A transgenic approach for controlling Lygus in cotton
Open accessAbstract
Lygus species of plant-feeding insects have emerged as economically important pests of cotton in the United States. These species are not controlled by commercial Bacillus thuringiensis (Bt) cotton varieties resulting in economic losses and increased application of insecticide. Previously, a Bt crystal protein (Cry51Aa2) was reported with insecticidal activity against Lygus spp. However, transgenic cotton plants expressing this protein did not exhibit effective protection from Lygus feeding damage. Here we employ various optimization strategies, informed in part by protein crystallography and modelling, to identify limited amino-acid substitutions in Cry51Aa2 that increase insecticidal activity towards Lygus spp. by >200-fold. Transgenic cotton expressing the variant protein, Cry51Aa2.834_16, reduce populations of Lygus spp. up to 30-fold in whole-plant caged field trials. One transgenic event, designated MON88702, has been selected for further development of cotton varieties that could potentially reduce or eliminate insecticide application for control of Lygus and the associated environmental impacts.
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Modified Cry51Aa2 protein (Cry51Aa2.834_16) protects cotton against feeding damage caused by hemipteran and thysanopteran pests.
“An example is modified Cry51Aa2 protein (Cry51Aa2.834_16)that protects cotton against feeding damage caused by hemipteran and thysanopteran pests (Baum et al., 2012;Gowda et al., 2016;Bachman et al., 2017).”
From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Gowda et al., 2016
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References 32 · Cited by 91
Research works printed but not linked: 3
- McPherson A. The Preparation and Analysis of Protein Crystals 94– 97John Wiley & Sons (1982).
- Levene H. Contributions to Probability and Statistics 278– 292Stanford Univ. Press (1960).
- Finney D. J. Probit Analysis 3rd ed Cambridge Univ. Press (1971).
- Authors
- Gowda, A., Rydel, T. J., Wollacott, A. M., Brown, R. S., Akbar, W., Clark, T. L., Flasinski, S., Nageotte, J. R., Read, A. C., Shi, X., Werner, B. J., Pleau, M. J., & Baum, J. A.
- Affiliations
- Monsanto (United States) (US)
- Corresponding author
- Gowda, Anilkumar
- Venue
- Nature Communications
- Published
- 2016-07-18
- Volume
- 7
- Issue
- 1
- Pages
- 12213-12213
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC4960306
- OpenAlex
- W2492586525
- Cited by
- 91
- References
- 32
- Citations by year
- 2026: 9 · 2025: 4 · 2024: 10 · 2023: 8 · 2022: 13 · 2021: 10 · 2020: 6 · 2019: 13 · 2018: 9 · 2017: 7 · 2016: 2
- FWCI
- 5.37
- Type
- journal article
- Language
- en
- Update notices
- correction (2020-02-26)
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) (submittedVersion, cc-by)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
- Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
- Keywords
- Lygus, cotton pests, transgenic cotton, Bacillus thuringiensis, amino acid substitutions, protein crystallography, insecticide reduction, phytophagous insects
- MeSH terms
- Bacillus thuringiensis Toxins, Animals, Bacterial Proteins, Biological Assay, Gossypium, Endotoxins, Hemolysin Proteins, Pest Control, Biological, Heteroptera, Plants, Genetically Modified, Mutant Proteins
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