Induction of dopaminergic neuron phenotype in the midbrain by sonic hedgehog protein
Abstract
Loss of substantia nigra dopaminergic neurons, which develop from the ventral region of the midbrain, is associated with Parkinson's disease. During embryogenesis, induction of these and other ventral neurons is influenced by interactions with the induction of mesoderm of the notochord and the floor plate, which lies at the ventral midline of the developing CNS. Sonic hedgehog encodes a secreted peptide, which is expressed in notochord and floor plate cells and can induce appropriate ventral cell types in the basal forebrain and spinal cord. Here we demonstrate that Sonic hedgehog is sufficient to induce dopaminergic and other neuronal phenotypes in chick mesencephalic explants in vitro. We find that Sonic hedgehog is a general ventralizing signal in the CNS, the specific response being determined by the receiving cells. These results suggest that Sonic hedgehog may have utility in the induction of clinically important cell types.
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Sonic hedgehog protein is important in maturation of dopaminergic neurons.
“As sonic hedgehog protein is very important in the maturation of dopaminergic neurons (Wang et al., 1995)”
From Neuroinflammation as a risk factor for attention deficit hyperactivity disorder (2019) · cites this paper as Wang et al., 1995
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- Authors
- Wang, M. Z., Jin, P., Bumcrot, D. A., Marigo, V., Mcmahon, A. P., Wang, E. A., Woolf, T., & Pang, K.
- Affiliations
- Harvard University (US)
- Venue
- Nature Medicine
- Published
- 1995-11
- Volume
- 1
- Issue
- 11
- Pages
- 1184-1188
- ISSN
- 1078-8956 (ISSN-L)
- 1546-170X
- OpenAlex
- W2010410556
- Cited by
- 158
- References
- 29
- Citations by year
- 2024: 1 · 2023: 2 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 2 · 2016: 3 · 2015: 1 · 2014: 6 · 2013: 3 · 2012: 6
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Hedgehog Signaling Pathway Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- Sonic hedgehog, dopaminergic neurons, substantia nigra, midbrain, Parkinson's disease, neuronal differentiation, ventralization, floor plate, notochord, chick embryo, cell fate specification
- MeSH terms
- Animals, Base Sequence, Chick Embryo, Dihydroxyphenylalanine, Dopamine, Embryonic Induction, Enzyme Induction, Mesencephalon, Molecular Sequence Data, Neurons, Parkinson Disease, Proteins, Recombinant Proteins, Tyrosine 3-Monooxygenase, Biomarkers, Trans-Activators, Polymerase Chain Reaction, Culture Techniques, Mice, Hedgehog Proteins
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