The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Novel base-pairing interactions at the tRNA wobble position crucial for accurate reading of the genetic code
Open accessAbstract
Posttranscriptional modifications at the wobble position of transfer RNAs play a substantial role in deciphering the degenerate genetic code on the ribosome. The number and variety of modifications suggest different mechanisms of action during messenger RNA decoding, of which only a few were described so far. Here, on the basis of several 70S ribosome complex X-ray structures, we demonstrate how Escherichia coli tRNA LysUUU with hypermodified 5-methylaminomethyl-2-thiouridine (mnm 5 s 2 U) at the wobble position discriminates between cognate codons AAA and AAG, and near-cognate stop codon UAA or isoleucine codon AUA, with which it forms pyrimidine–pyrimidine mismatches. We show that mnm 5 s 2 U forms an unusual pair with guanosine at the wobble position that expands general knowledge on the degeneracy of the genetic code and specifies a powerful role of tRNA modifications in translation. Our models consolidate the translational fidelity mechanism proposed previously where the steric complementarity and shape acceptance dominate the decoding mechanism.
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Linking Crystallographic Model and Data Quality
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Cited by
Rozov, A., Khusainov, I., El Omari, K., Duman, R., Mykhaylyk, V., Yusupov, M., Westhof, E., Wagner, A., & Yusupova, G. (2019) · Nature Communications · doi:10.1038/s41467-019-10409-4 · Cited by 179
Self-citationCoevolution Theory of the Genetic Code at Age Forty: Pathway to Translation and Synthetic Life
Wong, J., Ng, S.-K., Mat, W.-K., Hu, T., & Xue, H. (2016) · Life · doi:10.3390/life6010012 · Cited by 93
Leonarski, F., D'Ascenzo, L., & Auffinger, P. (2019) · RNA · doi:10.1261/rna.068437.118 · Cited by 39
Deflating the RNA Mg 2+ bubble: stereochemistry to the rescue!
Auffinger, P., Ennifar, E., & D'Ascenzo, L. (2021) · RNA · doi:10.1261/rna.076067.120 · Cited by 29
Rangadurai, A., Szymanski, E. S., Kimsey, I., Shi, H., & Al-Hashimi, H. M. (2020) · Journal of Biomolecular NMR · doi:10.1007/s10858-020-00337-7 · Cited by 24
Metadata
- Authors
- Rozov, A., Demeshkina, N., Khusainov, I., Westhof, E., Yusupov, M., & Yusupova, G.
- Venue
- Nature Communications
- Published
- 2016-01-21
- Volume
- 7
- Issue
- 1
- Pages
- 10457-10457
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2302307165
- Cited by
- 188
- References
- 5
- FWCI
- 10.68
- Type
- journal article
- Language
- en
- Open access
- gold
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