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The kinesin-4 protein Kif7 regulates mammalian Hedgehog signalling by organizing the cilium tip compartment
Open accessAbstract
Mammalian Hedgehog (Hh) signal transduction requires a primary cilium, a microtubule-based organelle, and the Gli-Sufu complexes that mediate Hh signalling, which are enriched at cilia tips. Kif7, a kinesin-4 family protein, is a conserved regulator of the Hh signalling pathway and a human ciliopathy protein. Here we show that Kif7 localizes to the cilium tip, the site of microtubule plus ends, where it limits cilium length and controls cilium structure. Purified recombinant Kif7 binds the plus ends of growing microtubules in vitro, where it reduces the rate of microtubule growth and increases the frequency of microtubule catastrophe. Kif7 is not required for normal intraflagellar transport or for trafficking of Hh pathway proteins into cilia. Instead, a central function of Kif7 in the mammalian Hh pathway is to control cilium architecture and to create a single cilium tip compartment, where Gli-Sufu activity can be correctly regulated.
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“β-arrestins are adaptor proteins that are recruited to the proximal C-terminus of GPR161 in a manner dependent on G protein-coupled receptor kinase 2 (GRK2)138.”
From Cellular signalling by primary cilia in development, organ function and disease (2019) · cites this paper as [138]
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“GPR161 is also removed from primary cilia in a SMO-dependent and β-arrestin-dependent manner following activation of HH signalling 28, 138.”
From Cellular signalling by primary cilia in development, organ function and disease (2019) · cites this paper as [138]
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Citations
Cites
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Items 101–200 of 302
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Cytoplasmic Cl − couples membrane remodeling to epithelial morphogenesis
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Motor Cooperation During Mitosis and Ciliogenesis
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Chong, Y. C., Mann, R. K., Zhao, C., Kato, M., & Beachy, P. A. (2015) · Genes & Development · doi:10.1101/gad.252676.114 · Cited by 35
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“Indeed, the kinked cilia and bulbous tips induced by FHDC1 overexpression are similar to the defects observed in Kif7-depleted cells (He et al., 2014) and may indicate an additional role for FHDC1 in the cilium itself.”
BackgroundCiliary Tip Signaling Compartment Is Formed and Maintained by Intraflagellar Transport
van der Burght, S. N., Rademakers, S., Johnson, J.-L., Li, C., Kremers, G., Houtsmuller, A. B., Leroux, M. R., & Jansen, G. (2020) · Current Biology · doi:10.1016/j.cub.2020.08.032 · Cited by 34
The deubiquitinating enzyme Usp14 controls ciliogenesis and Hedgehog signaling
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Altered GLI3 and FGF8 signaling underlies acrocallosal syndrome phenotypes inKif7depleted mice
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The small GTPase RSG1 controls a final step in primary cilia initiation
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“KIF7, a regulator of Shh signaling and of the cilia tip compartment (He et al., 2014), also accumulated further at ciliary tips upon SAG addition in WT and Rsg1pxb MEFs (Fig.”
BackgroundSelf-citationNew insights into the roles for DYRK family in mammalian development and congenital diseases
Yoshida, S., & Yoshida, K. (2022) · Genes & Diseases · doi:10.1016/j.gendis.2021.12.004 · Cited by 31
“Disorders in the ciliary trafficking of Hh components cause Hh signaling dysfunction.(118) Deletion of Dyrk2 induces abnormal ciliary trafficking of Hh pathway components, GLI2 and GLI3, but not SMO (Fig.”
BackgroundMouse Models of Rare Craniofacial Disorders
Achilleos, A., & Trainor, P. A. (2015) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2015.07.011 · Cited by 33
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“Kif7 localizes in the tip of the primary cilium and is believed to regulate Gli activity by controlling cilium structure [6].”
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Self-citationCEP104/FAP256 and associated cap complex maintain stability of the ciliary tip
Legal, T., Parra, M., Tong, M., Black, C. S., Joachimiak, E., Valente-Paterno, M., Lechtreck, K. F., Gaertig, J., & Bui, K. H. (2023) · The Journal of Cell Biology · doi:10.1083/jcb.202301129 · Cited by 29
“Kinesins from the kinesin-4, -8, and -13 subfamilies localize to the ciliary tips, where they regulate microtubule length (He et al., 2014; Niwa et al., 2012; Piao et al., 2009; Schwarz et al., 2017; Vasudevan et al., 2015; Wang et al., 2013).”
Suzuki, A., Li, A., Gajera, M. V., Abdallah, N., Zhang, M., Zhao, Z., & Iwata, J. (2019) · BMC Medical Genomics · doi:10.1186/s12920-019-0546-z · Cited by 31
Kinesin superfamily protein 21B acts as an oncogene in non-small cell lung cancer
Sun, Z., Pan, F., Shao, J., Yan, Q.-Q., Lu, L., & Zhang, N. (2020) · Cancer Cell International · doi:10.1186/s12935-020-01323-7 · Cited by 29
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“In the absence of Hh signals, Cos-2 binds to the Hh/Shh effector Ci/Gli and tethers it to microtubules together with Fused, Suppressor of Fused and several kinases (He et al., 2014; Robbins et al., 1997).”
BackgroundThorpe, S. D., Gambassi, S., Thompson, C. L., Chandrakumar, C., Santucci, A., & Knight, M. M. (2017) · Journal of Cellular Physiology · doi:10.1002/jcp.25839 · Cited by 29
“Upon activation of theHedgehog signaling pathway, Gli proteins are trafficked to the ciliary tip where a complex of proteins, including the kinesin-4 protein Kif7, is localized and regulates Gli activity (He et al., 2014).”
BackgroundKIF14 controls ciliogenesis via regulation of Aurora A and is important for Hedgehog signaling
Pejšková, P., Reilly, M. L., Binó, L., Bernatík, O., Dolanská, L., Ganji, R. S., Zdráhal, Z., Benmerah, A., & Čajánek, L. (2020) · The Journal of Cell Biology · doi:10.1083/jcb.201904107 · Cited by 31
“…1999; Morris and Scholey, 1997), KIF7 seems to organize the HH signaling platform at the tip of the primary cilium (Endoh-Yamagami et al., 2009; He et al., 2014), and it has been proposed that KIF24 negatively regulates the early steps of primary cilium formation (Kobayashi et al., 2011) and…”
InfluentialRabl2 GTP hydrolysis licenses BBSome‐mediated export to fine‐tune ciliary signaling
Duan, S., Li, H., Zhang, Y., Yang, S., Chen, Y., Qiu, B., Huang, C., Wang, J., Li, J., Zhu, X., et al. (2020) · The EMBO Journal · doi:10.15252/embj.2020105499 · Cited by 27
“0.27 l m/s, respectively, similar to those of reported IFT particles (He et al , 2014; Ishikawa & Marshall, 2015).”
InfluentialMutations in KIF7 implicated in idiopathic scoliosis in humans and axial curvatures in zebrafish
Terhune, E., Cuevas, M. T., Monley, A. M., Wethey, C. I., Chen, X., Cattell, M. V., Bayrak, M. N., Bland, M. R., Sutphin, B. S., Trahan, G. D., et al. (2020) · Human Mutation · doi:10.1002/humu.24162 · Cited by 29
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“GLI2, a transcriptional activator of the pathway, becomes enriched at the distal tip of cilia (35), as does the atypical kinesin KIF7, which regulates the structure of the ciliary tip compartment and participates in HH signaling (36, 37).”
BackgroundZhang, Y., Chen, W., Zeng, W., Lu, Z., & Zhou, X. (2020) · Cell Death and Disease · doi:10.1038/s41419-020-03214-1 · Cited by 28
Choi, H., Shin, J. H., Kim, E. S., Park, S. J., Bae, I., Jo, Y. K., Jeong, I. Y., Kim, H.-J., Lee, Y., Park, H. C., et al. (2016) · PLoS ONE · doi:10.1371/journal.pone.0168025 · Cited by 28
Primary Cilia in Cystic Kidney Disease
Avasthi, P. P., Maser, R. L., & Tran, P. V. (2017) · Results and problems in cell differentiation · doi:10.1007/978-3-319-51436-9_11 · Cited by 29
Lewis, T. R., Kundinger, S. R., Pavlovich, A. L., Bostrom, J. R., Link, B. A., & Besharse, J. C. (2017) · Developmental Biology · doi:10.1016/j.ydbio.2017.03.019 · Cited by 28
“While genetic loss of mouse Cos2/Kif7 does not affect IFT, it does lead to ectopic activation of Hh signaling through mislocalization of Gli proteins throughout the cilia (He et al., 2014).”
BackgroundMethodInfluentialMechanistic insights into skeletal development gained from genetic disorders
Yip, R. K. H., Chan, D. T.-M., & Cheah, K. S. E. (2019) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2019.02.002 · Cited by 26
Hedgehog pathway modulation by glypican 3-conjugated heparan sulfate
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“The phenotype of GPC3 inhibition, characterized by normal Smo entry into cilia but loss of Gli recruitment, resembles that caused by the loss of the KIF7 kinesin (Endoh-Yamagami et al., 2009; He et al., 2014) or one of its upstream regulators, such as DLG5 (Chong et al.”
BackgroundCilia regulate meiotic recombination in zebrafish
Xie, H., Wang, X., Jin, M., Li, L., Zhu, J., Kang, Y., Chen, Z., Sun, Y., & Zhao, C. (2022) · Journal of Molecular Cell Biology · doi:10.1093/jmcb/mjac049 · Cited by 26
Mechanisms of Regulation in Intraflagellar Transport
Mul, W., Mitra, A., & Peterman, E. J. G. (2022) · Cells · doi:10.3390/cells11172737 · Cited by 28
“For instance, in mammalian cells, the non-motile kinesin-4, Kif7, a passive cargo of IFT trains, localizes and stabilizes axonemal tips [145,146].”
BackgroundHedgehog Signaling Regulates the Ciliary Transport of Odorant Receptors in Drosophila
Sanchez, G. M., Alkhori, L., Hatano, E., Schultz, S. W., Kuzhandaivel, A., Jafari, S., Granseth, B., & Alenius, M. (2016) · Cell Reports · doi:10.1016/j.celrep.2015.12.059 · Cited by 30
“Interestingly, the vertebrate Cos2 homolog Kif7 organizes the distal compartment of vertebrate primary cilia (He et al., 2014).”
BackgroundResultControl of Hedgehog Signalling by the Cilia-Regulated Proteasome
Gerhardt, C., Wiegering, A., Leu, T., & Rüther, U. (2016) · Journal of Developmental Biology · doi:10.3390/jdb4030027 · Cited by 26
“An additional player in regulating GLI processing and activity is the kinesin family member 7 (KIF7) protein which localises along the entire cilium [31–35].”
BackgroundDaggubati, V., Hochstelter, J., Bommireddy, A., Choudhury, A., Krup, A. L., Kaur, P., Tong, P., Li, A., Xu, L., Reiter, J. F., et al. (2021) · Journal of Clinical Investigation · doi:10.1172/jci141171 · Cited by 26
“the majority of UW228 cells lacked primary cilia (Supplemental Figure 1A), and ONS76 cells expressed shortened cilia (Supplemental Figure 1B), a marker of reduced HH signal transduction (13-15).”
BackgroundBarny, I., Perrault, I., Michel, C., Goudin, N., Defoort‐Dhellemmes, S., Ghazi, I., Kaplan, J., Rozet, J., & Gérard, X. (2019) · Genes · doi:10.3390/genes10050368 · Cited by 26
Actin-based regulation of ciliogenesis – The long and the short of it
Copeland, J. W. R. (2019) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2019.12.005 · Cited by 27
The Biology of Ciliary DynamicsPreprint
Hsu, K., Chuang, J.-Z., & Sung, C.-H. (2017) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a027904 · Cited by 25
“Ultrastructural studies showed that the primary cilia in several mouse disease models are normal in length, but not in shape (Davis et al. 2007; Tran et al. 2008; Bielas et al. 2009; Jacoby et al. 2009; Goggolidou et al. 2014; He et al. 2014; Siller et al. 2015).”
Getwan, M., Hoppmann, A., Schlosser, P., Grand, K., Song, W., Diehl, R., Schroda, S., Heeg, F., Deutsch, K., Hildebrandt, F., et al. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2106770118 · Cited by 25
“Knockout of Kif7 affects PTMs mainly at the tip of the cilium (116, 117).”
BackgroundLin, W., Lin, X., Fu, S., Li, Y., Tang, C., Gao, Y., Chen, H., & Ge, Z.-Z. (2018) · Molecular Carcinogenesis · doi:10.1002/mc.22812 · Cited by 24
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“regulation and cell-cell adhesion [10, 11, 45-51].”
BackgroundRegulation of Hedgehog Signal Transduction by Ubiquitination and Deubiquitination
Zhang, Q., & Jiang, J. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms222413338 · Cited by 23
Cytoskeletal regulation of a transcription factor by DNA mimicry via coiled-coil interactions
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Self-citationKif7 keeps cilia tips in shape
Pedersen, L. B., & Akhmanova, A. S. (2014) · Nature Cell Biology · doi:10.1038/ncb2997 · Cited by 23
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“18 Typically, these mutations cause either RP or macular degeneration.”
The multifaceted roles of microtubule-associated proteins in the primary cilium and ciliopathies
Deretic, J., Odabasi, E., & Firat‐Karalar, E. N. (2023) · Journal of Cell Science · doi:10.1242/jcs.261148 · Cited by 22
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The nephronophthisis-related gene ift-139 is required for ciliogenesis in Caenorhabditis elegans
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Primary Ciliary Signaling in the Skin—Contribution to Wound Healing and Scarring
Hosio, M., Jaks, V., Lagus, H., Vuola, J., Ogawa, R., & Kankuri, E. M. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.578384 · Cited by 22
“KIF7, an atypical kinesin, is also involved in the transport and correct processing of the GLI proteins at the tips of cilia, potentially via modulating by modulating the ciliary architecture (Liem et al., 2009; He et al., 2014).”
BackgroundComposition, structure and function of the eukaryotic flagellum distal tip
Croft, J. T., Zabeo, D., Subramanian, R., & Höög, J. L. (2018) · Essays in Biochemistry · doi:10.1042/ebc20180032 · Cited by 25
Self-citationSaunders, H. A. J., van den Berg, C. M., Hoogebeen, R., Schweizer, D., Stecker, K. E., Roepman, R., Howes, S. C., & Akhmanova, A. S. (2025) · Nature Structural & Molecular Biology · doi:10.1038/s41594-025-01483-y · Cited by 20
“For example, CEP104 and CSPP1 might counteract MT-depolymerizing factors, such as kinesin 13, kinesin 18 KIF19A or kinesin 4 KIF7, which are known to act at ciliary tips 58–60 .”
Yue, Y., Engelke, M. F., Blasius, T. L., & Verhey, K. J. (2021) · Molecular Biology of the Cell · doi:10.1091/mbc.e21-04-0215 · Cited by 23
“KIF7 has also been suggested to regulate the length of the primary cilium and organize the cilium tip based on its ability to bind selectively to the plus ends of microtubules in in vitro assays (He et al., 2014; Jiang et al., 2019).”
BackgroundInfluentialBlasius, T. L., Yue, Y., Prasad, R., Liu, X., Gennerich, A., & Verhey, K. J. (2021) · Journal of Cell Science · doi:10.1242/jcs.258464 · Cited by 20
Conkar, D., Odabasi, E., Deretic, J., Batman, U., Frikstad, K.-A. M., Patzke, S., & Firat‐Karalar, E. N. (2023) · Journal of Cell Science · doi:10.1242/jcs.260327 · Cited by 20
The loss of Hh responsiveness by a non-ciliary Gli2 variant
Liu, J., Zeng, H., & Liu, A. (2015) · Development · doi:10.1242/dev.119669 · Cited by 21
“However, this could prove to be difficult because Gli2 appears to dynamically traffic through a special ciliary tip compartment, thus simply directing Gli2ΔCLR localization to the ciliummay not be sufficient to restore its response to Hh signaling (He et al., 2014).”
BackgroundIncreased KIF21B expression is a potential prognostic biomarker in hepatocellular carcinoma
Zhao, H., Dong, B.-L., Zhang, M., Dong, X., He, Y., Chen, S.-Y., Wu, B., & Yang, X. (2020) · World Journal of Gastrointestinal Oncology · doi:10.4251/wjgo.v12.i3.276 · Cited by 20
Intraflagellar Transport Complex B Proteins Regulate the Hippo Effector Yap1 during Cardiogenesis
Peralta, M., López, L. O., Jeřábková, K., Lucchesi, T., Vitre, B., Han, D., Guillemot, L., Dingare, C., Sumara, I., Mercader, N., et al. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107932 · Cited by 21
“Arl13b-GFP Borovina et al., 2010 ZFIN ID: ZDB-ALT-100721-1 Zebrafish: tcf21:NLS-EGFP Kikuchi et al., 2011 ZFIN ID: ZDB-ALT-110914-2 Mice: Ift20null/+ (C57BL background) Jonassen et al., 2008 MGI: 12565 Mice: PGK-Cre Lallemand et al., 1998 PMID: 9608738 Mice: Ift88null/+ (6JRj background) Haycraft et al., 2007 MGI:5505…”
InfluentialThe Role of Hedgehog Signalling in the Formation of the Ventricular Septum
Wiegering, A., Rüther, U., & Gerhardt, C. (2017) · Journal of Developmental Biology · doi:10.3390/jdb5040017 · Cited by 20
Systematic analysis of cilia characteristics and Hedgehog signaling in five immortal cell lines
Gómez, A. E., Christman, A. K., Weghe, J. C. V. D., Finn, M., & Doherty, D. (2022) · PLoS ONE · doi:10.1371/journal.pone.0266433 · Cited by 20
“In concert, GLI2/3, KIF7, and Suppressor of Fused (SUFU) localization to the ciliary tip increases, and is associated with reduced GLI2/3 cleavage [9, 10].”
BackgroundEfficient live fluorescence imaging of intraflagellar transport in mammalian primary cilia
Ishikawa, H., & Marshall, W. F. (2015) · Methods in cell biology · doi:10.1016/bs.mcb.2015.01.002 · Cited by 22
“Because IFT moves at a speed of approximately 1 μm/s inside of cilia in both directions in mammalian cells (He et al., 2014; Ishikawa et al., 2014), IFT imaging needs to acquire images at a fast enough rate that the motion of the trains does not cause image blurring.”
BackgroundNovas, R., Cárdenas-Rodríguez, M., Lepanto, P., Fabregat, M., Rodao, M., Fariello, M. I., Ramos, M., Davison, C., Casanova, G., Alfaya, L., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-21329-6 · Cited by 18
Hu, Z., Liang, Y., Meng, D., Wang, L., & Pan, J. (2015) · International review of cytology · doi:10.1016/bs.ircmb.2015.01.008 · Cited by 21
Taguchi, S., Nakano, J., Imasaki, T., Kita, T., Saijo‐Hamano, Y., Sakai, N., Shigematsu, H., Okuma, H., Shimizu, T., Nitta, E., et al. (2022) · eLife · doi:10.7554/elife.77877 · Cited by 18
Low expression of KIF7 indicates poor prognosis in epithelial ovarian cancer
Yao, Y., Liu, L., He, W., Lin, X., Zhang, X., Lin, Z., Zeng, Z., & Guo, S. (2019) · Cancer Biomarkers · doi:10.3233/cbm-190328 · Cited by 18
“1 and is a kinesin-4 family member that has been indicated to have critical roles in Hedgehog (Hh) signaling during embryonic development [8,9].”
Exploring Sonic Hedgehog Cell Signaling in Neurogenesis: Its Potential Role in Depressive Behavior
Rana, T., Behl, T., Sehgal, A., Sachdeva, M., Mehta, V., Sharma, N., Singh, S., & BUNGAU, S. G. (2021) · Neurochemical Research · doi:10.1007/s11064-021-03307-z · Cited by 20
Sufu and Kif7 in limb patterning and development
Zhulyn, O., & Hui, C. (2015) · Developmental Dynamics · doi:10.1002/dvdy.24249 · Cited by 19
“Studies in the skin, chondrocytes, and neural tube as well as in vitro have demonstrated that Kif7 promotes pathway activation by facilitating the dissociation of Sufu-Gli complexes (He et al., 2014; Hsu et al., 2011; Humke et al., 2010; Law et al., 2012; Li et al., 2012; Maurya et al., 2013).”
BackgroundKIF7 attenuates prostate tumor growth through LKB1-mediated AKT inhibition
Wong, K. Y., Liu, J., & Chan, K. W. (2017) · Oncotarget · doi:10.18632/oncotarget.17421 · Cited by 17
“However, Varjosalo et al demonstrated that unlike Costal2 from Drosophila Kif7 has no effect on Hh pathway in NIH-3T3 cells [38].”
BackgroundInfluentialHor, C. H. H., & Goh, E. L. K. (2019) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2019.07.009 · Cited by 19
Beyond cyclopamine: Targeting Hedgehog signaling for cancer intervention
Baby, B. T., Kulkarni, A. M., Gayam, P. K. R., Harikumar, K. B., & Aranjani, J. M. (2024) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2024.109952 · Cited by 17
Hedgehog pathway, cell cycle, and primary cilium
Zhuang, T. (2025) · Cell Death Discovery · doi:10.1038/s41420-025-02605-7 · Cited by 17
“2) where the disassociation of the Sufu-Gli complex and their activity can be appropriately regulated [247].”
Primary cilia function as hubs for signal transduction
Li, B., He, Y., & Yang, Z. (2025) · Cell & Bioscience · doi:10.1186/s13578-025-01471-1 · Cited by 17
“At the ciliary tip, Kinesin Family Member 7 (Kif7) organizes the ciliary compartment and regulates GLI activation, thereby ensuring precise control of Hh signal transduction [81, 82].”
Primary Cilia: A Cellular Regulator of Articular Cartilage Degeneration
Zhou, H., Wu, S., Ling, H., Zhang, C., & Kong, Y. (2022) · Stem Cells International · doi:10.1155/2022/2560441 · Cited by 16
“In this regard, primary cilia are necessary for both ligand Indian hedgehog(Ihh-) mediated and mechanical loading-mediated Hh signaling activation in chondrocytes [71, 72].”
BackgroundTTBK2 controls cilium stability by regulating distinct modules of centrosomal proteins
Nguyen, A. V., & Goetz, S. C. (2022) · Molecular Biology of the Cell · doi:10.1091/mbc.e22-08-0373 · Cited by 17
“In addition to undermodification of tubulin being associated with instability of the ciliary axoneme (He et al., 2014), alterations to actin dynamics are also linked to ciliary breakage and shedding (Nager et al., 2017; Loukil et al., 2021).”
BackgroundARL3, a small GTPase with a functionally conserved role in primary cilia and immune synapses
Powell, L., Samarakoon, Y., Ismail, S., & Sayer, J. A. (2019) · Small GTPases · doi:10.1080/21541248.2019.1703466 · Cited by 17
Lai, F. P.-L., Li, Z., Zhou, T., Leung, A. O.-W., Lau, S.-T., Lui, K. N.-C., Wong, W. Y.-M., Sham, P. C., Hui, C., & Ngan, E. (2021) · Science Advances · doi:10.1126/sciadv.abf7472 · Cited by 14
“The kinesin motor protein Kif7 is one of the key molecules for Hedgehog signaling; it may promote ciliary trafficking and accumulation of Gli at the cilium tip and induce the microtubule-dependent translocation of Gli to the nucleus (10) and/or organize and stabilize the cilia tip (11).”
BackgroundKu, P.-I., Sreeja, J. S., Chadha, A., Williams, D. S., Engelke, M. F., & Subramanian, R. (2025) · Science Advances · doi:10.1126/sciadv.adt5439 · Cited by 15
Self-citationThe utility of stem cells in pediatric urinary bladder regeneration
Iannaccone, P. M., Galat, V., Bury, M. I., Ma, Y. C., & Sharma, A. K. (2017) · Pediatric Research · doi:10.1038/pr.2017.229 · Cited by 14
Jana, S. C., Dutta, P., Jain, A., Singh, A., Adusumilli, L., Girotra, M., Kumari, D., Shirolikar, S. M., & Ray, K. (2021) · PLoS Genetics · doi:10.1371/journal.pgen.1009752 · Cited by 14
“A separate study further suggested that the kinesin-family motor Costal2/Kif7, which is immotile and does not bind to either Smo or Gli, establishes the signalling-competent compartment at the ciliary tip [15].”
BackgroundHedgehog Signaling Pathway in Fibrosis and Targeted Therapies
Hu, Y., Peng, L., Zhuo, X., Yang, C., & Zhang, Y. (2024) · Biomolecules · doi:10.3390/biom14121485 · Cited by 17
“In the active state, SMO accumulates throughout the membrane of the PC. Kif7 aids in the movement of the GLI/SUFU complex through the PC [40].”
Philbrook, A., O’Donnell, M. P., Grunenkovaite, L., & Sengupta, P. (2024) · PLoS Biology · doi:10.1371/journal.pbio.3002892 · Cited by 13
Non-canonical CDK6 activity promotes cilia disassembly by suppressing axoneme polyglutamylation
He, K., Sun, X., Chen, C., Luc, S., Hao, J., Zhang, Y., Huang, Y., Ji, B., Ku, P.-I., Subramanian, R., et al. (2024) · The Journal of Cell Biology · doi:10.1083/jcb.202405170 · Cited by 14
Self-citationThe flagellar length control system: exploring the physical biology of organelle size
Marshall, W. F. (2023) · Physical Biology · doi:10.1088/1478-3975/acb18d · Cited by 14
Li, Q., Liu, Y., Wu, J., Zhu, Z., Fan, J., Zhai, L., Wang, Z., Du, G., Zhang, L., Hu, J., et al. (2024) · Leukemia · doi:10.1038/s41375-024-02313-8 · Cited by 12
“Upon activation, core pathway components, including the SUFU-GLI complex, translocate to the distal cilia tips, where the complex dissociates, allowing GLIs enter the nucleus and initiate downstream genes transcription [4, 6 – 8].”
BackgroundResultInfluentialChen, C., Xu, Q., Zhang, Y., Davies, B. A., Huang, Y., Katzmann, D. J., Harris, P. C., Hu, J., & Ling, K. (2021) · Science Advances · doi:10.1126/sciadv.abe3401 · Cited by 13
Sun, Y., Tian, Y., Wang, G., Zhao, S., Han, B. R., Li, Y., & Jiang, C. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18030235 · Cited by 12
“Mammalian Hedgehog signal transduction requires a microtubule-based organelle, where glioma-associated oncogene homologs (GLIs) activity can be correctly regulated [38].”
BackgroundColes, G. L., Baglia, L. A., & Ackerman, K. G. (2015) · PLoS Genetics · doi:10.1371/journal.pgen.1005525 · Cited by 12
“Kinesin family member 7 (KIF7) is a microtubule interacting protein that functions to regulate Hh signaling by maintaining the architecture of the primary cilium [11], a complex microtubule based organelle with many signal transduction functions [12,13].”
BackgroundInfluentialAxonemal microtubule dynamics in the assembly and disassembly of cilia
Zhang, Y., He, M., & Pan, J. (2025) · Biochemical Society Transactions · doi:10.1042/bst20240688 · Cited by 12
“Genetic inactivation of KIF7 in cultured fibroblasts results in slightly longer and less stable cilia [32,33].”
InfluentialSelf-citationJiang, H., Liu, S., Cheung, M. H., Amin, A., & Liang, C. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.590449 · Cited by 9
“For ciliary modification assay, fluorescence intensity was measured using line-scan-based analysis in Image J, as previously described (He et al., 2014).”
MethodSonic Hedgehog activates prostaglandin signaling to stabilize primary cilium length
Ansari, S. S., Dillard, M. E., Zhang, Y., Austria, M. A., Boatwright, N., Shelton, E. L., Stewart, D. P., Johnson, A. J., Wang, C. E., Young, B. M., et al. (2024) · The Journal of Cell Biology · doi:10.1083/jcb.202306002 · Cited by 12
Location, location, and location: compartmentalization of Hedgehog signaling at primary cilia
Pusapati, G. V., & Rohatgi, R. (2014) · The EMBO Journal · doi:10.15252/embj.201489294 · Cited by 11
“He et al (2014) analyzed the biochemical properties of KIF7 toward microtubules using a purified N-terminal fragment of the protein containing the motor domain and the first coiled-coil segment.”
Tiryaki, F., Deretic, J., & Firat‐Karalar, E. N. (2022) · FEBS Journal · doi:10.1111/febs.16367 · Cited by 11
Metadata
- Authors
- He, M., Subramanian, R., Bangs, F., Omelchenko, T., Liem Jr, K. F., Kapoor, T. M., & Anderson, K. V.
- Affiliations
- Memorial Sloan Kettering Cancer Center (US)
- Rockefeller University (US)
- Corresponding author
- Anderson, Kathryn V.
- Venue
- Nature Cell Biology
- Published
- 2014-06-22
- Volume
- 16
- Issue
- 7
- Pages
- 663-672
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- PMC
- PMC4085576
- OpenAlex
- W2083616180
- Cited by
- 302
- References
- 55
- Citations by year
- 2026: 14 · 2025: 20 · 2024: 20 · 2023: 13 · 2022: 30 · 2021: 26 · 2020: 26 · 2019: 27 · 2018: 31 · 2017: 34 · 2016: 22 · 2015: 32 · 2014: 7
- FWCI
- 20.74
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Cancer Institute (P30CA008748)
- National Institute of General Medical Sciences (R01GM065933, GM65933)
- National Institute of Neurological Disorders and Stroke (NS044385, R01NS044385)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Hedgehog Signaling Pathway Studies
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Keywords
- KIF7, kinesin-4, Hedgehog signaling, primary cilia, microtubule plus ends, ciliopathies, intraflagellar transport, ciliary trafficking
- MeSH terms
- Animals, Cell Line, Cells, Cultured, Cilia, Fibroblasts, Humans, Microtubules, Mutation, Protein Binding, Recombinant Proteins, Signal Transduction, Kinesins, Protein Transport, NIH 3T3 Cells, Mice, Hedgehog Proteins, Axoneme, HEK293 Cells
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