The DOI matches its Crossref record; 55 of the 55 references it cites were found in a registry or an index.
Expression system for structural and functional studies of human glycosylation enzymes
Open accessAbstract
Vertebrate glycoproteins and glycolipids are synthesized in complex biosynthetic pathways localized predominantly within membrane compartments of the secretory pathway. The enzymes that catalyze these reactions are exquisitely specific, yet few have been extensively characterized due to challenges associated with their recombinant expression as functional products. We used a modular approach to create an expression vector library encoding all known human glycosyltransferases, glycoside hydrolases, sulfotransferases, and other glycan modifying enzymes. We then expressed the enzymes as secreted catalytic domain fusion proteins in mammalian and insect cell hosts, purified and characterized a subset of the enzymes, and determined the structure of one, the sialyltransferase ST6GALNAC2. Many enzymes were produced at high yields and similar levels in both hosts, but individual protein expression levels varied widely. This expression vector library will be a transformative resource for recombinant enzyme production, broadly enabling structure-function studies and expanding applications of these enzymes in glycochemistry and glycobiology.
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Polymerization of the backbone of the pectic polysaccharide rhamnogalacturonan I
Amos, R. A., Atmodjo, M. A., Huang, C., Gao, Z., Venkat, A., Taujale, R., Kannan, N., Moremen, K. W., & Mohnen, D. (2022) · Nature Plants · doi:10.1038/s41477-022-01270-3 · Cited by 55
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Metadata
- Authors
- Moremen, K. W., Ramiah, A., Stuart, M., Steel, J., Meng, L., Forouhar, F., Moniz, H. A., Gahlay, G., Gao, Z., Chapla, D., Wang, S., Yang, J.-Y., Prabhakar, P. K., Johnson, R., Rosa, M. D., Geisler, C., Nairn, A. V., Seetharaman, J., Wu, S.-C., Tong, L., Gilbert, H. J., LaBaer, J., & Jarvis, D. L.
- Venue
- Nature Chemical Biology
- Published
- 2017-12-18
- Volume
- 14
- Issue
- 2
- Pages
- 156-162
- ISSN
- 1552-4450 (ISSN-L)
- 1552-4469
- OpenAlex
- W2777399253
- Cited by
- 284
- References
- 55
- FWCI
- 7.67
- Type
- journal article
- Language
- en
- Open access
- green
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- Moremen, K. W., Ramiah, A., Stuart, M., Steel, J., Meng, L., Forouhar, F., Moniz, H. A., Gahlay, G., Gao, Z., Chapla, D., Wang, S., Yang, J.-Y., Prabhakar, P. K., Johnson, R., Rosa, M. D., Geisler, C., Nairn, A. V., Seetharaman, J., Wu, S.-C., . . . Jarvis, D. L. (2018). Expression system for structural and functional studies of human glycosylation enzymes. Nature Chemical Biology. https://doi.org/10.1038/nchembio.2539
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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