The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Measuring image resolution in optical nanoscopy
Open accessAbstract
Resolution in optical nanoscopy (or super-resolution microscopy) depends on the localization uncertainty and density of single fluorescent labels and on the sample's spatial structure. Currently there is no integral, practical resolution measure that accounts for all factors. We introduce a measure based on Fourier ring correlation (FRC) that can be computed directly from an image. We demonstrate its validity and benefits on two-dimensional (2D) and 3D localization microscopy images of tubulin and actin filaments. Our FRC resolution method makes it possible to compare achieved resolutions in images taken with different nanoscopy methods, to optimize and rank different emitter localization and labeling strategies, to define a stopping criterion for data acquisition, to describe image anisotropy and heterogeneity, and even to estimate the average number of localizations per emitter. Our findings challenge the current focus on obtaining the best localization precision, showing instead how the best image resolution can be achieved as fast as possible.
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Rosenthal, P. B., & Henderson, R. (2003) · Journal of Molecular Biology · doi:10.1016/j.jmb.2003.07.013 · Cited by 2,416
Veatch, S. L., Machta, B. B., Shelby, S. A., Chiang, E. N., Holowka, D. A., & Baird, B. A. (2012) · PLoS ONE · doi:10.1371/journal.pone.0031457 · Cited by 290
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Ludtmann, M. H. R., Angelova, P. R., Horrocks, M. H., Choi, M. L., Rodrigues, M., Baev, A. Y., Berezhnov, A. V., Yao, Z., Little, D., Banushi, B., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-04422-2 · Cited by 513
Quantitative evaluation of software packages for single-molecule localization microscopy
Sage, D., Kirshner, H., Pengo, T., Stuurman, N., Min, J., Manley, S., & Unser, M. (2015) · Nature Methods · doi:10.1038/nmeth.3442 · Cited by 396
Strategic and practical guidelines for successful structured illumination microscopy
Demmerle, J., Innocent, C., North, A. J., Ball, G., Müller, M., Miron, E., Matsuda, A., Dobbie, I. M., Markaki, Y., & Schermelleh, L. (2017) · Nature Protocols · doi:10.1038/nprot.2017.019 · Cited by 336
Switchable Fluorophores for Single-Molecule Localization Microscopy
Li, H., & Vaughan, J. C. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00767 · Cited by 319
Metadata
- Authors
- Nieuwenhuizen, R. P. J., Lidke, K. A., Bates, M., Puig, D. L., Grünwald, D., Stallinga, S., & Rieger, B.
- Venue
- Nature Methods
- Published
- 2013-04-28
- Volume
- 10
- Issue
- 6
- Pages
- 557-562
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2134396482
- Cited by
- 800
- References
- 2
- FWCI
- 24.48
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Nieuwenhuizen, R. P. J., Lidke, K. A., Bates, M., Puig, D. L., Grünwald, D., Stallinga, S., & Rieger, B. (2013). Measuring image resolution in optical nanoscopy. Nature Methods. https://doi.org/10.1038/nmeth.2448
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