The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Tropomyosin controls sarcomere-like contractions for rigidity sensing and suppressing growth on soft matrices
Open accessAbstract
Cells test the rigidity of the extracellular matrix by applying forces to it through integrin adhesions. Recent measurements show that these forces are applied by local micrometre-scale contractions, but how contraction force is regulated by rigidity is unknown. Here we performed high temporal- and spatial-resolution tracking of contractile forces by plating cells on sub-micrometre elastomeric pillars. We found that actomyosin-based sarcomere-like contractile units (CUs) simultaneously moved opposing pillars in net steps of ∼2.5 nm, independent of rigidity. What correlated with rigidity was the number of steps taken to reach a force level that activated recruitment of α-actinin to the CUs. When we removed actomyosin restriction by depleting tropomyosin 2.1, we observed larger steps and higher forces that resulted in aberrant rigidity sensing and growth of non-transformed cells on soft matrices. Thus, we conclude that tropomyosin 2.1 acts as a suppressor of growth on soft matrices by supporting proper rigidity sensing.
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Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Direct observation of kinesin stepping by optical trapping interferometry
Svoboda, K., Schmidt, C. F., Schnapp, B. J., & Block, S. M. (1993) · Nature · doi:10.1038/365721a0 · Cited by 1,910
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Cited by
Integrins as biomechanical sensors of the microenvironment
Kechagia, J. Z., Ivaska, J., & Roca-Cusachs, P. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0134-2 · Cited by 1,311
Self-citationJanmey, P. A., Fletcher, D. A., & Reinhart-King, C. A. (2020) · Physiological Reviews · doi:10.1152/physrev.00013.2019 · Cited by 422
Steps in Mechanotransduction Pathways that Control Cell Morphology
Wolfenson, H., Yang, B., & Sheetz, M. P. (2019) · Annual Review of Physiology · doi:10.1146/annurev-physiol-021317-121245 · Cited by 267
Self-citationMicrotubules tune mechanosensitive cell responses
Seetharaman, S., Vianay, B., Roca, V., Farrugia, A. J., De Pascalis, C., Boëda, B., Dingli, F., Loew, D., Vassilopoulos, S., Bershadsky, A., et al. (2022) · Nature Materials · doi:10.1038/s41563-021-01108-x · Cited by 210
Metadata
- Authors
- Wolfenson, H., Meacci, G., Liu, S., Stachowiak, M. R., Iskratsch, T., Ghassemi, S., Roca-Cusachs, P., O’Shaughnessy, B., Hone, J., & Sheetz, M. P.
- Venue
- Nature Cell Biology
- Published
- 2015-11-30
- Volume
- 18
- Issue
- 1
- Pages
- 33-42
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2205358880
- Cited by
- 202
- References
- 4
- FWCI
- 8.94
- Type
- journal article
- Language
- en
- Open access
- green
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