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Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells
Open accessAbstract
The use of human pluripotent stem cells for in vitro disease modelling and clinical applications requires protocols that convert these cells into relevant adult cell types. Here, we report the rapid and efficient differentiation of human pluripotent stem cells into vascular endothelial and smooth muscle cells. We found that GSK3 inhibition and BMP4 treatment rapidly committed pluripotent cells to a mesodermal fate and subsequent exposure to VEGF-A or PDGF-BB resulted in the differentiation of either endothelial or vascular smooth muscle cells, respectively. Both protocols produced mature cells with efficiencies exceeding 80% within six days. On purification to 99% via surface markers, endothelial cells maintained their identity, as assessed by marker gene expression, and showed relevant in vitro and in vivo functionality. Global transcriptional and metabolomic analyses confirmed that the cells closely resembled their in vivo counterparts. Our results suggest that these cells could be used to faithfully model human disease.
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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310
Warren, L., Manos, P. D., Ahfeldt, T., Loh, Y.-H., Li, H., Lau, F., Ebina, W., Mandal, P. K., Smith, Z. D., Meissner, A., et al. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.08.012 · Cited by 2,662
Self-citationA ROCK inhibitor permits survival of dissociated human embryonic stem cells
Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J. B., Nishikawa, S., Nishikawa, S.-I., Muguruma, K., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1310 · Cited by 2,055
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Noor, N., Shapira, A., Edri, R., Gal, I., Wertheim, L., & Dvir, T. (2019) · Advanced Science · doi:10.1002/advs.201900344 · Cited by 1,042
Human blood vessel organoids as a model of diabetic vasculopathy
Wimmer, R. A., Leopoldi, A., Aichinger, M., Wick, N., Hantusch, B., Novatchkova, M., Taubenschmid, J., Hämmerle, M., Esk, C., Bagley, J. A., et al. (2019) · Nature · doi:10.1038/s41586-018-0858-8 · Cited by 803
Vascular Smooth Muscle Cells and Arterial Stiffening: Relevance in Development, Aging, and Disease
Lacolley, P., Regnault, V., Segers, P., & Laurent, S. (2017) · Physiological Reviews · doi:10.1152/physrev.00003.2017 · Cited by 734
Cardioids reveal self-organizing principles of human cardiogenesis
Hofbauer, P., Jahnel, S. M., Papai, N., Giesshammer, M., Deyett, A., Schmidt, C., Penc, M., Tavernini, K., Grdseloff, N., Meledeth, C., et al. (2021) · Cell · doi:10.1016/j.cell.2021.04.034 · Cited by 588
Gupta, R. M., Hadaya, J., Trehan, A., Zekavat, S. M., Roselli, C., Klarin, D., Emdin, C. A., Hilvering, C. R. E., Bianchi, V., Mueller, C., et al. (2017) · Cell · doi:10.1016/j.cell.2017.06.049 · Cited by 450
Metadata
- Authors
- Patsch, C., Challet-Meylan, L., Thoma, E. C., Urich, E., Heckel, T., O’Sullivan, J. F., Grainger, S. J., Kapp, F. G., Sun, L., Christensen, K., Xia, Y., Florido, M. H. C., He, W., Pan, W., Prummer, M., Warren, C. R., Jakob-Roetne, R., Certa, U., Jagasia, R., Freskgård, P.-O., Adatto, I., Kling, D., Huang, P., Zon, L. I., Chaikof, E. L., Gerszten, R. E., Graf, M., Iacone, R., & Cowan, C. A.
- Venue
- Nature Cell Biology
- Published
- 2015-07-27
- Volume
- 17
- Issue
- 8
- Pages
- 994-1003
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W1563180177
- Cited by
- 629
- References
- 3
- FWCI
- 16.47
- Type
- journal article
- Language
- en
- Open access
- green
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- The paper
- Patsch, C., Challet-Meylan, L., Thoma, E. C., Urich, E., Heckel, T., O’Sullivan, J. F., Grainger, S. J., Kapp, F. G., Sun, L., Christensen, K., Xia, Y., Florido, M. H. C., He, W., Pan, W., Prummer, M., Warren, C. R., Jakob-Roetne, R., Certa, U., Jagasia, R., . . . Cowan, C. A. (2015). Generation of vascular endothelial and smooth muscle cells from human pluripotent stem cells. Nature Cell Biology. https://doi.org/10.1038/ncb3205
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
