Chinese hamster ovary cells can produce galactose-α-1,3-galactose antigens on proteins
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CHO cells produce glycans that do not occur in human cells, namely Neu5Gc and α-gal, at very low levels (<2% and <0.2% respectively).
“Moreover, CHO cells produce glycans that do not occur in human cells, namely N-glycolylneuraminic acid (Neu5Gc) and galactose-α1,3-galactose (α-gal), even though these occurring at very low levels (e.g < 2% and < 0.2% respectively) (Bosques et al., 2010;Ghaderi et al., 2010;Dietmair et al., 2012b;Ghaderi et al., 2012).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Bosques et al., 2010
In “To the Editor” (page unavailable), the paper shows that CHO-produced proteins can contain α-Gal and reports an α-Gal–containing glycan in abatacept at approximately 0.2% of total N-linked glycans. That is a product-specific estimate, not a general level below 0.2%. Our copy does not address Neu5Gc or its claimed level below 2%, nor does it explicitly state that these glycans do not occur in human cells. (LLM, conf=0.87)
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Research works printed but not linked: 9
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The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line
Xu, X., Nagarajan, H., Lewis, N. E., Pan, S., Cai, Z., Liu, X., Chen, W., Xie, M., Wang, W., Hammond, S., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1932 · Cited by 785 · see citing works
Ghaderi, D., Zhang, M., Hurtado-Ziola, N., & Varki, A. (2012) · Biotechnology and Genetic Engineering Reviews · doi:10.5661/bger-28-147 · Cited by 320 · see citing works
Therapeutic glycoprotein production in mammalian cells
Lalonde, M.-E., & Durocher, Y. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.04.028 · Cited by 296 · see citing works
Glycoengineering in CHO cells: advances in systems biology
Tejwani, V., Andersen, M. R., Nam, J. H., & Sharfstein, S. T. (2018) · Biotechnology Journal · doi:10.1002/biot.201700234 · Cited by 70 · see citing works
- Authors
- Bosques, C. J., Collins, B. E., Meador, J. W., Sarvaiya, H., Murphy, J. L., DelloRusso, G., Bulik, D. A., Hsu, I.-H., Washburn, N., Sipsey, S. F., Myette, J. R., Raman, R., Shriver, Z., Sasisekharan, R., & Venkataraman, G.
- Affiliations
- Momenta Pharmaceuticals (United States) (US)
- Harvard–MIT Division of Health Sciences and Technology (US)
- Corresponding author
- Venkataraman, Ganesh
- ORCID
- Venue
- Nature Biotechnology
- Published
- 2010-11
- Volume
- 28
- Issue
- 11
- Pages
- 1153-1156
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- PMC
- PMC4005363
- OpenAlex
- W1547997448
- Cited by
- 159
- References
- 0
- Citations by year
- 2026: 4 · 2025: 11 · 2024: 6 · 2023: 13 · 2022: 7 · 2021: 8 · 2020: 4 · 2019: 6 · 2018: 15 · 2017: 11 · 2016: 12 · 2015: 11 · 2014: 15 · 2013: 14 · 2012: 14
- FWCI
- 4.69
- Type
- journal article
- Language
- en
- Update notices
- addendum (2011-05-06)
- Open access
- bronze
- doi.org
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- DSpace@MIT (Massachusetts Institute of Technology) (submittedVersion, cc-by-nc-sa)
- PMC
- Funders
- National Institutes of Health
- National Institute of Environmental Health Sciences (T32ES007020)
- National Center for Research Resources (P41RR018502)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Health Sciences › Medicine › Surgery › Xenotransplantation and immune response
- Keywords
- CHO cells, glycosyltransferases, protein glycosylation, anti-Neu5Gc antibodies, monoclonal antibodies, lectin binding
- MeSH terms
- Amino Acid Sequence, Animals, Antigens, Carbohydrate Conformation, Cricetulus, Disaccharides, Galactosyltransferases, Cricetinae, Humans, Molecular Sequence Data, Polysaccharides, Proteins, RNA, Messenger, Mass Spectrometry, CHO Cells, Catalytic Domain
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