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Cited by 164Genomics and Chromatin DynamicsRNA Research and SplicingNuclear Structure and Function

Cajal bodies are linked to genome conformation

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Wang, Qiuyan, Sawyer, Iain A., Sung, Myong-Hee, Sturgill, David, Shevtsov, Sergey P., Pegoraro, Gianluca, Hakim, Ofir, Baek, Songjoon +2 more·2016·Nature Communications
DOI 10.1038/ncomms10966PMC PMC4802181
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Abstract

The mechanisms underlying nuclear body (NB) formation and their contribution to genome function are unknown. Here we examined the non-random positioning of Cajal bodies (CBs), major NBs involved in spliceosomal snRNP assembly and their role in genome organization. CBs are predominantly located at the periphery of chromosome territories at a multi-chromosome interface. Genome-wide chromosome conformation capture analysis (4C-seq) using CB-interacting loci revealed that CB-associated regions are enriched with highly expressed histone genes and U small nuclear or nucleolar RNA (sn/snoRNA) loci that form intra- and inter-chromosomal clusters. In particular, we observed a number of CB-dependent gene-positioning events on chromosome 1. RNAi-mediated disassembly of CBs disrupts the CB-targeting gene clusters and suppresses the expression of U sn/snoRNA and histone genes. This loss of spliceosomal snRNP production results in increased splicing noise, even in CB-distal regions. Therefore, we conclude that CBs contribute to genome organization with global effects on gene expression and RNA splicing fidelity.

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  • A similar network of contacts is detectable between active promoters of multiple histone genes in many mammalian cell types.

    “A similar network of contacts is also detectable between the active promoters of multiple histone genes in many mammalian cell types [243, 279, 471, 492].”

    From Transcriptional enhancers and their communication with gene promoters (2021) · cites this paper as [492]

    The Results section, “Many inter-chromosomal interactions of HIST1 are CB proximal” (page not available), reports contacts involving histone gene clusters on chromosomes 1 and 6 in HeLa cells. It does not establish a network specifically between active histone-gene promoters or show that such contacts occur in many mammalian cell types. (LLM, conf=0.86)

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Cited by

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Authors
Wang, Q., Sawyer, I. A., Sung, M.-H., Sturgill, D., Shevtsov, S. P., Pegoraro, G., Hakim, O., Baek, S., Hager, G. L., & Dundr, M.
Affiliations
  • National Institutes of Health (US)
  • Rosalind Franklin University of Medicine and Science (US)
Corresponding author
  • Hager, Gordon L.
  • Dundr, Miroslav
ORCID
Venue
Nature Communications
Publisher
Springer Science and Business Media LLC
Published
2016-03-21
Volume
7
Issue
1
Pages
10966-10966
ISSN
2041-1723 (ISSN-L)
DOI
10.1038/ncomms10966
PMC
PMC4802181
OpenAlex
W2309374135
Cited by
164
References
5
Citations by year
2026: 4 · 2025: 11 · 2024: 17 · 2023: 16 · 2022: 16 · 2021: 16 · 2020: 26 · 2019: 13 · 2018: 17 · 2017: 14 · 2016: 13 · 2015: 1
FWCI
9.88
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01GM090156)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
Cajal bodies, nuclear bodies, chromosome conformation, chromosome territories, snRNP assembly, histone genes, gene localization, splicing fidelity, chromosome 1, 4C-seq
MeSH terms
Chromosomes, Human, HeLa Cells, Histones, Humans, Nucleic Acid Conformation, RNA Polymerase II, RNA Splicing, RNA, Small Nuclear, Transcription, Genetic, Reproducibility of Results, Genome, Human, Spliceosomes, Sequence Deletion, In Situ Hybridization, Fluorescence, RNA, Small Nucleolar, Coiled Bodies, Epigenesis, Genetic, Genetic Loci

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