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Cited by 181Microtubule and mitosis dynamicsCellular Mechanics and InteractionsCancer Treatment and Pharmacology

Self-repair promotes microtubule rescue

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Aumeier, Charlotte, Schaedel, Laura, Gaillard, Jérémie, John, Karin, Blanchoin, Laurent, Théry, Manuel·2016·Nature Cell Biology
DOI 10.1038/ncb3406PMC PMC5045721
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Abstract

The dynamic instability of microtubules is characterized by slow growth phases stochastically interrupted by rapid depolymerizations called catastrophes. Rescue events can arrest the depolymerization and restore microtubule elongation. However, the origin of these rescue events remains unexplained. Here we show that microtubule lattice self-repair, in structurally damaged sites, is responsible for the rescue of microtubule growth. Tubulin photo-conversion in cells revealed that free tubulin dimers can incorporate along the shafts of microtubules, especially in regions where microtubules cross each other, form bundles or become bent due to mechanical constraints. These incorporation sites appeared to act as effective rescue sites ensuring microtubule rejuvenation. By securing damaged microtubule growth, the self-repair process supports a mechanosensitive growth by specifically promoting microtubule assembly in regions where they are subjected to physical constraints.

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  • Such regional variation is consistent with previous studies and suggests that lattice conformation can be locally influenced by lattice-binding microtubule-associated proteins, post-translational modifications, mechanical stress, or repair processes.

    “Such regional variation is consistent with previous studies58and suggest that lattice conformation can be locally influenced by lattice-binding microtuble-associated proteins (MAPs)59–61, post-translational modifications, mechanical stress50,51, or repair processes62–66.”

    From Ghost Neuron preparation unlocks high-resolution structural mapping of intracellular architecture in axons (2026) · cites this paper as [64]

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Authors
Aumeier, C., Schaedel, L., Gaillard, J., John, K., Blanchoin, L., & Théry, M.
Affiliations
Centre National de la Recherche Scientifique (FR)
Corresponding author
  • Blanchoin, Laurent
  • Théry, Manuel
ORCID
Venue
Nature Cell Biology
Publisher
Springer Science and Business Media LLC
Published
2016-09-12
Volume
18
Issue
10
Pages
1054-1064
ISSN
  • 1465-7392 (ISSN-L)
  • 1476-4679
DOI
10.1038/ncb3406
PMC
PMC5045721
OpenAlex
W2519267297
Cited by
181
References
1
Citations by year
2026: 13 · 2025: 8 · 2024: 16 · 2023: 25 · 2022: 20 · 2021: 17 · 2020: 18 · 2019: 25 · 2018: 26 · 2017: 7 · 2016: 6
FWCI
12.19
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature Cell Biology (publishedVersion)
  • PubMed (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • HAL (submittedVersion)
Funders
Agence Nationale de la Recherche (ANR-12-BSV5-0014)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Health Sciences › Medicine › Oncology › Cancer Treatment and Pharmacology
Keywords
microtubule rescue, microtubule dynamic instability, microtubule catastrophe, microtubule bundling, microtubule bending, microtubule assembly
MeSH terms
Animals, Cell Membrane, Cells, Cultured, Microtubule-Associated Proteins, Microtubules, Photolysis, Tubulin, Rats, Focal Adhesion Kinase 1

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