The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Rational design and application of responsive α-helical peptide hydrogels
Open accessAbstract
Biocompatible hydrogels have a wide variety of potential applications in biotechnology and medicine, such as the controlled delivery and release of cells, cosmetics and drugs; and as supports for cell growth and tissue engineering1. Rational peptide design and engineering are emerging as promising new routes to such functional biomaterials2-4. Here we present the first examples of rationally designed and fully characterized self-assembling hydrogels based on standard linear peptides with purely α-helical structures, which we call hydrogelating self-assembling fibres (hSAFs). These form spanning networks of α-helical fibrils that interact to give self-supporting physical hydrogels of >99% water content. The peptide sequences can be engineered to alter the underlying mechanism of gelation and, consequently, the hydrogel properties. Interestingly, for example, those with hydrogen-bonded networks melt upon heating, whereas those formed via hydrophobic interactions strengthen when warmed. The hSAFs are dual-peptide systems that only gel on mixing, which gives tight control over assembly5. These properties raise possibilities for using the hSAFs as substrates in cell culture. We have tested this in comparison with the widely used Matrigel substrate, and demonstrate that, like Matrigel, hSAFs support both growth and differentiation of rat adrenal pheochromocytoma cells for sustained periods in culture.
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Cites
Self-Assembly and Mineralization of Peptide-Amphiphile Nanofibers
Hartgerink, J. D., Beniash, E., & Stupp, S. I. (2001) · Science · doi:10.1126/science.1063187 · Cited by 3,601
Debnath, J., Muthuswamy, S. K., & Brugge, J. S. (2003) · Methods · doi:10.1016/s1046-2023(03)00032-x · Cited by 1,995
Spontaneous assembly of a self-complementary oligopeptide to form a stable macroscopic membrane.
Zhang, S., Holmes, T., Lockshin, C., & Rich, A. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.8.3334 · Cited by 1,304
Hirst, A. R., Escuder, B., Miravet, J. F., & Smith, D. K. (2008) · Angewandte Chemie International Edition · doi:10.1002/anie.200800022 · Cited by 1,273
Designing peptide based nanomaterials
Ulijn, R. V., & Smith, A. M. (2008) · Chemical Society Reviews · doi:10.1039/b609047h · Cited by 1,132
Reversible Hydrogels from Self-Assembling Artificial Proteins
Petka, W. A., Harden, J. L., McGrath, K. P., Wirtz, D., & Tirrell, D. A. (1998) · Science · doi:10.1126/science.281.5375.389 · Cited by 1,076
Glutamine repeats as polar zippers: their possible role in inherited neurodegenerative diseases.
Perutz, M. F., Johnson, T., Suzuki, M., & Finch, J. T. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.12.5355 · Cited by 1,062
Responsive Hydrogels from the Intramolecular Folding and Self-Assembly of a Designed Peptide
Schneider, J. P., Pochan, D. J., Ozbas, B., Rajagopal, K., Pakstis, L., & Kretsinger, J. (2002) · Journal of the American Chemical Society · doi:10.1021/ja027993g · Cited by 947
Responsive gels formed by the spontaneous self-assembly of peptides into polymeric β-sheet tapes
Aggeli, A., Bell, M., Boden, N., Keen, J. N., Knowles, P. F., McLeish, T. C. B., Pitkeathly, M., & Radford, S. E. (1997) · Nature · doi:10.1038/386259a0 · Cited by 858
Hybrid hydrogels assembled from synthetic polymers and coiled-coil protein domains
Wang, C., Stewart, R. J., & KopeČek, J. (1999) · Nature · doi:10.1038/17092 · Cited by 583
Cited by
Wang, B., Yang, W., McKittrick, J., & Meyers, M. A. (2016) · Progress in Materials Science · doi:10.1016/j.pmatsci.2015.06.001 · Cited by 873
Molecular self-assembly and applications of designer peptide amphiphiles
Zhao, X., Pan, F., Xu, H., Yaseen, M., Shan, H., Hauser, C. A. E., Zhang, S., & Lu, J. R. (2010) · Chemical Society Reviews · doi:10.1039/b915923c · Cited by 668
Sterilizable Gels from Thermoresponsive Block Copolymer Worms
Blanazs, A., Verber, R., Mykhaylyk, O. O., Ryan, A. J., Heath, J. Z., Douglas, C. W. I., & Armes, S. P. (2012) · Journal of the American Chemical Society · doi:10.1021/ja3024059 · Cited by 396
Design and applications of man-made biomimetic fibrillar hydrogels
Prince, E., & Kumacheva, E. (2019) · Nature Reviews Materials · doi:10.1038/s41578-018-0077-9 · Cited by 379
Deng, C., Wu, J., Cheng, R., Meng, F., Klok, H.-A., & Zhong, Z. (2014) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2013.10.008 · Cited by 371
Metadata
- Authors
- Banwell, E. F., Abelardo, E. S., Adams, D. J., Birchall, M. A., Corrigan, A., Donald, A. M., Kirkland, M., Serpell, L. C., Butler, M. F., & Woolfson, D. N.
- Venue
- Nature Materials
- Published
- 2009-07
- Volume
- 8
- Issue
- 7
- Pages
- 596-600
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- OpenAlex
- W2132237810
- Cited by
- 478
- References
- 29
- FWCI
- 15.31
- Type
- journal article
- Language
- en
- Open access
- green
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- Banwell, E. F., Abelardo, E. S., Adams, D. J., Birchall, M. A., Corrigan, A., Donald, A. M., Kirkland, M., Serpell, L. C., Butler, M. F., & Woolfson, D. N. (2009). Rational design and application of responsive α-helical peptide hydrogels. Nature Materials. https://doi.org/10.1038/nmat2479
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