Lansoprazole is an antituberculous prodrug targeting cytochrome bc1

Abstract

Better antibiotics capable of killing multi-drug-resistant Mycobacterium tuberculosis are urgently needed. Despite extensive drug discovery efforts, only a few promising candidates are on the horizon and alternative screening protocols are required. Here, by testing a panel of FDA-approved drugs in a host cell-based assay, we show that the blockbuster drug lansoprazole (Prevacid), a gastric proton-pump inhibitor, has intracellular activity against M. tuberculosis . Ex vivo pharmacokinetics and target identification studies reveal that lansoprazole kills M. tuberculosis by targeting its cytochrome bc 1 complex through intracellular sulfoxide reduction to lansoprazole sulfide. This novel class of cytochrome bc 1 inhibitors is highly active against drug-resistant clinical isolates and spares the human H + K + -ATPase thus providing excellent opportunities for targeting the major pathogen M. tuberculosis . Our finding provides proof of concept for hit expansion by metabolic activation, a powerful tool for antibiotic screens.