSH terms
- Alcohol Dehydrogenase, Ethanol, Base Sequence, Cloning, Molecular, Fermentation, Genetic Vectors, Glucose, L-Lactate Dehydrogenase, Lactates, Lacticaseibacillus casei, Molecular Sequence Data, Oligodeoxyribonucleotides, Promoter Regions, Genetic, Recombinant Proteins, Saccharomyces cerevisiae, Restriction Mapping, Polymerase Chain Reaction, Mutagenesis, Site-Directed, Lactic Acid
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