The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Two-colour live-cell nanoscale imaging of intracellular targets
Open accessAbstract
Stimulated emission depletion (STED) nanoscopy allows observations of subcellular dynamics at the nanoscale. Applications have, however, been severely limited by the lack of a versatile STED-compatible two-colour labelling strategy for intracellular targets in living cells. Here we demonstrate a universal labelling method based on the organic, membrane-permeable dyes SiR and ATTO590 as Halo and SNAP substrates. SiR and ATTO590 constitute the first suitable dye pair for two-colour STED imaging in living cells below 50 nm resolution. We show applications with mitochondria, endoplasmic reticulum, plasma membrane and Golgi-localized proteins, and demonstrate continuous acquisition for up to 3 min at 2-s time resolution.
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Cites
Two-photon excitation STED microscopy in two colors in acute brain slices
Bethge, P., Chéreau, R., Avignone, E., Marsicano, G., & Nägerl, U. V. (2013) · Biophysical Journal · doi:10.1016/j.bpj.2012.12.054 · Cited by 139
Cited by
Fluorescence nanoscopy in cell biology
Sahl, S. J., Hell, S. W., & Jakobs, S. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.71 · Cited by 990
Endoplasmic reticulum-mitochondrial contactology: structure and signaling functions
Csordás, G., Weaver, D., & Hajnóczky, G. (2018) · Trends in Cell Biology · doi:10.1016/j.tcb.2018.02.009 · Cited by 625
Stimulated Emission Depletion Microscopy
Blom, H., & Widengren, J. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00653 · Cited by 339
Schmidt, R., Weihs, T., Wurm, C. A., Jansen, I., Rehman, J., Sahl, S. J., & Hell, S. W. (2021) · Nature Communications · doi:10.1038/s41467-021-21652-z · Cited by 306
Present Application and Perspectives of Organoid Imaging Technology
Fei, K., Zhang, J., Yuan, J., & Xiao, P. (2022) · Bioengineering · doi:10.3390/bioengineering9030121 · Cited by 65
Metadata
- Authors
- Bottanelli, F., Kromann, E. B., Allgeyer, E. S., Erdmann, R. S., Wood Baguley, S., Sirinakis, G., Schepartz, A., Baddeley, D., Toomre, D. K., Rothman, J. E., & Bewersdorf, J.
- Venue
- Nature Communications
- Published
- 2016-03-04
- Volume
- 7
- Issue
- 1
- Pages
- 10778-10778
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2334215271
- Cited by
- 221
- References
- 1
- FWCI
- 14.73
- Type
- journal article
- Language
- en
- Open access
- gold
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- Bottanelli, F., Kromann, E. B., Allgeyer, E. S., Erdmann, R. S., Wood Baguley, S., Sirinakis, G., Schepartz, A., Baddeley, D., Toomre, D. K., Rothman, J. E., & Bewersdorf, J. (2016). Two-colour live-cell nanoscale imaging of intracellular targets. Nature Communications. https://doi.org/10.1038/ncomms10778
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