The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms
Open accessAbstract
Biological pathways are structured in complex networks of interacting genes. Solving the architecture of such networks may provide valuable information, such as how microorganisms cause disease. Here we present a method (Tn-seq) for accurately determining quantitative genetic interactions on a genome-wide scale in microorganisms. Tn-seq is based on the assembly of a saturated Mariner transposon insertion library. After library selection, changes in frequency of each insertion mutant are determined by sequencing the flanking regions en masse. These changes are used to calculate each mutant's fitness. Using this approach, we determined fitness for each gene of Streptococcus pneumoniae, a causative agent of pneumonia and meningitis. A genome-wide screen for genetic interactions of five query genes identified both alleviating and aggravating interactions that could be divided into seven distinct categories. Owing to the wide activity of the Mariner transposon, Tn-seq has the potential to contribute to the exploration of complex pathways across many different species.
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Cytoscape: A software Environment for integrated models of biomolecular interaction networks
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Ultrafast and memory-efficient alignment of short DNA sequences to the human genome.
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Genes required for mycobacterial growth defined by high density mutagenesis
Sassetti, C. M., Boyd, D. H., & Rubin, E. J. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03425.x · Cited by 2,544
Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants
Tong, A. H. Y., Evangelista, M., Parsons, A. B., Xu, H., Bader, G. D., Pagé, N., Robinson, M., Raghibizadeh, S., Hogue, C. W. V., Bussey, H., et al. (2001) · Science · doi:10.1126/science.1065810 · Cited by 2,138
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Conservation and Rewiring of Functional Modules Revealed by an Epistasis Map in Fission Yeast
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Cited by
Griffin, J. E., Gawronski, J. D., DeJesus, M. A., Ioerger, T. R., Akerley, B. J., & Sassetti, C. M. (2011) · PLoS Pathogens · doi:10.1371/journal.ppat.1002251 · Cited by 1,048
DeJesus, M. A., Gerrick, E. R., Xu, W., Park, S. W., Long, J. E., Boutte, C. C., Rubin, E. J., Schnappinger, D., Ehrt, S., Fortune, S. M., et al. (2017) · mBio · doi:10.1128/mbio.02133-16 · Cited by 668
Mutant phenotypes for thousands of bacterial genes of unknown function
Price, M. N., Wetmore, K. M., Waters, R. J., Callaghan, M., Ray, J., Liu, H., Kuehl, J. V., Melnyk, R. A., Lamson, J. S., Suh, Y., et al. (2018) · Nature · doi:10.1038/s41586-018-0124-0 · Cited by 576
Transposon insertion sequencing: a new tool for systems-level analysis of microorganisms
van Opijnen, T., & Camilli, A. (2013) · Nature Reviews Microbiology · doi:10.1038/nrmicro3033 · Cited by 494
Self-citationAlsford, S., Turner, D. J., Obado, S. O., Sanchez-Flores, A., Glover, L., Berriman, M., Hertz-Fowler, C., & Horn, D. (2011) · Genome Research · doi:10.1101/gr.115089.110 · Cited by 470
Metadata
- Authors
- van Opijnen, T., Bodi, K. L., & Camilli, A.
- Venue
- Nature Methods
- Published
- 2009-09-20
- Volume
- 6
- Issue
- 10
- Pages
- 767-772
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2030924818
- Cited by
- 933
- References
- 7
- FWCI
- 13.19
- Type
- journal article
- Language
- en
- Open access
- green
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- van Opijnen, T., Bodi, K. L., & Camilli, A. (2009). Tn-seq: high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms. Nature Methods. https://doi.org/10.1038/nmeth.1377
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