Cited by 597Optical Imaging and Spectroscopy TechniquesMolecular Sensors and Ion DetectionSpectroscopy Techniques in Biomedical and Chemical Research

In vivo NIR-II fluorescence imaging for biology and medicine

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Wang, Feifei, Zhong, Yeteng, Bruns, Oliver, Liang, Yongye, Dai, Hongjie·2024·Nature Photonics

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Crossref · 10.1038/s41566-024-01391-5

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119 of 119 references verified0 citation errors · 0 not checkable

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  • NIR-II fluorophores have deep-tissue penetration, reduced tissue autofluorescence, and low phototoxicity.

    “Near-infrared II (NIR-II) fluorophores possess transformative potential for biomedical applications, owing to their deep-tissue penetration, reduced tissue autofluorescence, and low phototoxicity.”
  • The reduced absorption and scattering of NIR-II light suppresses background noise, minimizes tissue autofluorescence, and results in negligible phototoxicity.

    “This fundamental property suppresses background noise, minimizes tissue autofluorescence, and results in negligible phototoxicity39,40.”
  • The NIR-II window can be divided into NIR-IIa (1300-1400 nm) and NIR-IIb (1500-1700 nm), with NIR-IIb typically offering more advantageous imaging performance.

    “Notably, the NIR-II window can be further divided into the NIR-IIa (1300–1400 nm), NIR-IIb (1500–1700 nm) sub-windows, with the latter typically offering even more advantageous imaging performance39.”

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Authors
Wang, F., Zhong, Y., Bruns, O., Liang, Y., & Dai, H.
Affiliations
  • University of Hong Kong (HK)
  • Chinese Academy of Sciences (CN)
  • Southern University of Science and Technology (CN)
Corresponding author
Dai, Hongjie
Venue
Nature Photonics
Published
2024-03-04
Volume
18
Issue
6
Pages
535-547
ISSN
  • 1749-4885 (ISSN-L)
  • 1749-4893
OpenAlex
W4392376294
Cited by
564
References
119
Citations by year
2026: 227 · 2025: 267 · 2024: 70
FWCI
143.68
Type
journal article
Language
en
Open access
Locations
  • Nature Photonics (publishedVersion)
  • The HKU Scholars Hub (University of Hong Kong) (submittedVersion, cc-by-nc-nd)
  • PuSH - Publication Server of Helmholtz Zentrum München (submittedVersion)
Funders
U.S. Department of Health & Human Services | NIH | Center for Scientific Review (DP1-NS-105737)
Topics
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
  • Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
Keywords
NIR-II fluorescence imaging, fluorophore design, fluorescent probes, molecular imaging, single-cell imaging, wide-field imaging, fluorescence microscopy, small animal imaging, clinical translation, tissue autofluorescence, light scattering

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