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Infection of human cells by an endogenous retrovirus of pigs

Patience, Clive, Takeuchi, Yasuhiro, Weiss, Robin A.·1997·Nature Medicine
DOI 10.1038/nm0397-282
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Abstract

The possible use of pig organs and tissues as xenografts in humans is actively being considered in biomedical research. We therefore examined whether pig endogenous retrovirus (PERV) genomes can be infectiously transmitted to human cells in culture. Two pig kidney cell lines spontaneously produce C-type retrovirus particles. Cell-free retrovirus produced by the PK-15 kidney cell line (PERV-PK) infected pig, mink and human kidney 293 cell lines and co-cultivation of X-irradiated PK-15 cells with human cells resulted in a broader range of human cell infection, including human diploid fibroblasts and B- and T-cell lines. Kidney, heart and spleen tissue obtained from domestic pigs contained multiple copies of integrated PERV genomes and expressed viral RNA. Upon passage in human cells PERV-PK could rescue a Moloney retroviral vector and acquired resistance to lysis by human complement.

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  • The donors carried porcine endogenous retrovirus (PERV) sequences in their genome, which present a zoonotic risk, as PERV transmission to human cells in culture and their integration into the human genome have been demonstrated.

    “Third, the donors carried porcine endogenous retrovirus (PERV) sequences in their genome, which present a zoonotic risk, as PERV transmission to human cells in culture and their integration into the human genome have been demonstrated11,12.”

    From Design and testing of a humanized porcine donor for xenotransplantation (2023) · cites this paper as [12]

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Metadata

Authors
Patience, C., Takeuchi, Y., & Weiss, R. A.
Venue
Nature Medicine
Publisher
Springer Science and Business Media LLC
Published
1997-03
Volume
3
Issue
3
Pages
282-286
ISSN
  • 1078-8956
  • 1546-170X
DOI
10.1038/nm0397-282
Cited by
953
References
1
Language
en
Licence
www.springer.com/tdm (tdm)

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