The DOI matches its Crossref record; 77 of the 77 references it cites were found in a registry or an index.
Nonpolarized signaling reveals two distinct modes of 3D cell migration
Open accessAbstract
We search in this paper for context-specific modes of three-dimensional (3D) cell migration using imaging for phosphatidylinositol (3,4,5)-trisphosphate (PIP3) and active Rac1 and Cdc42 in primary fibroblasts migrating within different 3D environments. In 3D collagen, PIP3 and active Rac1 and Cdc42 were targeted to the leading edge, consistent with lamellipodia-based migration. In contrast, elongated cells migrating inside dermal explants and the cell-derived matrix (CDM) formed blunt, cylindrical protrusions, termed lobopodia, and Rac1, Cdc42, and PIP3 signaling was nonpolarized. Reducing RhoA, Rho-associated protein kinase (ROCK), or myosin II activity switched the cells to lamellipodia-based 3D migration. These modes of 3D migration were regulated by matrix physical properties. Specifically, experimentally modifying the elasticity of the CDM or collagen gels established that nonlinear elasticity supported lamellipodia-based migration, whereas linear elasticity switched cells to lobopodia-based migration. Thus, the relative polarization of intracellular signaling identifies two distinct modes of 3D cell migration governed intrinsically by RhoA, ROCK, and myosin II and extrinsically by the elastic behavior of the 3D extracellular matrix.
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Cites
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Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment
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Mechanoreciprocity in cell migration
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3D collagen alignment limits protrusions to enhance breast cancer cell persistence
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Metadata
- Authors
- Petrie, R. J., Gavara, N., Chadwick, R. S., & Yamada, K. M.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2012-04-30
- Volume
- 197
- Issue
- 3
- Pages
- 439-455
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2026446225
- Cited by
- 375
- References
- 77
- FWCI
- 15.85
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Petrie, R. J., Gavara, N., Chadwick, R. S., & Yamada, K. M. (2012). Nonpolarized signaling reveals two distinct modes of 3D cell migration. Journal of Cell Biology. https://doi.org/10.1083/jcb.201201124
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
