The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo
Open accessAbstract
The small GTPase Rac induces actin polymerization, membrane ruffling and focal contact formation in cultured single cells but can either repress or stimulate motility in epithelial cells depending on the conditions. The role of Rac in collective epithelial cell movements in vivo, which are important for both morphogenesis and metastasis, is therefore difficult to predict. Recently, photoactivatable analogues of Rac (PA-Rac) have been developed, allowing rapid and reversible activation or inactivation of Rac using light. In cultured single cells, light-activated Rac leads to focal membrane ruffling, protrusion and migration. Here we show that focal activation of Rac is also sufficient to polarize an entire group of cells in vivo, specifically the border cells of the Drosophila ovary. Moreover, activation or inactivation of Rac in one cell of the cluster caused a dramatic response in the other cells, suggesting that the cells sense direction as a group according to relative levels of Rac activity. Communication between cells of the cluster required Jun amino-terminal kinase (JNK) but not guidance receptor signalling. These studies further show that photoactivatable proteins are effective tools in vivo.
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Cites
The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
Ridley, A. J., Paterson, H. F., Johnston, C. L., Diekmann, D., & Hall, A. (1992) · Cell · doi:10.1016/0092-8674(92)90164-8 · Cited by 3,652
Collective cell migration in morphogenesis, regeneration and cancer
Friedl, P., & Gilmour, D. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2720 · Cited by 2,712
Lee, T., & Luo, L. (1999) · Neuron · doi:10.1016/s0896-6273(00)80701-1 · Cited by 2,571
A genetically encoded photoactivatable Rac controls the motility of living cells
Wu, Y. I., Frey, D., Lungu, O. I., Jaehrig, A., Schlichting, I., Kuhlman, B., & Hahn, K. M. (2009) · Nature · doi:10.1038/nature08241 · Cited by 1,083
Self-citationLuo, L., Liao, Y. J., Jan, L. Y., & Jan, Y. N. (1994) · Genes & Development · doi:10.1101/gad.8.15.1787 · Cited by 983
The small GTP-binding protein rac regulates growth factor-induced membrane ruffling
Ridley, A. J., Paterson, H. F., Johnston, C. L., Diekmann, D., & Hall, A. (1992) · Trends in Cell Biology · doi:10.1016/0962-8924(92)90174-l · Cited by 755
Rørth, P. (2009) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.042308.113231 · Cited by 599
Guidance of Cell Migration by the Drosophila PDGF/VEGF Receptor
Duchek, P., Somogyi, K., Jékely, G., Beccari, S., & Rørth, P. (2001) · Cell · doi:10.1016/s0092-8674(01)00502-5 · Cited by 475
Systematic gain-of-function genetics in Drosophila
Rørth, P., Szabo, K., Bailey, A., Laverty, T., Rehm, J., Rubin, G. M., Weigmann, K., Milán, M., Benes, V., Ansorge, W., et al. (1998) · Development · doi:10.1242/dev.125.6.1049 · Cited by 462
Rho-family GTPases in cadherin-mediated cell — cell adhesion
Fukata, M., & Kaibuchi, K. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35103068 · Cited by 438
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Classifying collective cancer cell invasion
Friedl, P., Locker, J., Sahai, E., & Segall, J. E. (2012) · Nature Cell Biology · doi:10.1038/ncb2548 · Cited by 963
The Ins and Outs of the Epithelial to Mesenchymal Transition in Health and Disease
Nieto, M. A. (2011) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-092910-154036 · Cited by 720
Nonpolarized signaling reveals two distinct modes of 3D cell migration
Petrie, R. J., Gavara, N., Chadwick, R. S., & Yamada, K. M. (2012) · Journal of Cell Biology · doi:10.1083/jcb.201201124 · Cited by 375
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Metadata
- Authors
- Wang, X., He, L., Wu, Y. I., Hahn, K. M., & Montell, D. J.
- Venue
- Nature Cell Biology
- Published
- 2010-05-16
- Volume
- 12
- Issue
- 6
- Pages
- 591-597
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W1968650783
- Cited by
- 340
- References
- 33
- FWCI
- 9.57
- Type
- journal article
- Language
- en
- Open access
- green
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