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Near-infrared fluorescent proteins for multicolor in vivo imaging
Open accessAbstract
Near-infrared fluorescent proteins (FPs) are in high demand for in vivo imaging. We developed four spectrally distinct near-infrared FPs—iRFP670, iRFP682, iRFP702 and iRFP720—from bacterial phytochromes. iRFPs exhibit high brightness in mammalian cells and tissues and are suitable for long-term studies. iRFP670 and iRFP720 enable two-color imaging with standard approaches in living cells and mice. The four new iRFPs and the previously engineered iRFP713 allow multicolor imaging with spectral unmixing in living mice.
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A clearer vision for in vivo imaging
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Metadata
- Authors
- Shcherbakova, D. M., & Verkhusha, V. V.
- Venue
- Nature Methods
- Published
- 2013-06-16
- Volume
- 10
- Issue
- 8
- Pages
- 751-754
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- OpenAlex
- W2009283961
- Cited by
- 541
- References
- 1
- FWCI
- 26.19
- Type
- journal article
- Language
- en
- Open access
- green
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