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Cited by 618

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Integrated proteomic analysis of post-translational modifications by serial enrichment

Open access
Mertins, Philipp, Qiao, Jana W, Patel, Jinal, Udeshi, Namrata D, Clauser, Karl R, Mani, D R, Burgess, Michael W, Gillette, Michael A, Jaffe, Jacob D, Carr, Steven A·2013·Nature Methods

Abstract

We report a mass spectrometry–based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.

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Crossref · 10.1038/nmeth.2518

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Metadata

Authors
Mertins, P., Qiao, J. W., Patel, J., Udeshi, N. D., Clauser, K. R., Mani, D. R., Burgess, M. W., Gillette, M. A., Jaffe, J. D., & Carr, S. A.
Venue
Nature Methods
Published
2013-06-09
Volume
10
Issue
7
Pages
634-637
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
OpenAlex
W1974515407
Cited by
618
References
1
FWCI
21.70
Type
journal article
Language
en
Open access
green

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