The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Optimized localization analysis for single-molecule tracking and super-resolution microscopy
Open accessAbstract
We optimally localized isolated fluorescent beads and molecules imaged as diffraction-limited spots, determined the orientation of molecules and present reliable formulas for the precision of various localization methods. Both theory and experimental data showed that unweighted least-squares fitting of a Gaussian squanders one-third of the available information, a popular formula for its precision exaggerates beyond Fisher's information limit, and weighted least-squares may do worse, whereas maximum-likelihood fitting is practically optimal.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Precise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Cited by
Dynamic regulation of transcriptional states by chromatin and transcription factors
Voss, T. C., & Hager, G. L. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3623 · Cited by 504
Bright photoactivatable fluorophores for single-molecule imaging
Grimm, J. B., English, B. P., Choi, H., Muthusamy, A. K., Mehl, B. P., Dong, P., Brown, T. A., Lippincott-Schwartz, J., Liu, Z., Lionnet, T., et al. (2016) · Nature Methods · doi:10.1038/nmeth.4034 · Cited by 435
Quantitative evaluation of software packages for single-molecule localization microscopy
Sage, D., Kirshner, H., Pengo, T., Stuurman, N., Min, J., Manley, S., & Unser, M. (2015) · Nature Methods · doi:10.1038/nmeth.3442 · Cited by 396
Switchable Fluorophores for Single-Molecule Localization Microscopy
Li, H., & Vaughan, J. C. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00767 · Cited by 319
Kutuzov, N., Flyvbjerg, H., & Lauritzen, M. (2018) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1802155115 · Cited by 250
Self-citation
Metadata
- Authors
- Mortensen, K. I., Churchman, L. S., Spudich, J. A., & Flyvbjerg, H.
- Venue
- Nature Methods
- Published
- 2010-04-04
- Volume
- 7
- Issue
- 5
- Pages
- 377-381
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2070353646
- Cited by
- 871
- References
- 1
- FWCI
- 26.94
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Optimized localization analysis for single-molecule tracking and super-resolution microscopy. https://app.cochat.ai/papers/10.1038/nmeth.1447
- The paper
- Mortensen, K. I., Churchman, L. S., Spudich, J. A., & Flyvbjerg, H. (2010). Optimized localization analysis for single-molecule tracking and super-resolution microscopy. Nature Methods. https://doi.org/10.1038/nmeth.1447
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
