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Immunofluorescence stimulated emission depletion microscopy
Open accessAbstract
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of ∼50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation.
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Cited by
A guide to super-resolution fluorescence microscopy
Schermelleh, L., Heintzmann, R., & Leonhardt, H. (2010) · Journal of Cell Biology · doi:10.1083/jcb.201002018 · Cited by 1,255
Macromolecular-scale resolution in biological fluorescence microscopy
Donnert, G., Keller, J., Medda, R., Andrei, M. A., Rizzoli, S. O., Lührmann, R., Jahn, R., Eggeling, C., & Hell, S. W. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0604965103 · Cited by 527
Self-citationStimulated Emission Depletion Microscopy
Blom, H., & Widengren, J. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00653 · Cited by 339
The molecular basis for calcium-dependent axon pathfinding
Gomez, T. M., & Zheng, J. Q. (2006) · Nature Reviews Neuroscience · doi:10.1038/nrn1844 · Cited by 330
Wurm, C. A., Neumann, D., Lauterbach, M. A., Harke, B., Egner, A., Hell, S. W., & Jakobs, S. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1107553108 · Cited by 173
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Metadata
- Authors
- Dyba, M., Jakobs, S., & Hell, S. W.
- Venue
- Nature Biotechnology
- Published
- 2003-10-19
- Volume
- 21
- Issue
- 11
- Pages
- 1303-1304
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2004879799
- Cited by
- 161
- References
- 0
- FWCI
- 5.01
- Type
- journal article
- Language
- en
- Open access
- green
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