The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
An engineered eukaryotic protein glycosylation pathway in Escherichia coli
Open accessAbstract
We performed bottom-up engineering of a synthetic pathway in E. coli for the production of eukaryotic trimannosyl chitobiose glycans and the transfer of these glycans to specific asparagine residues in target proteins. Glycan biosynthesis was enabled by four eukaryotic glycosyltransferases, including the yeast uridine diphosphate-N-acetylglucosamine transferases Alg13 and Alg14 and the mannosyltransferases Alg1 and Alg2. By including the bacterial oligosaccharyltransferase PglB from C. jejuni, glycans were successfully transferred to eukaryotic proteins.
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Cites
Intracellular Functions of N-Linked Glycans
Helenius, A., & Aebi, A. M. (2001) · Science · doi:10.1126/science.291.5512.2364 · Cited by 2,405
Cited by
Carbohydrate-active enzymes (CAZymes) in the gut microbiome
Wardman, J. F., Bains, R. K., Rahfeld, P., & Withers, S. G. (2022) · Nature Reviews Microbiology · doi:10.1038/s41579-022-00712-1 · Cited by 597
Production of Biopharmaceuticals in E. coli: Current Scenario and Future Perspectives
Baeshen, M. N., Al-Hejin, A. M., Bora, R. S., Ahmed, M. M. M., Ramadan, H. A. I., Saini, K. S., Baeshen, N. A., & Redwan, E. M. (2015) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.1412.12079 · Cited by 330
Protein Expression and Purification
Růčková, E., Müller, P., & Vojtěšek, B. (2014) · Klinicka onkologie · doi:10.14735/amko20141s92 · Cited by 328
Bacterial Protein N-Glycosylation: New Perspectives and Applications
Nothaft, H., & Szymanski, C. M. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.r112.417857 · Cited by 165
Expanding the glycoengineering toolbox: the rise of bacterial N-linked protein glycosylation
Baker, J. L., Çelik, E., & DeLisa, M. P. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2013.03.003 · Cited by 67
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Metadata
- Authors
- Valderrama-Rincon, J. D., Fisher, A. C., Merritt, J. H., Fan, Y.-Y., Reading, C. A., Chhiba, K., Heiss, C., Azadi, P., Aebi, M., & DeLisa, M. P.
- Venue
- Nature Chemical Biology
- Published
- 2012-03-25
- Volume
- 8
- Issue
- 5
- Pages
- 434-436
- ISSN
- 1552-4450 (ISSN-L)
- 1552-4469
- OpenAlex
- W2052626482
- Cited by
- 265
- References
- 1
- FWCI
- 9.29
- Type
- journal article
- Language
- en
- Open access
- green
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- Valderrama-Rincon, J. D., Fisher, A. C., Merritt, J. H., Fan, Y.-Y., Reading, C. A., Chhiba, K., Heiss, C., Azadi, P., Aebi, M., & DeLisa, M. P. (2012). An engineered eukaryotic protein glycosylation pathway in Escherichia coli. Nature Chemical Biology. https://doi.org/10.1038/nchembio.921
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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