The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.
Differential basal-to-apical accessibility of lamin A/C epitopes in the nuclear lamina regulated by changes in cytoskeletal tension
Open accessAbstract
Nuclear lamins play central roles at the intersection between cytoplasmic signalling and nuclear events. Here, we show that at least two N- and C-terminal lamin epitopes are not accessible at the basal side of the nuclear envelope under environmental conditions known to upregulate cell contractility. The conformational epitope on the Ig-domain of A-type lamins is more buried in the basal than apical nuclear envelope of human mesenchymal stem cells undergoing osteogenesis (but not adipogenesis), and in fibroblasts adhering to rigid (but not soft) polyacrylamide hydrogels. This structural polarization of the lamina is promoted by compressive forces, emerges during cell spreading, and requires lamin A/C multimerization, intact nucleoskeleton-cytoskeleton linkages (LINC), and apical-actin stress-fibre assembly. Notably, the identified Ig-epitope overlaps with emerin, DNA and histone binding sites, and comprises various laminopathy mutation sites. Our findings should help decipher how the physical properties of cellular microenvironments regulate nuclear events.
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“To overcome this limitation, novel 3D cell culture platforms are being created to better mimic in vivo conditions and are sometimes called spheroid or organoid culture, as described below (37,61,84,98,117,118).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [61]
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“This induced polarity may alter the functions of native cells with regard to spreading, migrating, and sensing soluble factors and other microenvironmental cues (61).”
From Modeling physiological events in 2D vs. 3D cell culture (2017) · cites this paper as [61]
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Citations
Cites
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“The resist pattern was rendered biopassive by 1 h incubation (RT) with 100 μg/ml PLL-g[3.5]-PEG(2) (SuSoS AG) in PBS.”
InfluentialEffects of substrate stiffness on cell morphology, cytoskeletal structure, and adhesion
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Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions
Guelen, L., Pagie, L., Brasset, É., Meuleman, W. R. A., Faza, M. B., Talhout, W., Eussen, B. H. J., de Klein, A., Wessels, L. F. A., de Laat, W. L., et al. (2008) · Nature · doi:10.1038/nature06947 · Cited by 2,042
Nuclear Lamin-A Scales with Tissue Stiffness and Enhances Matrix-Directed Differentiation
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Modeling physiological events in 2D vs. 3D cell culture
Duval, K., Grover, H., Han, L.-H., Mou, Y., Pegoraro, A. F., Fredberg, J., & Chen, Z. (2017) · Physiology · doi:10.1152/physiol.00036.2016 · Cited by 1,849
Integrins as biomechanical sensors of the microenvironment
Kechagia, J. Z., Ivaska, J., & Roca-Cusachs, P. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0134-2 · Cited by 1,311
Emerging views of the nucleus as a cellular mechanosensor
Kirby, T. J., & Lammerding, J. (2018) · Nature Cell Biology · doi:10.1038/s41556-018-0038-y · Cited by 594
Mechanics and functional consequences of nuclear deformations
Kalukula, Y., Stephens, A. D., Lammerding, J., & Gabriele, S. (2022) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-022-00480-z · Cited by 475
Regulation of genome organization and gene expression by nuclear mechanotransduction
Uhler, C., & Shivashankar, G. V. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.101 · Cited by 460
Metadata
- Authors
- Ihalainen, T. O., Aires, L., Herzog, F. A., Schwartlander, R., Moeller, J., & Vogel, V.
- Affiliations
- Tampere University (FI)
- ETH Zurich (CH)
- Venue
- Nature Materials
- Published
- 2015-08-24
- Volume
- 14
- Issue
- 12
- Pages
- 1252-1261
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- PMC
- PMC4655446
- OpenAlex
- W1201251126
- Cited by
- 172
- References
- 52
- Citations by year
- 2026: 8 · 2025: 17 · 2024: 5 · 2023: 21 · 2022: 17 · 2021: 14 · 2020: 17 · 2019: 26 · 2018: 19 · 2017: 13 · 2016: 14
- FWCI
- 6.66
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Nature Materials (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (133122)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Keywords
- lamin A/C, nuclear lamina, nuclear envelope, epitope accessibility, cytoskeletal tension, LINC complex, stress fibers, cell contractility, mesenchymal stem cells, bone formation, fibroblasts, polyacrylamide hydrogel, laminopathies, emerin
- MeSH terms
- Biopolymers, Cytoskeleton, Humans, Protein Conformation, Nuclear Lamina, Lamin Type A
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