BLISS is a versatile and quantitative method for genome-wide profiling of DNA double-strand breaks
Yan, Winston X., Mirzazadeh, Reza, Garnerone, Silvano, Scott, David, Schneider, Martin W., Kallas, Tomasz, Custodio, Joaquin, Wernersson, Erik, Li, Yinqing, Gao, Linyi, Federova, Yana, Zetsche, Bernd, Zhang, Feng, Bienko, Magda, Crosetto, Nicola·2017·Nature Communications
Cited by (369)
Items 301–369 of 369
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- Steroid‐Modulated Transcription Synergistically Forms DNA Double‐Strand Breaks With Topoisomerase II InhibitorYing Zhao, Tetsuro Hisayoshi, Doudou Zhang, Saaya Suzuki, Takashi Watanabe, Atsuo Kobayashi, Qianqian Guo, Yukihide Momozawa, Takashi Shimokawa, Shunsuke Kato et al. · 2025 · Cancer Science
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- Single-cell and in vivo profiling reveal heterogeneous and organ-specific CRISPR-Cas9 off-target and translocation outcomesAlexandra Madsen, Niklas Selfjord, Marta Martínez-Lage, Anna-Lena Loyd, Gavin Kurgan, Mikaela Stahlberg, Julia Lindgren, Julia Liz Touza, Leif Wigge, Mike A. Firth et al. · 2026 · Nature Communications
- Next Generation Sequence-based Technologies for Analyzing DNA Strand BreaksYui Fujiwara, Sachihiro Matsunaga, Takuya Sakamoto · 2021 · CYTOLOGIA
- Let’s not take DNA breaks for granted. The importance of direct detection of DNA breaks for the successful development of DDR inhibitorsKamil J. Solarczyk, Magdalena Kordon-Kiszala · 2023 · Frontiers in Cell and Developmental Biology
- ChromInSight: Revealing DNA Double‐Strand Breaks Through Chromatin Structural Insights With an Interpretable Graph Neural Network FrameworkKang Xu, YU Zong-yuan, Canzhuang Sun, Conglin Gou, Jiangyue Zhu, Jun Wang, Xiaochen Bo, Guoxian Yu, Hao Li, Hebing Chen · 2025 · Advanced Science
- Streptococcus uberis Cas9—A Compact Type II-A Nuclease Recognizing a Unique PAM and Functional in Human CellsA. Vasileva, Marina Abramova, P. Selkova, A. Arseniev, O. Musharova, Polina Malysheva, A. Demkina, M. Khodorkovskii, K. Severinov · 2025 · The CRISPR Journal
- CRISPR-based Technologies for Genome Engineering: Properties, Current Improvements and Applications in MedicineSylvain Geny, Elaheh Sadat Hosseini, Jean‐Paul Concordet, Carine Giovannangeli · 2019 · Royal Society of Chemistry eBooks
- Analyzing Homologous Recombination at a Genome-Wide LevelColine Arnould, Vincent Rocher, Gaëlle Legube · 2020 · Methods in molecular biology
- Chimeric crRNAs Retaining Activity of Cas12a with Potential to Improve SpecificityJihyun Park, Jaewoo Choi, Men Thi Hoai Duong, Hee‐Chul Ahn, Seung Woo Hong, Gil Tae Hwang, Jinsu An, Hak Suk Chung, Dae‐Ro Ahn · 2020 · Bulletin of the Korean Chemical Society
- Assessing for off-target mutagenesisKiran Musunuru · 2021 · Elsevier eBooks
- ERCC1-XPF Interacts with Topoisomerase IIβ to Facilitate the Repair of Activity-induced DNA BreaksGeorgia Chatzinikolaou, Kalliopi Stratigi, Kyriacos Agathangelou, Maria Tsekrekou, Evi Goulielmaki, Ourania Chatzidoukaki, Katerina Gkirtzimanaki, Tamara Aid‐Pavlidis, Michalis Aivaliotis, Pavlos Pavlidis et al. · 2020 · bioRxiv (Cold Spring Harbor Laboratory)
- From DNA Topology to Pubertal Onset: Using Integrative Genomic Approaches to Study Transcriptional RegulationHuayun Hou · 2019 · TSpace (University of Toronto)
- Engineered PAM-flexible FnCas9 variants for robust and specific genome editing and diagnosticsSundaram Acharya, Asgar Hussain Ansari, Seiichi Hirano, Deepanjan Paul, Riya Rauthan, Manoj Kumar, Rhythm Phutela, Sajal Sarkar, Sneha Gulati, Sudipta Mahato et al. · 2021 · Research Square
- Massively parallel genomic perturbations with multi-target CRISPR reveal new insights on Cas9 activity and DNA damage responses at endogenous sitesRoger S. Zou, Alberto Marín-González, Yang Liu, Hans B. Liu, Leo L. Shen, Rachel K. Dveirin, Jay X. J. Luo, Reza Kalhor, Taekjip Ha · 2022 · bioRxiv (Cold Spring Harbor Laboratory)
- Optimization of NLS Composition Improves CRISPR-Cas12a Editing Rates in Human Primary CellsKevin Luk, Pengpeng Liu, Jing Zeng, Yetao Wang, Stacy A. Maitland, Feston Idrizi, Karthikeyan Ponnienselvan, Lihua Julie Zhu, Jeremy Luban, Daniel E. Bauer et al. · 2022 · bioRxiv (Cold Spring Harbor Laboratory)
- Specificity of CRISPR‐Cas9 Gene EditingNiklas Selfjord, Alexandra Madsen, Pınar Akçakaya · 2022 · Wiley eBooks
- Target-matched increased fidelity SpCas9 variants can edit any target without genome-wide off-target effectsPéter István Kulcsár, András Tálas, Zoltán Ligeti, Eszter Tóth, Zsófia Rakvács, Vanessza Laura Végi, Sarah Laura Krausz, Krisztina Huszár, Ervin Welker · 2022 · bioRxiv (Cold Spring Harbor Laboratory)
- Detection and Quantification of Genome Editing Events in Preclinical and Clinical StudiesMarina Falaleeva, Shengdar Q. Tsai, Kathleen E. Meyer, Yanmei Lu · 2024 · Wiley eBooks
- Joint single-cell capture of Cas9 edits and transcriptomes reveals on- and off-target effects on gene expressionMichael H. Lorenzini, Brad Balderson, Shanna N. Lavalle, Karthyayani Sajeev, Aaron J. Ho, Madelyn Light, Olalekan H. Usman, Gavin Kurgan, Graham McVicker · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
- qDSB-Seq: quantitative DNA double-strand break sequencingYingjie Zhu, Anna Biernacka, Benjamin Pardo, Norbert Dojer, Romain Forey, Magdalena Skrzypczak, Bernard Fongang, Jules Nde, Raziyeh Yousefi, Philippe Pasero et al. · 2017 · bioRxiv (Cold Spring Harbor Laboratory)
- Generating Isogenic Models of Li-Fraumeni Syndrome and Restoring p53 using CRISPRJames Tran · 2020 · TSpace (University of Toronto)
- 農用トラクタの自動作業機能に関する研究 : IV. 油圧リンクによる播種深さ制御システムの開発實 小松, 輝明 佐藤 · 1995 · Institutional Repositories DataBase (IRDB)
- Replication origins drive genetic and phenotypic variation in humansPierre Murat, Guillaume Guilbaud, Julian E. Sale · 2021 · bioRxiv (Cold Spring Harbor Laboratory)
- Faculty Opinions recommendation of GPSeq reveals the radial organization of chromatin in the cell nucleus.Dana Branzei · 2021 · Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature
- Crispr-Embedding: CRISPR/Cas9 Off-Target Activity Prediction Using DNA k-Mer EmbeddingSwakkhar Shatabda, Anika Tahsin, Zarin Tasnim, Muneera Chowdhury, Kangkhita Hassin, Galib Hossain Meraz, Kazi Farzana Aziz · 2022 · SSRN Electronic Journal
- Molecular Mechanisms of Type II CRISPR ‐ Cas SystemsTautvydas Karvelis, Virginijus Šikšnys · 2022 · Wiley eBooks
- Nascent DNA sequencing and its diverse applications in genome integrity researchJacob Paiano, André Nussenzweig · 2023 · Methods in cell biology
- Data from AXL and Error-Prone DNA Replication Confer Drug Resistance and Offer Strategies to Treat EGFR-Mutant Lung CancerAshish Noronha, Nishanth Belugali Nataraj, Joo Sang Lee, Benny Zhitomirsky, Yaara Oren, Sara K. Oster, Moshit Lindzen, Saptaparna Mukherjee, Rainer D. Will, Soma Ghosh et al. · 2023
- Data from AXL and Error-Prone DNA Replication Confer Drug Resistance and Offer Strategies to Treat EGFR-Mutant Lung CancerAshish Noronha, Nishanth Belugali Nataraj, Joo Sang Lee, Benny Zhitomirsky, Yaara Oren, Sara K. Oster, Moshit Lindzen, Saptaparna Mukherjee, Rainer D. Will, Soma Ghosh et al. · 2023
- Sensitivity of DNA double-strand break loci is coupled with spatial interaction density of chromatinHebing Chen, Kang Xu, Bijia Chen, Qiu Xie, Yu Sun, Xiang Xu, Junting Wang, Yaru Li, Pengzhen Hu, Shutong Yue et al. · 2023 · Research Square
- Bioinformatics tools and databases in genome editing for plantsAiman Arif, Rana Ali Haider, Ahmad Munir, Muhammad Zubair Ghouri, Sultan Habibullah Khan, Kamel Ahmed Abd-Elsalam, Aftab Ahmad · 2024 · Elsevier eBooks
- Additional file 2 of A graph neural network-based interpretable framework reveals a novel DNA fragility–associated chromatin structural unitYu Sun, Xiang Xu, Lin, Lin, Xu, Kang, Yang Zheng, Ren, Chao, Huan Tao, Xu Wang, Huan Zhao, Wei-Wei Tu et al. · 2023 · Figshare
- Resveratrol targets G-quadruplexes to exert its pharmacological effectsAna Soriano‐Lerma, Victoria Sánchez-Martín, Javier Murciano‐Calles, Matilde Ortiz-González, Maria J. Tello-Lopez, Virginia Pérez‐Carrasco, Ángel Linde-Rodríguez, Inmaculada Ramírez-Macías, Irene Gómez‐Pinto, Inmaculada López‐Aliaga et al. · 2024 · bioRxiv (Cold Spring Harbor Laboratory)
- DNA Damage, Repair, and Advanced DNA Damage Detection TechnologiesArlet Hernandez, Kaveri Goel, Saddam Hussain, Natalie R. Gassman · 2024 · Elsevier eBooks
- Precise Mapping of Physiological DSBs Using In-Suspension Break Labeling In Situ and Sequencing (sBLISS)Osama Hidmi, Sara K. Oster, Diala Shatleh, Jonathan Monin, Rami I. Aqeilan · 2025 · Methods in molecular biology
- Development of new increased fidelity SpCas9 variantsPéter István Kulcsár · 2019
- Fast and accurate quantification of double-strand breaks in microsatellites by digital PCRCécile Palao, Adèle Kovacs, Maria Teresa Teixeira, Guy‐Franck Richard · 2025 · Biology Methods and Protocols
- The multiscale distribution of radiation-induced DNA damage and its impact on local genome structureHeng Li, Tianchun Xue, Rachel Patton McCord · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
- Integrated In Vivo, In Vitro, and In Silico Evaluation of Spermacoce ocymoides Methanolic Extract for Antidiarrheal, Thrombolytic, and Antiarthritic ActivitiesBakul Akter, Kutub Uddin Ahamed, Md. Hossain Sohrab, Silvia Aishee, Bidhan Sarkar, Afra Anika, Mohammed Kamrul Hossain, Hanan M. Alharbi, Emad Rashad Sindi, Most. Nazmin Aktar · 2025 · Cell Biochemistry and Biophysics
- Sensitive, direct detection of non-coding off-target base editor unwinding and editing in primary cellsTong Wang, Selin Jessa, Georgi K. Marinov, Sandy L. Klemm, Anshul Bharat Kundaje, William James Greenleaf · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
- Genome Editing as Genetic Engineering Tool: Potential Applications in Production of Microbial Foods for Food SecurityOluwafemi S. Obayori, Oluwaseun Oyetunji Olasunkanmi · 2025 · Springer eBooks
- A novel class of non-coding variants driving DNA double-strand breaks is associated with complex genetic diseasesSébastien Auber, Sarah Collins, Andrey Buyan, Ariuna Z Aiusheeva, Anne-Laure Finoux, Florian Saur, Sarah Cohen, Vincent Rocher, Coline Arnould, Marie Verbanck et al. · 2025 · medRxiv
- Additional file 4 of Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approachesJeonghun Kwon, Kim, Minyoung, Hwang, Woochang, Anna Jo, Hwang, Gue-Ho, Jung, Minhee, Kim, Un Gi, Gang Cui, Heon Seok Kim, Eom, Joon-Ho et al. · 2023 · Figshare
- Additional file 2 of ITR-Seq, a next-generation sequencing assay, identifies genome-wide DNA editing sites in vivo following adeno-associated viral vector-mediated genome editingCamilo Ayala Breton, Peter Clark, Wang, Lili, Jenny A. Greig, James M. Wilson · 2020 · Figshare
- Additional file 3 of A graph neural network-based interpretable framework reveals a novel DNA fragility–associated chromatin structural unitSun, Yu, Xu, Xiang, Lin Lin, Xu, Kang, Zheng, Yang, Ren, Chao, Huan Tao, Wang, Xu, Huan Zhao, Tu, Weiwei et al. · 2023 · Figshare
- Additional file 1 of ITR-Seq, a next-generation sequencing assay, identifies genome-wide DNA editing sites in vivo following adeno-associated viral vector-mediated genome editingCamilo Ayala Breton, Clark, Peter M., Wang, Lili, Jenny A. Greig, James M. Wilson · 2020 · Figshare
- Additional file 3 of ITR-Seq, a next-generation sequencing assay, identifies genome-wide DNA editing sites in vivo following adeno-associated viral vector-mediated genome editingCamilo Ayala Breton, Peter Clark, Wang, Lili, Jenny A. Greig, James M. Wilson · 2020 · Figshare
- Additional file 2 of CasKAS: direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mappingGeorgi K. Marinov, Samuel H. Kim, S. Tansu Bagdatli, Soon Il Higashino, Alexandro E. Trevino, Josh Tycko, Tong Wu, Lacramioara Bintu, Michael Cory Bassik, Chuan He et al. · 2023 · Figshare
- Additional file 1 of CasKAS: direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA mappingGeorgi K. Marinov, Samuel H. Kim, S. Tansu Bagdatli, Soon Il Higashino, Alexandro E. Trevino, Josh Tycko, Tong Wu, Lacramioara Bintu, Michael Cory Bassik, Chuan He et al. · 2023 · Figshare
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- Additional file 1 of A graph neural network-based interpretable framework reveals a novel DNA fragility–associated chromatin structural unitSun, Yu, Xu, Xiang, Lin Lin, Xu, Kang, Zheng, Yang, Ren, Chao, Huan Tao, Wang, Xu, Huan Zhao, Tu, Weiwei et al. · 2023 · Figshare
- Additional file 6 of Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approachesJeonghun Kwon, Kim, Minyoung, Hwang, Woochang, Anna Jo, Hwang, Gue-Ho, Jung, Minhee, Kim, Un Gi, Gang Cui, Heon Seok Kim, Eom, Joon-Ho et al. · 2023 · Figshare
- Additional file 3 of Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approachesJeonghun Kwon, Kim, Minyoung, Woo Chang Hwang, Anna Jo, Hwang, Gue-Ho, Jung, Minhee, Kim, Un Gi, Gang Cui, Heon Seok Kim, Eom, Joon-Ho et al. · 2023 · Figshare
- Additional file 4 of A graph neural network-based interpretable framework reveals a novel DNA fragility–associated chromatin structural unitSun, Yu, Xu, Xiang, Lin, Lin, Xu, Kang, Zheng, Yang, Ren, Chao, Huan Tao, Wang, Xu, Huan Zhao, Tu, Weiwei et al. · 2023 · Figshare
- Decoding DNA metabolism and its clinical relevance through the lens of high-throughput sequencing assaysJingzhi Luo, Fanyu Zhao, Shuyan Lin, Yang Liu · 2026 · Medical Review
- 3-D Genome organization of DNA damage repairUjjwal Kumar Banerjee · 2017 · theses.fr (ABES)
- CRISPR-Cas-Based Editing Technologies in Preclinical Models for Disease CorrectionPrakriti Karki, Sudarshan GC · 2026 · Genetics.
- Landscape of plastid DNA breaks in Arabidopsis during development and environmental stimulusWenjie Wang, Chengxia Zheng, Kuan Li, Qianwen Sun · 2026 · The Plant Cell
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