The exocyst is a Ral effector complex
Abstract
Delivery of cytoplasmic vesicles to discrete plasma-membrane domains is critical for establishing and maintaining cell polarity, neurite differentiation and regulated exocytosis. The exocyst is a multisubunit complex required for vectorial targeting of a subset of secretory vesicles. Mechanisms that regulate the activity of this complex in mammals are unknown. Here we show that Sec5, an integral component of the exocyst, is a direct target for activated Ral GTPases. Ral GTPases regulate targeting of basolateral proteins in epithelial cells, secretagogue-dependent exocytosis in neuroendocrine cells and assembly of exocyst complexes. These observations define Ral GTPases as critical regulators of vesicle trafficking.
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The interaction of RalA with Sec5 and Exo84 is thought to promote complex assembly, vesicle exocytosis, and membrane addition.
“this interaction is thought to promote complex assembly, vesicle exocytosis, and membrane addition (Moskalenko et al, 2002(Moskalenko et al, , 2003;;Sugihara et al, 2002;Fukai et al, 2003;Mott et al, 2003;Wang et al, 2004;Jin et al, 2005;Hase et al, 2009).”
From Ral mediates activity‐dependent growth of postsynaptic membranes via recruitment of the exocyst (2013) · cites this paper as Moskalenko et al, 2002
RalA binds directly to the exocyst members Sec5 and Exo84.
“RalA binds directly to the exocyst members Sec5 and Exo84”
From Ral mediates activity‐dependent growth of postsynaptic membranes via recruitment of the exocyst (2013) · cites this paper as Moskalenko et al, 2002
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- Authors
- Moskalenko, S., Henry, D. O., Rosse, C., Mirey, G., Camonis, J. H., & White, M. A.
- Affiliations
- The University of Texas Southwestern Medical Center (US)
- The University of Texas at Dallas (US)
- Inserm (FR)
- Corresponding author
- White, Michael A.
- Venue
- Nature Cell Biology
- Published
- 2001-12-10
- Volume
- 4
- Issue
- 1
- Pages
- 66-72
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W1970615437
- Cited by
- 423
- References
- 0
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 4 · 2023: 5 · 2022: 5 · 2021: 12 · 2020: 9 · 2019: 15 · 2018: 10 · 2017: 21 · 2016: 13 · 2015: 22 · 2014: 20 · 2013: 23 · 2012: 26
- FWCI
- 8.46
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- University of Texas Southwestern Medical Center
- National Cancer Institute (CA 71443, R01CA071443)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- exocyst, Ral GTPase, Sec5, vesicle trafficking, cell polarity, regulated exocytosis, epithelial cells, neuroendocrine cells
- MeSH terms
- Animals, Carrier Proteins, Exocytosis, Fungal Proteins, HeLa Cells, Humans, Membrane Proteins, Phospholipase D, Signal Transduction, PC12 Cells, Cell Polarity, Caco-2 Cells, GTPase-Activating Proteins, ral GTP-Binding Proteins, Protein Transport, Secretory Vesicles, Vesicular Transport Proteins, Rats
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