The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A genetically encoded fluorescent reporter of ATP:ADP ratio
Open accessAbstract
We constructed a fluorescent sensor of adenylate nucleotides by combining a circularly permuted variant of GFP with a bacterial regulatory protein, GlnK1, from Methanococcus jannaschii. The sensor's affinity for Mg-ATP was <100 nM, as seen for other members of the bacterial PII regulator family, a surprisingly high affinity given that normal intracellular ATP concentration is in the millimolar range. ADP bound the same site of the sensor as Mg-ATP, competing with it, but produced a smaller change in fluorescence. At physiological ATP and ADP concentrations, the binding site is saturated, but competition between the two substrates causes the sensor to behave as a nearly ideal reporter of the ATP:ADP concentration ratio. This principle for sensing the ratio of two analytes by competition at a high-affinity site probably underlies the normal functioning of PII regulatory proteins. The engineered sensor, Perceval, can be used to monitor the ATP:ADP ratio during live-cell imaging.
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Cites
AMP-Activated Protein Kinase: An Ultrasensitive System for Monitoring Cellular Energy Charge
HARDIE, D. G., SALT, I. P., HAWLEY, S. A., & DAVIES, S. P. (1999) · Biochemical Journal · doi:10.1042/bj3380717 · Cited by 362
Cited by
Islam, M. N., Das, S. R., Emin, M. T., Wei, M., Sun, L., Westphalen, K., Rowlands, D. J., Quadri, S. K., Bhattacharya, S., & Bhattacharya, J. (2012) · Nature Medicine · doi:10.1038/nm.2736 · Cited by 1,585
Selective neuronal vulnerability in Parkinson disease
Surmeier, D. J., Obeso, J. A., & Halliday, G. M. (2017) · Nature Reviews Neuroscience · doi:10.1038/nrn.2016.178 · Cited by 1,116
Activity-driven local ATP synthesis is required for synaptic function
Rangaraju, V., Calloway, N., & Ryan, T. A. (2014) · Cell · doi:10.1016/j.cell.2013.12.042 · Cited by 836
Akerboom, J., Carreras Calderón, N., Tian, L., Wabnig, S., Prigge, M., Tolö, J., Gordus, A., Orger, M. B., Severi, K. E., Macklin, J. J., et al. (2013) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2013.00002 · Cited by 719
Bacterial metabolite indole modulates incretin secretion from intestinal enteroendocrine L cells
Chimerel, C., Emery, E., Summers, D. K., Keyser, U., Gribble, F. M., & Reimann, F. (2014) · Cell Reports · doi:10.1016/j.celrep.2014.10.032 · Cited by 548
Metadata
- Authors
- Berg, J., Hung, Y. P., & Yellen, G.
- Venue
- Nature Methods
- Published
- 2009-01-04
- Volume
- 6
- Issue
- 2
- Pages
- 161-166
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2129558617
- Cited by
- 475
- References
- 1
- FWCI
- 12.75
- Type
- journal article
- Language
- en
- Open access
- green
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